Overview

  • Product name

  • Description

    Rabbit polyclonal to Adiponectin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Adiponectin aa 140-190 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: Q15848

  • Positive control

    • IHC-P: Human colon carcinoma and mouse liver tissues. WB: Rat liver, mouse intestine, heart and spleen lysates. HepG2 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab216502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.
WB 1/100 - 1/1000. Predicted molecular weight: 26 kDa.

Target

  • Function

    Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
  • Tissue specificity

    Synthesized exclusively by adipocytes and secreted into plasma.
  • Involvement in disease

    Defects in ADIPOQ are the cause of adiponectin deficiency (ADPND) [MIM:612556]. ADPND results in very low concentrations of plasma adiponectin.
    Genetic variations in ADIPOQ are associated with non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
  • Sequence similarities

    Contains 1 C1q domain.
    Contains 1 collagen-like domain.
  • Domain

    The C1q domain is commonly called the globular domain.
  • Post-translational
    modifications

    Hydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting.
    HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes.
    O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • 30 kDa adipocyte complement related protein antibody
    • 30 kDa adipocyte complement-related protein antibody
    • ACDC antibody
    • Acrp 30 antibody
    • ACRP30 antibody
    • ADIPO_HUMAN antibody
    • Adipocyte antibody
    • Adipocyte C1q and collagen domain containing protein antibody
    • Adipocyte complement related 30 kDa protein antibody
    • Adipocyte complement related protein of 30 kDa antibody
    • Adipocyte complement-related 30 kDa protein antibody
    • adipocyte-specific secretory protein antibody
    • Adiponectin antibody
    • Adiponectin precursor antibody
    • adiponectin, C1Q and collagen domain containing antibody
    • Adipoq antibody
    • Adipose most abundant gene transcript 1 antibody
    • Adipose most abundant gene transcript 1 protein antibody
    • Adipose specific collagen like factor antibody
    • ADIPQTL1 antibody
    • ADPN antibody
    • APM 1 antibody
    • apM-1 antibody
    • APM1 antibody
    • C1q and collagen domain-containing protein antibody
    • GBP 28 antibody
    • GBP28 antibody
    • Gelatin binding protein antibody
    • Gelatin binding protein 28 antibody
    • gelatin-binding protein 28 antibody
    • Gelatin-binding protein antibody
    • OTTHUMP00000210047 antibody
    see all

Images

  • Anti-Adiponectin antibody (ab216502) at 1/300 dilution + HepG2 cell lysate

    Secondary
    Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/5000 dilution

    Predicted band size: 26 kDa

  • Anti-Adiponectin antibody (ab216502) at 1/300 dilution + Mouse spleen lysate

    Secondary
    Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/5000 dilution

    Predicted band size: 26 kDa

  • Anti-Adiponectin antibody (ab216502) at 1/300 dilution + mouse heart lysate

    Secondary
    Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/5000 dilution

    Predicted band size: 26 kDa

  • All lanes : Anti-Adiponectin antibody (ab216502) at 1/200 dilution

    Lane 1 : rat liver lysate
    Lane 2 : mouse intestine lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/3000 dilution

    Predicted band size: 26 kDa

  • Immunohistochemical analysis of formalin-fixed and paraffin embedded mouse liver tissue labeling Adiponectin using ab216502 at 1/200, followed by secondary detection and DAB staining.

  • Immunohistochemical analysis of formalin-fixed and paraffin embedded human colon carcinoma tissue labeling Adiponectin using ab216502 at 1/200, followed by secondary detection and DAB staining.

References

ab216502 has not yet been referenced specifically in any publications.

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