Overview

  • Product name

    Anti-Adiponectin antibody - C-terminal
    See all Adiponectin primary antibodies
  • Description

    Rabbit polyclonal to Adiponectin - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ELISA, WB, IHC-P, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide corresponding to Human Adiponectin aa 224-244 (C terminal).
    Sequence:

    LYADNDNDSTFTGFLLYHDTN


    Database link: Q15848

  • Positive control

    • Rat heart tissue lysate. Recombinant Human Adiponectin protein. Rat kidney, mouse kidney, rat liver and Human placenta tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab181699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.1 - 0.5 µg/ml.
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 26 kDa.

The detection limit for Adiponectin is approximately 1ng/lane under reducing conditions.

IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use a concentration of 0.5 - 1 µg/ml.

Target

  • Function

    Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
  • Tissue specificity

    Synthesized exclusively by adipocytes and secreted into plasma.
  • Involvement in disease

    Defects in ADIPOQ are the cause of adiponectin deficiency (ADPND) [MIM:612556]. ADPND results in very low concentrations of plasma adiponectin.
    Genetic variations in ADIPOQ are associated with non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
  • Sequence similarities

    Contains 1 C1q domain.
    Contains 1 collagen-like domain.
  • Domain

    The C1q domain is commonly called the globular domain.
  • Post-translational
    modifications

    Hydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting.
    HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes.
    O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • 30 kDa adipocyte complement related protein antibody
    • 30 kDa adipocyte complement-related protein antibody
    • ACDC antibody
    • Acrp 30 antibody
    • ACRP30 antibody
    • ADIPO_HUMAN antibody
    • Adipocyte antibody
    • Adipocyte C1q and collagen domain containing protein antibody
    • Adipocyte complement related 30 kDa protein antibody
    • Adipocyte complement related protein of 30 kDa antibody
    • Adipocyte complement-related 30 kDa protein antibody
    • adipocyte-specific secretory protein antibody
    • Adiponectin antibody
    • Adiponectin precursor antibody
    • adiponectin, C1Q and collagen domain containing antibody
    • Adipoq antibody
    • Adipose most abundant gene transcript 1 antibody
    • Adipose most abundant gene transcript 1 protein antibody
    • Adipose specific collagen like factor antibody
    • ADIPQTL1 antibody
    • ADPN antibody
    • APM 1 antibody
    • apM-1 antibody
    • APM1 antibody
    • C1q and collagen domain-containing protein antibody
    • GBP 28 antibody
    • GBP28 antibody
    • Gelatin binding protein antibody
    • Gelatin binding protein 28 antibody
    • gelatin-binding protein 28 antibody
    • Gelatin-binding protein antibody
    • OTTHUMP00000210047 antibody
    see all

Images

  • Anti-Adiponectin antibody - C-terminal (ab181699) at 0.5 µg/ml + rat heart tissue lysate

    Predicted band size: 26 kDa

  • All lanes : Anti-Adiponectin antibody - C-terminal (ab181699) at 0.5 µg/ml

    Lane 1 : Recombinant Human Adiponectin protein at 0.01 µg
    Lane 2 : Recombinant Human Adiponectin protein at 0.05 µg
    Lane 3 : Recombinant Human Adiponectin protein at 0.0025 µg

    Predicted band size: 26 kDa

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue, labeling Adiponectin using ab181699 at 1 μg/ml.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue, labeling Adiponectin using ab181699 at 1 μg/ml.

  • Immunohistochemical analysis of frozen rat liver tissue, labeling Adiponectin using ab181699 at 1 μg/ml.

  • Immunohistochemical analysis of paraffin-embedded Human placenta tissue, labeling Adiponectin using ab181699 at 1 μg/ml.

References

This product has been referenced in:

  • Shen B  et al. Integrin alpha11 is an Osteolectin receptor and is required for the maintenance of adult skeletal bone mass. Elife 8:N/A (2019). Read more (PubMed: 30632962) »
See 1 Publication for this product

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