Recombinant
RabMAb

Recombinant Anti-Adiponectin antibody [EPR17019] (ab181281)

Submit a review Submit a question

Overview

  • Product name

    Anti-Adiponectin antibody [EPR17019]
    See all Adiponectin primary antibodies

  • Description

    Rabbit monoclonal [EPR17019] to Adiponectin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment within Mouse Adiponectin aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q60994

  • Positive control

    • WB: Mouse plasma, serum, placenta, white adipose, kidney and liver lysates. IHC-P: Mouse and rat adipose tissues. ICC/IF: 3T3-L1 cells. Flow Cyt: 3T3-L1 cells.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).

We don’t recommend WB for rat species because we observed an extra band around 24 kDa in addition to adiponectin, in rat plasma and rat serum lysates.
ICC/IF 1/1000.
Flow Cyt 1/50.

Target

  • Function

    Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.

  • Tissue specificity

    Synthesized exclusively by adipocytes and secreted into plasma.

  • Involvement in disease

    Defects in ADIPOQ are the cause of adiponectin deficiency (ADPND) [MIM:612556]. ADPND results in very low concentrations of plasma adiponectin.
    Genetic variations in ADIPOQ are associated with non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.

  • Sequence similarities

    Contains 1 C1q domain.
    Contains 1 collagen-like domain.

  • Domain

    The C1q domain is commonly called the globular domain.

  • Post-translational
    modifications

    Hydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting.
    HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes.
    O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • 30 kDa adipocyte complement related protein antibody
      • 30 kDa adipocyte complement-related protein antibody
      • ACDC antibody
      • Acrp 30 antibody
      • ACRP30 antibody
      • ADIPO_HUMAN antibody
      • Adipocyte antibody
      • Adipocyte C1q and collagen domain containing protein antibody
      • Adipocyte complement related 30 kDa protein antibody
      • Adipocyte complement related protein of 30 kDa antibody
      • Adipocyte complement-related 30 kDa protein antibody
      • adipocyte-specific secretory protein antibody
      • Adiponectin antibody
      • Adiponectin precursor antibody
      • adiponectin, C1Q and collagen domain containing antibody
      • Adipoq antibody
      • Adipose most abundant gene transcript 1 antibody
      • Adipose most abundant gene transcript 1 protein antibody
      • Adipose specific collagen like factor antibody
      • ADIPQTL1 antibody
      • ADPN antibody
      • APM 1 antibody
      • apM-1 antibody
      • APM1 antibody
      • C1q and collagen domain-containing protein antibody
      • GBP 28 antibody
      • GBP28 antibody
      • Gelatin binding protein antibody
      • Gelatin binding protein 28 antibody
      • gelatin-binding protein 28 antibody
      • Gelatin-binding protein antibody
      • OTTHUMP00000210047 antibody
      see all

    Images

    • Western blot - Anti-Adiponectin antibody [EPR17019] (ab181281)
      Western blot - Anti-Adiponectin antibody [EPR17019] (ab181281)
      All lanes : Anti-Adiponectin antibody [EPR17019] (ab181281) at 1/1000 dilution

      Lane 1 : Mouse plasma lysate
      Lane 2 : Mouse serum lysate
      Lane 3 : Mouse placenta lysate
      Lane 4 : Mouse white adipose lysate
      Lane 5 : Mouse kidney lysate
      Lane 6 : Mouse liver lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Predicted band size: 26 kDa
      Observed band size: 26 kDa



       

      Exposure times: Lanes 1-2,4-5: 1 second; Lane 3: 3 minutes; Lane 6: 3 seconds.

      Blocking/Dilution buffer: 5% NFDM/TBST.

       

    • Immunocytochemistry/ Immunofluorescence - Anti-Adiponectin antibody [EPR17019] (ab181281)
      Immunocytochemistry/ Immunofluorescence - Anti-Adiponectin antibody [EPR17019] (ab181281)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized 3T3-L1 (mouse embryonic fibroblast cell line) cells labeling Adiponectin with ab181281 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on 3T3-L1 cell line.

      The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adiponectin antibody [EPR17019] (ab181281)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adiponectin antibody [EPR17019] (ab181281)

      Immunohistochemical analysis of paraffin-embedded mouse adipose tissue labeling Adiponectin with ab181281 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on mouse adipocytes (PMID: 25676879). Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Flow Cytometry - Anti-Adiponectin antibody [EPR17019] (ab181281)
      Flow Cytometry - Anti-Adiponectin antibody [EPR17019] (ab181281)

      Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized 3T3-L1 (mouse embryonic fibroblast cell line) cell line labeling Adiponectin with ab181281 at 1/50 (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adiponectin antibody [EPR17019] (ab181281)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adiponectin antibody [EPR17019] (ab181281)

      Immunohistochemical analysis of paraffin-embedded rat adipose tissue labeling Adiponectin with ab181281 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on rat adipocytes (PMID: 25676879). Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    References

    ab181281 has not yet been referenced specifically in any publications.

    Publishing research using ab181281? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab181281.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com