Anti-Adiponectin antibody (ab62551)

I am very sorry to hear that this product has been performing in the matter you have stated. It is likely that the antibody has degraded with time and temperature. This product should be stored a +4C. Items which require this storage temperature generally have a lifespan of ~1 year. It is likely that moving the product to -20C accelerated this degradation further as we know the product does not handle these temperatures well. Abcam does offer our 6 month Abpromise guarantee. We will support, replace or refund any item within this Abpromise period provide it is used as stated on the datasheet.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number . To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research. Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number [XXXXX]. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

Thank you for sending the blot image. You might expect to detect a smeared band, for example the bands shown in the blot for antibody ab85827, given that there may be varying amounts of glycosylation within a single sample, but there are too many bands in your ab62551 blot to allow identification of any of them as adiponectin.

Click here (or use the following: https://www.abcam.com/Adiponectin-antibody-ab85827.html).

The blocking modification may help but if not, please reply to this e-mail.

Thank you for contacting us and bringing to our attention the difficulty you are having identifying adiponectin in your blots. If you can send an image of you blot or blots as an attachment in your reply, I may be able to help you more effectively.

As I mentioned, a change in blocking conditions may develop the specific signal for adiponectin more than the bands that represent proteins the antibody is cross-reactiing with. Washing more thoroughly may also help, but I assume you are already washing at least three times for several minutes after incubation of the blot with ab62551.

If you are interested, we have purified recombinant rat adiponectin which could serve as a useful standard. The catalogue number is ab52151:

Click here (or use the following: https://www.abcam.com/Recombinant-Rat-Adiponectin-protein-ab52151.html).

However, it will not be glycosylated, so it is likely to run lower on the gel than your native adiponectin, at 26 kDa.

Thank you for your reply.
My colleague is out of the office but I will be happy to help you with this enquiry.
Yes, submitting the Abreview is all that you need to do in order to active the discount. I have added the discount code to our internal notes section of the Abreview for ab62551, so just include this discount code with your next order to Abcam, and you will receive a 100% discount off of a primary antibody.
I hope this information will be useful, but please let me know if you have any further questions or if there is anything else that we can do for you.

Thank you for your reply. The catalog number ab6361 refers to an unpublished antibody, so I am assuming you meant ab63261 the tagged Adiponectin protein? The following discount codes can be used for testing the products you are interested in. Please let me know if you need any additional assistance.
For ab63261: ***

For ab62551: ***
Expiration Date: July 29th, 2012

Thank you for your enquiry.

We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage instructions on the datasheet for the antibody you purchased. These conditions will vary among our antibodies, therefore, it is important to verify the storage conditions for each of our products when you receive them. We guarantee all of our products to work for at leastsix monthfrom the date of purchase when stored correctly.

For more information on antibody storage and stability, please visit our Antibody Storage Guide on our Protocols and Troubleshooting Tips page (https://www.abcam.com/index.html?pageconfig=popular_protocols).

I hope this information helps. Please do not hesitate to contact us if you need anything further.

Thank you for your enquiry. I have just quickly checked the datahseets and the specificity of these products for you and can confirm that these slides can be used as positive control. - ab4616 for ab62551 and - ab4360 for ab16227 I would suggest following the staining procedure (provided in the legend og the IHC image) and perhaps applying signal enhancing step (Dako Envision Flex amplification or ABC system). I hope this helps and if I can assist further, please do not hesitate to contact me.