Anti-Adiponectin antibody (ab216502)
Key features and details
- Rabbit polyclonal to Adiponectin
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Adiponectin antibody
See all Adiponectin primary antibodies -
Description
Rabbit polyclonal to Adiponectin -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Adiponectin aa 140-190 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q15848 -
Positive control
- IHC-P: Human colon carcinoma and mouse liver tissues. WB: Rat liver, mouse intestine, heart and spleen lysates. HepG2 cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab216502 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/500.
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WB |
1/100 - 1/1000. Predicted molecular weight: 26 kDa.
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Notes |
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IHC-P
1/100 - 1/500. |
WB
1/100 - 1/1000. Predicted molecular weight: 26 kDa. |
Target
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Function
Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. -
Tissue specificity
Synthesized exclusively by adipocytes and secreted into plasma. -
Involvement in disease
Defects in ADIPOQ are the cause of adiponectin deficiency (ADPND) [MIM:612556]. ADPND results in very low concentrations of plasma adiponectin.
Genetic variations in ADIPOQ are associated with non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance. -
Sequence similarities
Contains 1 C1q domain.
Contains 1 collagen-like domain. -
Domain
The C1q domain is commonly called the globular domain. -
Post-translational
modificationsHydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting.
HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes.
O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 9370 Human
- Entrez Gene: 11450 Mouse
- Entrez Gene: 246253 Rat
- Omim: 605441 Human
- SwissProt: Q15848 Human
- SwissProt: Q60994 Mouse
- Unigene: 80485 Human
- Unigene: 3969 Mouse
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Alternative names
- 30 kDa adipocyte complement related protein antibody
- 30 kDa adipocyte complement-related protein antibody
- ACDC antibody
see all
Images
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Anti-Adiponectin antibody (ab216502) at 1/300 dilution + HepG2 cell lysate
Secondary
Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/5000 dilution
Predicted band size: 26 kDa -
Anti-Adiponectin antibody (ab216502) at 1/300 dilution + Mouse spleen lysate
Secondary
Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/5000 dilution
Predicted band size: 26 kDa -
Anti-Adiponectin antibody (ab216502) at 1/300 dilution + mouse heart lysate
Secondary
Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/5000 dilution
Predicted band size: 26 kDa -
All lanes : Anti-Adiponectin antibody (ab216502) at 1/200 dilution
Lane 1 : rat liver lysate
Lane 2 : mouse intestine lysate
Secondary
All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/3000 dilution
Predicted band size: 26 kDa -
Immunohistochemical analysis of formalin-fixed and paraffin embedded mouse liver tissue labeling Adiponectin using ab216502 at 1/200, followed by secondary detection and DAB staining.
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Immunohistochemical analysis of formalin-fixed and paraffin embedded human colon carcinoma tissue labeling Adiponectin using ab216502 at 1/200, followed by secondary detection and DAB staining.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab216502 has not yet been referenced specifically in any publications.