Anti-Adiponectin antibody (ab62551)
Key features and details
- Rabbit polyclonal to Adiponectin
- Suitable for: WB, IHC-P
- Reacts with: Rat
- Isotype: IgG
Overview
-
Product name
Anti-Adiponectin antibody
See all Adiponectin primary antibodies -
Description
Rabbit polyclonal to Adiponectin -
Host species
Rabbit -
Tested Applications & Species
Application Species IHC-P RatWB Rat -
Immunogen
A 15 amino acid peptide from near the amino terminus of human Adiponectin.
-
Positive control
- Rat brain cell lysate. Rat brain tissue.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab62551 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
IHC-P |
Rat
|
WB |
Rat
|
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 26 kDa).
|
IHC-P |
Use a concentration of 1 µg/ml.
|
Notes |
---|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 26 kDa). |
IHC-P
Use a concentration of 1 µg/ml. |
Target
-
Function
Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. -
Tissue specificity
Synthesized exclusively by adipocytes and secreted into plasma. -
Involvement in disease
Defects in ADIPOQ are the cause of adiponectin deficiency (ADPND) [MIM:612556]. ADPND results in very low concentrations of plasma adiponectin.
Genetic variations in ADIPOQ are associated with non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance. -
Sequence similarities
Contains 1 C1q domain.
Contains 1 collagen-like domain. -
Domain
The C1q domain is commonly called the globular domain. -
Post-translational
modificationsHydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting.
HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes.
O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
-
Alternative names
- 30 kDa adipocyte complement related protein antibody
- 30 kDa adipocyte complement-related protein antibody
- ACDC antibody
see all
Images
-
Lane 1 : Anti-Adiponectin antibody (ab62551) at 1 µg/ml
Lane 2 : Anti-Adiponectin antibody (ab62551) at 2 µg/ml
Lane 3 : Anti-Adiponectin antibody (ab62551) at 4 µg/ml
All lanes : Rat brain cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 26 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adiponectin antibody (ab62551)ab62551, at 1µg/ml, staining Adiponectin in rat brain tissue by immunohistochemistry using paraffin-embedded tissue.
Protocols
Datasheets and documents
References (10)
ab62551 has been referenced in 10 publications.
- Hsu MH et al. Effects of Maternal Resveratrol on Maternal High-Fat Diet/Obesity with or without Postnatal High-Fat Diet. Int J Mol Sci 21:N/A (2020). PubMed: 32408716
- Zhan Y et al. Aspirin-induced attenuation of adipogenic differentiation of bone marrow mesenchymal stem cells is accompanied by the disturbed epigenetic modification. Int J Biochem Cell Biol 98:29-42 (2018). WB . PubMed: 29471042
- Zhang Y et al. miR-450a-5p within rat adipose tissue exosome-like vesicles promotes adipogenic differentiation by targeting WISP2. J Cell Sci 130:1158-1168 (2017). PubMed: 28167681
- Tang Q et al. Botulinum toxin A improves adipose tissue engraftment by promoting cell proliferation, adipogenesis and angiogenesis. Int J Mol Med 40:713-720 (2017). PubMed: 28731141
- Kim TK et al. Melatonin modulates adiponectin expression on murine colitis with sleep deprivation. World J Gastroenterol 22:7559-68 (2016). IHC-P ; Mouse . PubMed: 27672276
- Favero G et al. Melatonin reduces obesity and restores adipokine patterns and metabolism in obese (ob/ob) mice. Nutr Res 35:891-900 (2015). IF ; Mouse . PubMed: 26250620
- Kabara E et al. Adiponectin links adipose tissue function and monocyte inflammatory responses during bovine metabolic stress. Comp Immunol Microbiol Infect Dis 37:49-58 (2014). PubMed: 24296305
- Anderson NJ et al. Role of insulin signaling impairment, adiponectin and dyslipidemia in peripheral and central neuropathy in mice. Dis Model Mech 7:625-33 (2014). WB ; Mouse . PubMed: 24764191
- Batista ML et al. Heterogeneous time-dependent response of adipose tissue during the development of cancer cachexia. J Endocrinol 215:363-73 (2012). WB ; Rat . PubMed: 23033362
- Ortega HH et al. Developmental programming: effect of prenatal steroid excess on intraovarian components of insulin signaling pathway and related proteins in sheep. Biol Reprod 82:1065-75 (2010). PubMed: 20147730