Overview

  • Product name
    Anti-Adiponectin antibody (Biotin)
    See all Adiponectin primary antibodies
  • Description
    Rabbit polyclonal to Adiponectin (Biotin)
  • Host species
    Rabbit
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant full length protein (Human).

Properties

Applications

Our Abpromise guarantee covers the use of ab17895 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes

    ELISA: Use at a concentration of 0.15 - 0.3 µg/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant Acrp30.
    WB: Use at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human Acrp30 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Predicted molecular weight: 27 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
    • Tissue specificity
      Synthesized exclusively by adipocytes and secreted into plasma.
    • Involvement in disease
      Defects in ADIPOQ are the cause of adiponectin deficiency (ADPND) [MIM:612556]. ADPND results in very low concentrations of plasma adiponectin.
      Genetic variations in ADIPOQ are associated with non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
    • Sequence similarities
      Contains 1 C1q domain.
      Contains 1 collagen-like domain.
    • Domain
      The C1q domain is commonly called the globular domain.
    • Post-translational
      modifications
      Hydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting.
      HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes.
      O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • 30 kDa adipocyte complement related protein antibody
      • 30 kDa adipocyte complement-related protein antibody
      • ACDC antibody
      • ACRP30 antibody
      • ADIPO_HUMAN antibody
      • Adipocyte antibody
      • Adipocyte C1q and collagen domain containing protein antibody
      • Adipocyte complement related 30 kDa protein antibody
      • Adipocyte complement related protein of 30 kDa antibody
      • Adipocyte complement-related 30 kDa protein antibody
      • adipocyte-specific secretory protein antibody
      • Adiponectin antibody
      • Adiponectin precursor antibody
      • adiponectin, C1Q and collagen domain containing antibody
      • Adipoq antibody
      • Adipose most abundant gene transcript 1 antibody
      • Adipose most abundant gene transcript 1 protein antibody
      • Adipose specific collagen like factor antibody
      • ADIPQTL1 antibody
      • ADPN antibody
      • APM 1 antibody
      • apM-1 antibody
      • APM1 antibody
      • C1q and collagen domain-containing protein antibody
      • GBP28 antibody
      • Gelatin binding protein antibody
      • Gelatin binding protein 28 antibody
      • Gelatin-binding protein antibody
      see all

    References

    ab17895 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    Thank you for contacting us. I am sorry to confirm that because we carry over 70,000 products, it is not feasible for us to keep small sample sizes of our products to send to customers. We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase. If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information. Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates. To find out more about our Abreview system, please see the following link: https://www.abcam.com/abreviews I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

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    Answer

    Thank you for contacting us. I am happy to confirm that we do have this same antibody without Biotin. It is ab26048, Rabbit polyclonal to Adiponectin. https://www.abcam.com/index.html?datasheet=26048 I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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