Key features and details
- Rabbit polyclonal to Adiponectin - C-terminal
- Suitable for: WB, IHC-P, IHC-Fr
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Adiponectin antibody - C-terminal
See all Adiponectin primary antibodies
DescriptionRabbit polyclonal to Adiponectin - C-terminal
Tested applicationsSuitable for: WB, IHC-P, IHC-Frmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab181699 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 26 kDa.
The detection limit for Adiponectin is approximately 1ng/lane under reducing conditions.
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IHC-Fr||Use a concentration of 0.5 - 1 µg/ml.|
FunctionImportant adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
Tissue specificitySynthesized exclusively by adipocytes and secreted into plasma.
Involvement in diseaseDefects in ADIPOQ are the cause of adiponectin deficiency (ADPND) [MIM:612556]. ADPND results in very low concentrations of plasma adiponectin.
Genetic variations in ADIPOQ are associated with non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
Sequence similaritiesContains 1 C1q domain.
Contains 1 collagen-like domain.
DomainThe C1q domain is commonly called the globular domain.
modificationsHydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting.
HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes.
O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation.
- Information by UniProt
- 30 kDa adipocyte complement related protein antibody
- 30 kDa adipocyte complement-related protein antibody
- ACDC antibody
Anti-Adiponectin antibody - C-terminal (ab181699) at 0.5 µg/ml + rat heart tissue lysate
Predicted band size: 26 kDa
All lanes : Anti-Adiponectin antibody - C-terminal (ab181699) at 0.5 µg/ml
Lane 1 : Recombinant Human Adiponectin protein at 0.01 µg
Lane 2 : Recombinant Human Adiponectin protein at 0.05 µg
Lane 3 : Recombinant Human Adiponectin protein at 0.0025 µg
Predicted band size: 26 kDa
Immunohistochemical analysis of paraffin-embedded rat kidney tissue, labeling Adiponectin using ab181699 at 1 μg/ml.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue, labeling Adiponectin using ab181699 at 1 μg/ml.
Immunohistochemical analysis of frozen rat liver tissue, labeling Adiponectin using ab181699 at 1 μg/ml.
Immunohistochemical analysis of paraffin-embedded Human placenta tissue, labeling Adiponectin using ab181699 at 1 μg/ml.
ab181699 has been referenced in 2 publications.
- Shen B et al. Integrin alpha11 is an Osteolectin receptor and is required for the maintenance of adult skeletal bone mass. Elife 8:N/A (2019). PubMed: 30632962
- Yang T et al. Wogonin enhances intracellular adiponectin levels and suppresses adiponectin secretion in 3T3-L1 adipocytes. Endocr J 64:15-26 (2017). PubMed: 27667474