Recombinant Anti-Adiponectin antibody [EPR17019] (ab181281)
![Western blot - Anti-Adiponectin antibody [EPR17019] (ab181281)](https://www.abcam.com/ps/products/181/ab181281/Images/ab181281-288747-anti-adiponectin-antibody-epr17019-western-blot.jpg "Western blot - Anti-Adiponectin antibody [EPR17019] (ab181281)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17019] to Adiponectin
- Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-Adiponectin antibody [EPR17019]
See all Adiponectin primary antibodies -
Description
Rabbit monoclonal [EPR17019] to Adiponectin -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse plasma, serum, placenta, white adipose, kidney and liver lysates. IHC-P: Mouse and rat adipose tissues. ICC/IF: 3T3-L1 cells. Flow Cyt (intra): 3T3-L1 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17019 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181281 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/50.
|
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| IHC-P |
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| WB |
1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
We don’t recommend WB for rat species because we observed an extra band around 24 kDa in addition to adiponectin, in rat plasma and rat serum lysates. |
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| ICC/IF |
1/1000.
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| Notes |
|---|
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Flow Cyt (Intra)
1/50. |
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IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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WB
1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). We don’t recommend WB for rat species because we observed an extra band around 24 kDa in addition to adiponectin, in rat plasma and rat serum lysates. |
|
ICC/IF
1/1000. |
Target
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Function
Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. -
Tissue specificity
Synthesized exclusively by adipocytes and secreted into plasma. -
Involvement in disease
Defects in ADIPOQ are the cause of adiponectin deficiency (ADPND) [MIM:612556]. ADPND results in very low concentrations of plasma adiponectin.
Genetic variations in ADIPOQ are associated with non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance. -
Sequence similarities
Contains 1 C1q domain.
Contains 1 collagen-like domain. -
Domain
The C1q domain is commonly called the globular domain. -
Post-translational
modificationsHydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting.
HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes.
O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 11450 Mouse
- Entrez Gene: 246253 Rat
- SwissProt: Q60994 Mouse
- Unigene: 3969 Mouse
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Alternative names
- 30 kDa adipocyte complement related protein antibody
- 30 kDa adipocyte complement-related protein antibody
- ACDC antibody
see all
Images
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Western blot - Anti-Adiponectin antibody [EPR17019] (ab181281)All lanes : Anti-Adiponectin antibody [EPR17019] (ab181281) at 1/1000 dilution
Lane 1 : Mouse plasma lysate
Lane 2 : Mouse serum lysate
Lane 3 : Mouse placenta lysate
Lane 4 : Mouse white adipose lysate
Lane 5 : Mouse kidney lysate
Lane 6 : Mouse liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 26 kDa
Observed band size: 26 kDaExposure times: Lanes 1-2,4-5: 1 second; Lane 3: 3 minutes; Lane 6: 3 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Immunocytochemistry/ Immunofluorescence - Anti-Adiponectin antibody [EPR17019] (ab181281)](https://www.abcam.com/ps/products/181/ab181281/Images/ab181281-288744-anti-adiponectin-antibody-epr17019-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Adiponectin antibody [EPR17019] (ab181281)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized 3T3-L1 (mouse embryonic fibroblast cell line) cells labeling Adiponectin with ab181281 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on 3T3-L1 cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adiponectin antibody [EPR17019] (ab181281)](https://www.abcam.com/ps/products/181/ab181281/Images/ab181281-288746-anti-adiponectin-antibody-epr17019-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adiponectin antibody [EPR17019] (ab181281)Immunohistochemical analysis of paraffin-embedded mouse adipose tissue labeling Adiponectin with ab181281 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on mouse adipocytes (PMID: 25676879). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Flow Cytometry (Intracellular) - Anti-Adiponectin antibody [EPR17019] (ab181281)](https://www.abcam.com/ps/products/181/ab181281/Images/ab181281-288743-anti-adiponectin-antibody-epr17019-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Adiponectin antibody [EPR17019] (ab181281)Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized 3T3-L1 (mouse embryonic fibroblast cell line) cell line labeling Adiponectin with ab181281 at 1/50 (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adiponectin antibody [EPR17019] (ab181281)](https://www.abcam.com/ps/products/181/ab181281/Images/ab181281-288745-anti-adiponectin-antibody-epr17019-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adiponectin antibody [EPR17019] (ab181281)Immunohistochemical analysis of paraffin-embedded rat adipose tissue labeling Adiponectin with ab181281 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on rat adipocytes (PMID: 25676879). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Anti-Adiponectin antibody [EPR17019] (ab181281)](https://www.abcam.com/ps/products/181/ab181281/Images/ab181281-6-benefits-of-recombinant-antibodies.png)
Anti-Adiponectin antibody [EPR17019] (ab181281)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab181281 has been referenced in 2 publications.
- Maldonado M et al. The consequences of a high-calorie diet background before calorie restriction on skeletal muscles in a mouse model. Aging (Albany NY) 13:16834-16858 (2021). PubMed: 34166224
- Shang GK et al. Pim1 knockout alleviates sarcopenia in aging mice via reducing adipogenic differentiation of PDGFRa+ mesenchymal progenitors. J Cachexia Sarcopenia Muscle N/A:N/A (2021). PubMed: 34435457