• Product name
  • Description
    Rabbit polyclonal to Adiponectin
  • Host species
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 15 amino acid peptide from near the amino terminus of human Adiponectin.

  • Positive control
    • Rat brain cell lysate. Rat brain tissue.


Our Abpromise guarantee covers the use of ab62551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 26 kDa).
IHC-P Use a concentration of 1 µg/ml.


  • Function
    Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
  • Tissue specificity
    Synthesized exclusively by adipocytes and secreted into plasma.
  • Involvement in disease
    Defects in ADIPOQ are the cause of adiponectin deficiency (ADPND) [MIM:612556]. ADPND results in very low concentrations of plasma adiponectin.
    Genetic variations in ADIPOQ are associated with non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
  • Sequence similarities
    Contains 1 C1q domain.
    Contains 1 collagen-like domain.
  • Domain
    The C1q domain is commonly called the globular domain.
  • Post-translational
    Hydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting.
    HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes.
    O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • 30 kDa adipocyte complement related protein antibody
    • 30 kDa adipocyte complement-related protein antibody
    • ACDC antibody
    • ACRP30 antibody
    • ADIPO_HUMAN antibody
    • Adipocyte antibody
    • Adipocyte C1q and collagen domain containing protein antibody
    • Adipocyte complement related 30 kDa protein antibody
    • Adipocyte complement related protein of 30 kDa antibody
    • Adipocyte complement-related 30 kDa protein antibody
    • adipocyte-specific secretory protein antibody
    • Adiponectin antibody
    • Adiponectin precursor antibody
    • adiponectin, C1Q and collagen domain containing antibody
    • Adipoq antibody
    • Adipose most abundant gene transcript 1 antibody
    • Adipose most abundant gene transcript 1 protein antibody
    • Adipose specific collagen like factor antibody
    • ADIPQTL1 antibody
    • ADPN antibody
    • APM 1 antibody
    • apM-1 antibody
    • APM1 antibody
    • C1q and collagen domain-containing protein antibody
    • GBP28 antibody
    • Gelatin binding protein antibody
    • Gelatin binding protein 28 antibody
    • Gelatin-binding protein antibody
    see all


  • Lane 1 : Anti-Adiponectin antibody (ab62551) at 1 µg/ml
    Lane 2 : Anti-Adiponectin antibody (ab62551) at 2 µg/ml
    Lane 3 : Anti-Adiponectin antibody (ab62551) at 4 µg/ml

    All lanes : Rat brain cell lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 26 kDa
    Observed band size: 32 kDa (why is the actual band size different from the predicted?)

  • ab62551, at 1µg/ml, staining Adiponectin in rat brain tissue by immunohistochemistry using paraffin-embedded tissue.


This product has been referenced in:
  • Zhan Y  et al. Aspirin-induced attenuation of adipogenic differentiation of bone marrow mesenchymal stem cells is accompanied by the disturbed epigenetic modification. Int J Biochem Cell Biol 98:29-42 (2018). WB . Read more (PubMed: 29471042) »
  • Tang Q  et al. Botulinum toxin A improves adipose tissue engraftment by promoting cell proliferation, adipogenesis and angiogenesis. Int J Mol Med 40:713-720 (2017). Read more (PubMed: 28731141) »

See all 8 Publications for this product

Customer reviews and Q&As

I am very sorry to hear that this product has been performing in the matter you have stated. It is likely that the antibody has degraded with time and temperature. This product should be stored a +4C. Items which require this storage temperature genera...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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Thank you for sending the blot image. You might expect to detect a smeared band, for example the bands shown in the blot for antibody ab85827, given that there may be varying amounts of glycosylation within a single sample, but there are too many bands...

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Thank you for contacting us and bringing to our attention the difficulty you are having identifying adiponectin in your blots. If you can send an image of you blot or blots as an attachment in your reply, I may be able to help you more effectively. Read More

Western blot
Cow Serum (Plasma diluted 1:25)
Loading amount
15 µg
Plasma diluted 1:25
DTT and BME for 1 hour and boiled for 10 min
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 minute(s) · Concentration: 0.1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 17 2012

Thank you for your reply.
My colleague is out of the office but I will be happy to help you with this enquiry.
Yes, submitting the Abreview is all that you need to do in order to active the discount. I have added the discount code to our inte...

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Thank you for your reply. The catalog number ab6361 refers to an unpublished antibody, so I am assuming you meant ab63261 the tagged Adiponectin protein? The following discount codes can be used for testing the products you are interested in. Please le...

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Thank you for your enquiry.

We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storag...

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Thank you for your enquiry. I have just quickly checked the datahseets and the specificity of these products for you and can confirm that these slides can be used as positive control. - ab4616 for ab62551 and - ab4360 for ab16227 I would sug...

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