- Datasheet
- Specific References (8)
- Protocols
Overview
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Product nameAnti-Adiponectin antibody
See all Adiponectin primary antibodies -
DescriptionRabbit polyclonal to Adiponectin
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-Pmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
A 15 amino acid peptide from near the amino terminus of human Adiponectin.
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Positive control
- Rat brain cell lysate. Rat brain tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C.
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Storage bufferPreservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab62551 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 26 kDa). | |
| IHC-P | Use a concentration of 1 µg/ml. |
Target
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FunctionImportant adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
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Tissue specificitySynthesized exclusively by adipocytes and secreted into plasma.
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Involvement in diseaseDefects in ADIPOQ are the cause of adiponectin deficiency (ADPND) [MIM:612556]. ADPND results in very low concentrations of plasma adiponectin.
Genetic variations in ADIPOQ are associated with non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance. -
Sequence similaritiesContains 1 C1q domain.
Contains 1 collagen-like domain. -
DomainThe C1q domain is commonly called the globular domain.
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Post-translational
modificationsHydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting.
HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes.
O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation. -
Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 9370 Human
- Entrez Gene: 11450 Mouse
- Entrez Gene: 246253 Rat
- Omim: 605441 Human
- SwissProt: Q15848 Human
- SwissProt: Q60994 Mouse
- Unigene: 80485 Human
- Unigene: 3969 Mouse
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Alternative names
- 30 kDa adipocyte complement related protein antibody
- 30 kDa adipocyte complement-related protein antibody
- ACDC antibody
see all
Images
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Western blot - Anti-Adiponectin antibody (ab62551)Lane 1 : Anti-Adiponectin antibody (ab62551) at 1 µg/ml
Lane 2 : Anti-Adiponectin antibody (ab62551) at 2 µg/ml
Lane 3 : Anti-Adiponectin antibody (ab62551) at 4 µg/ml
All lanes : Rat brain cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 26 kDa
Observed band size: 32 kDa (why is the actual band size different from the predicted?) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adiponectin antibody (ab62551)ab62551, at 1µg/ml, staining Adiponectin in rat brain tissue by immunohistochemistry using paraffin-embedded tissue.
References
This product has been referenced in:
- Zhan Y et al. Aspirin-induced attenuation of adipogenic differentiation of bone marrow mesenchymal stem cells is accompanied by the disturbed epigenetic modification. Int J Biochem Cell Biol 98:29-42 (2018). WB . Read more (PubMed: 29471042) »
- Tang Q et al. Botulinum toxin A improves adipose tissue engraftment by promoting cell proliferation, adipogenesis and angiogenesis. Int J Mol Med 40:713-720 (2017). Read more (PubMed: 28731141) »
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