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    adiponectin-antibody-ab62551.pdf

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Cardiovascular Lipids / Lipoproteins Adipose Related Acrp
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Anti-Adiponectin antibody (ab62551)

  • Datasheet
Reviews (1)Q&A (8)References (10)

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Western blot - Anti-Adiponectin antibody (ab62551)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adiponectin antibody (ab62551)

Key features and details

  • Rabbit polyclonal to Adiponectin
  • Suitable for: WB, IHC-P
  • Reacts with: Rat
  • Isotype: IgG

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Overview

  • Product name

    Anti-Adiponectin antibody
    See all Adiponectin primary antibodies
  • Description

    Rabbit polyclonal to Adiponectin
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Rat
    WB
    Rat
    See all applications and species data
  • Immunogen

    A 15 amino acid peptide from near the amino terminus of human Adiponectin.

  • Positive control

    • Rat brain cell lysate. Rat brain tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Adipose Related
    • Acrp
    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Neuroscience
    • Neurology process
    • Metabolism
    • Stem Cells
    • Mesenchymal Stem Cells
    • Adipogenesis
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Diabetes
    • Metabolism
    • Types of disease
    • Obesity
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Types of disease
    • Heart disease
    • Metabolism
    • Types of disease
    • Metabolic disorders

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Adiponectin protein (ab51294)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab62551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Rat
WB
Rat
Application Abreviews Notes
WB (1)
Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 26 kDa).
IHC-P
Use a concentration of 1 µg/ml.
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 26 kDa).
IHC-P
Use a concentration of 1 µg/ml.

Target

  • Function

    Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
  • Tissue specificity

    Synthesized exclusively by adipocytes and secreted into plasma.
  • Involvement in disease

    Defects in ADIPOQ are the cause of adiponectin deficiency (ADPND) [MIM:612556]. ADPND results in very low concentrations of plasma adiponectin.
    Genetic variations in ADIPOQ are associated with non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
  • Sequence similarities

    Contains 1 C1q domain.
    Contains 1 collagen-like domain.
  • Domain

    The C1q domain is commonly called the globular domain.
  • Post-translational
    modifications

    Hydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting.
    HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes.
    O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession Q15848 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 246253 Rat
    • Alternative names

      • 30 kDa adipocyte complement related protein antibody
      • 30 kDa adipocyte complement-related protein antibody
      • ACDC antibody
      • Acrp 30 antibody
      • ACRP30 antibody
      • ADIPO_HUMAN antibody
      • Adipocyte antibody
      • Adipocyte C1q and collagen domain containing protein antibody
      • Adipocyte complement related 30 kDa protein antibody
      • Adipocyte complement related protein of 30 kDa antibody
      • Adipocyte complement-related 30 kDa protein antibody
      • adipocyte-specific secretory protein antibody
      • Adiponectin antibody
      • Adiponectin precursor antibody
      • adiponectin, C1Q and collagen domain containing antibody
      • Adipoq antibody
      • Adipose most abundant gene transcript 1 antibody
      • Adipose most abundant gene transcript 1 protein antibody
      • Adipose specific collagen like factor antibody
      • ADIPQTL1 antibody
      • ADPN antibody
      • APM 1 antibody
      • apM-1 antibody
      • APM1 antibody
      • C1q and collagen domain-containing protein antibody
      • GBP 28 antibody
      • GBP28 antibody
      • Gelatin binding protein antibody
      • Gelatin binding protein 28 antibody
      • gelatin-binding protein 28 antibody
      • Gelatin-binding protein antibody
      • OTTHUMP00000210047 antibody
      see all

    Images

    • Western blot - Anti-Adiponectin antibody (ab62551)
      Western blot - Anti-Adiponectin antibody (ab62551)
      Lane 1 : Anti-Adiponectin antibody (ab62551) at 1 µg/ml
      Lane 2 : Anti-Adiponectin antibody (ab62551) at 2 µg/ml
      Lane 3 : Anti-Adiponectin antibody (ab62551) at 4 µg/ml

      All lanes : Rat brain cell lysate

      Lysates/proteins at 15 µg per lane.

      Predicted band size: 26 kDa
      Observed band size: 32 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adiponectin antibody (ab62551)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adiponectin antibody (ab62551)
      ab62551, at 1µg/ml, staining Adiponectin in rat brain tissue by immunohistochemistry using paraffin-embedded tissue.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (10)

    Publishing research using ab62551? Please let us know so that we can cite the reference in this datasheet.

    ab62551 has been referenced in 10 publications.

    • Hsu MH  et al. Effects of Maternal Resveratrol on Maternal High-Fat Diet/Obesity with or without Postnatal High-Fat Diet. Int J Mol Sci 21:N/A (2020). PubMed: 32408716
    • Zhan Y  et al. Aspirin-induced attenuation of adipogenic differentiation of bone marrow mesenchymal stem cells is accompanied by the disturbed epigenetic modification. Int J Biochem Cell Biol 98:29-42 (2018). WB . PubMed: 29471042
    • Zhang Y  et al. miR-450a-5p within rat adipose tissue exosome-like vesicles promotes adipogenic differentiation by targeting WISP2. J Cell Sci 130:1158-1168 (2017). PubMed: 28167681
    • Tang Q  et al. Botulinum toxin A improves adipose tissue engraftment by promoting cell proliferation, adipogenesis and angiogenesis. Int J Mol Med 40:713-720 (2017). PubMed: 28731141
    • Kim TK  et al. Melatonin modulates adiponectin expression on murine colitis with sleep deprivation. World J Gastroenterol 22:7559-68 (2016). IHC-P ; Mouse . PubMed: 27672276
    • Favero G  et al. Melatonin reduces obesity and restores adipokine patterns and metabolism in obese (ob/ob) mice. Nutr Res 35:891-900 (2015). IF ; Mouse . PubMed: 26250620
    • Kabara E  et al. Adiponectin links adipose tissue function and monocyte inflammatory responses during bovine metabolic stress. Comp Immunol Microbiol Infect Dis 37:49-58 (2014). PubMed: 24296305
    • Anderson NJ  et al. Role of insulin signaling impairment, adiponectin and dyslipidemia in peripheral and central neuropathy in mice. Dis Model Mech 7:625-33 (2014). WB ; Mouse . PubMed: 24764191
    • Batista ML  et al. Heterogeneous time-dependent response of adipose tissue during the development of cancer cachexia. J Endocrinol 215:363-73 (2012). WB ; Rat . PubMed: 23033362
    • Ortega HH  et al. Developmental programming: effect of prenatal steroid excess on intraovarian components of insulin signaling pathway and related proteins in sheep. Biol Reprod 82:1065-75 (2010). PubMed: 20147730

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-9 of 9 Abreviews or Q&A

    Question

    Our lab bought ab62551 one year ago. At first, it was no problem to use this primary antibody to detect adiponectin. But right now, we cannot detect adiponectin from our samples under the same conditions. In the membrane, it only can show the bands at 40 kDa. I do not know what is wrong with this primary antibody. By the way, we had stored the ab62551 at 4 degree for half an year and now we store it at -20 degree.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 15 2013

    Answer

    I am very sorry to hear that this product has been performing in the matter you have stated. It is likely that the antibody has degraded with time and temperature. This product should be stored a +4C. Items which require this storage temperature generally have a lifespan of ~1 year. It is likely that moving the product to -20C accelerated this degradation further as we know the product does not handle these temperatures well. Abcam does offer our 6 month Abpromise guarantee. We will support, replace or refund any item within this Abpromise period provide it is used as stated on the datasheet.

    Read More

    Abcam Scientific Support

    Answered on Feb 16 2013

    Question

    Detecting multiple bands in blots of rat kidney cortex.

    Read More

    Abcam community

    Verified customer

    Asked on May 01 2012

    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number . To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research. Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number [XXXXX]. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    Answered on May 01 2012

    Question

    Thank you very much for your mail. As we have discussed on the phone, I shall try a different blocking solution and see if it does any good. Meanwhile, I have attached a scanned copy of the Adiponectin blot that I had problems with. Also, I have obtained proper results with the GAPDH control when run under the same conditions. Please go through the attachment and let me know if there is anything more that you can help with.

    Read More

    Abcam community

    Verified customer

    Asked on Apr 24 2012

    Answer

    Thank you for sending the blot image. You might expect to detect a smeared band, for example the bands shown in the blot for antibody ab85827, given that there may be varying amounts of glycosylation within a single sample, but there are too many bands in your ab62551 blot to allow identification of any of them as adiponectin.

    Click here (or use the following: https://www.abcam.com/Adiponectin-antibody-ab85827.html).

    The blocking modification may help but if not, please reply to this e-mail.

    Read More

    Abcam Scientific Support

    Answered on Apr 24 2012

    Question

    Detecting multiple bands in blots of rat kidney cortex.

    Read More

    Abcam community

    Verified customer

    Asked on Apr 24 2012

    Answer

    Thank you for contacting us and bringing to our attention the difficulty you are having identifying adiponectin in your blots. If you can send an image of you blot or blots as an attachment in your reply, I may be able to help you more effectively.

    As I mentioned, a change in blocking conditions may develop the specific signal for adiponectin more than the bands that represent proteins the antibody is cross-reactiing with. Washing more thoroughly may also help, but I assume you are already washing at least three times for several minutes after incubation of the blot with ab62551.

    If you are interested, we have purified recombinant rat adiponectin which could serve as a useful standard. The catalogue number is ab52151:

    Click here (or use the following: https://www.abcam.com/Recombinant-Rat-Adiponectin-protein-ab52151.html).

    However, it will not be glycosylated, so it is likely to run lower on the gel than your native adiponectin, at 26 kDa.

    Read More

    Abcam Scientific Support

    Answered on Apr 24 2012

    Western blot abreview for Anti-Adiponectin antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Cow Serum (Plasma diluted 1:25)
    Loading amount
    15 µg
    Specification
    Plasma diluted 1:25
    Treatment
    DTT and BME for 1 hour and boiled for 10 min
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 minute(s) · Concentration: 0.1% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 17 2012

    Question

    I've submitted my abreview of the product. Is that allI have to do for the discount to be applied?
    Sincerely,

    Read More

    Abcam community

    Verified customer

    Asked on Apr 09 2012

    Answer

    Thank you for your reply.
    My colleague is out of the office but I will be happy to help you with this enquiry.
    Yes, submitting the Abreview is all that you need to do in order to active the discount. I have added the discount code to our internal notes section of the Abreview for ab62551, so just include this discount code with your next order to Abcam, and you will receive a 100% discount off of a primary antibody.
    I hope this information will be useful, but please let me know if you have any further questions or if there is anything else that we can do for you.

    Read More

    Abcam Scientific Support

    Answered on Apr 09 2012

    Question

    Thank you for the discount codes, but we wanted to try protein ab6361 and antibody ab62551. Will these codes for for those antibodies and proteins?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 29 2012

    Answer

    Thank you for your reply. The catalog number ab6361 refers to an unpublished antibody, so I am assuming you meant ab63261 the tagged Adiponectin protein? The following discount codes can be used for testing the products you are interested in. Please let me know if you need any additional assistance.
    For ab63261: ***

    For ab62551: ***
    Expiration Date: July 29th, 2012

    Read More

    Abcam Scientific Support

    Answered on Mar 29 2012

    Question

    Dear Sirs


    We kindly ask you to send us the expiry date for the following items:

    1 pcs Each


    ab62551 Anti-Adiponectin antibody 100 micrograms


    100µg


    ab19481 Anti-PPAR gamma antibody 200 micrograms


    200µg

    Thank you in advance.


    Best regards

    Read More

    Abcam community

    Verified customer

    Asked on Jan 30 2012

    Answer

    Thank you for your enquiry.

    We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage instructions on the datasheet for the antibody you purchased. These conditions will vary among our antibodies, therefore, it is important to verify the storage conditions for each of our products when you receive them. We guarantee all of our products to work for at leastsix monthfrom the date of purchase when stored correctly.

    For more information on antibody storage and stability, please visit our Antibody Storage Guide on our Protocols and Troubleshooting Tips page (https://www.abcam.com/index.html?pageconfig=popular_protocols).

    I hope this information helps. Please do not hesitate to contact us if you need anything further.

    Read More

    Abcam Scientific Support

    Answered on Jan 30 2012

    Question

    Dear technical team,   Our customer would like to purchase ab62551 and ab16227. She is going to test IHC-P with human sample and needs positive control for those two antibodies. How about ab4616 for ab62551 and ab4360 for ab16227?   Please check this for our customer.   Thank you.   Best regards,

    Read More

    Abcam community

    Verified customer

    Asked on Jul 29 2011

    Answer

    Thank you for your enquiry. I have just quickly checked the datahseets and the specificity of these products for you and can confirm that these slides can be used as positive control. - ab4616 for ab62551 and - ab4360 for ab16227 I would suggest following the staining procedure (provided in the legend og the IHC image) and perhaps applying signal enhancing step (Dako Envision Flex amplification or ABC system). I hope this helps and if I can assist further, please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    Answered on Jul 29 2011

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    For licensing inquiries, please contact partnerships@abcam.com

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