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  1. Link

    adiponectin-antibody-epr3218-ab133347.pdf

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RecombinantRabMAb

Recombinant Anti-Adiponectin antibody [EPR3218] (ab133347)

  • Datasheet
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Western blot - Anti-Adiponectin antibody [EPR3218] (ab133347)
  • Western blot - Anti-Adiponectin antibody [EPR3218] (ab133347)
  • Western blot - Anti-Adiponectin antibody [EPR3218] (ab133347)
  • Western blot - Anti-Adiponectin antibody [EPR3218] (ab133347)
  • Anti-Adiponectin antibody [EPR3218] (ab133347)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3218] to Adiponectin
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-Adiponectin antibody [EPR3218]
    See all Adiponectin primary antibodies
  • Description

    Rabbit monoclonal [EPR3218] to Adiponectin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fat, placenta and plasma lysates. HUH7, Human adipose and plasma lysate. Mouse white adipose and plasma lysate. Rat white adipose and plasma lysate. Rat-1 whole cell lysate
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3218
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Adipose Related
    • Acrp
    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Neuroscience
    • Neurology process
    • Metabolism
    • Stem Cells
    • Mesenchymal Stem Cells
    • Adipogenesis
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Diabetes
    • Metabolism
    • Types of disease
    • Obesity
    • Metabolism
    • Types of disease
    • Heart disease
    • Metabolism
    • Types of disease
    • Metabolic disorders

Associated products

  • Alternative Versions

    • Anti-Adiponectin antibody [EPR3218] - BSA and Azide free (ab226122)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Adiponectin protein (ab51294)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
Application notes
Is unsuitable for Flow Cyt,ICC,IHC-P or IP.

Target

  • Function

    Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
  • Tissue specificity

    Synthesized exclusively by adipocytes and secreted into plasma.
  • Involvement in disease

    Defects in ADIPOQ are the cause of adiponectin deficiency (ADPND) [MIM:612556]. ADPND results in very low concentrations of plasma adiponectin.
    Genetic variations in ADIPOQ are associated with non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
  • Sequence similarities

    Contains 1 C1q domain.
    Contains 1 collagen-like domain.
  • Domain

    The C1q domain is commonly called the globular domain.
  • Post-translational
    modifications

    Hydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting.
    HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes.
    O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession Q15848 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9370 Human
    • Entrez Gene: 11450 Mouse
    • Entrez Gene: 246253 Rat
    • Omim: 605441 Human
    • SwissProt: Q15848 Human
    • SwissProt: Q60994 Mouse
    • Unigene: 80485 Human
    • Unigene: 3969 Mouse
    • Alternative names

      • 30 kDa adipocyte complement related protein antibody
      • 30 kDa adipocyte complement-related protein antibody
      • ACDC antibody
      • Acrp 30 antibody
      • ACRP30 antibody
      • ADIPO_HUMAN antibody
      • Adipocyte antibody
      • Adipocyte C1q and collagen domain containing protein antibody
      • Adipocyte complement related 30 kDa protein antibody
      • Adipocyte complement related protein of 30 kDa antibody
      • Adipocyte complement-related 30 kDa protein antibody
      • adipocyte-specific secretory protein antibody
      • Adiponectin antibody
      • Adiponectin precursor antibody
      • adiponectin, C1Q and collagen domain containing antibody
      • Adipoq antibody
      • Adipose most abundant gene transcript 1 antibody
      • Adipose most abundant gene transcript 1 protein antibody
      • Adipose specific collagen like factor antibody
      • ADIPQTL1 antibody
      • ADPN antibody
      • APM 1 antibody
      • apM-1 antibody
      • APM1 antibody
      • C1q and collagen domain-containing protein antibody
      • GBP 28 antibody
      • GBP28 antibody
      • Gelatin binding protein antibody
      • Gelatin binding protein 28 antibody
      • gelatin-binding protein 28 antibody
      • Gelatin-binding protein antibody
      • OTTHUMP00000210047 antibody
      see all

    Images

    • Western blot - Anti-Adiponectin antibody [EPR3218] (ab133347)
      Western blot - Anti-Adiponectin antibody [EPR3218] (ab133347)
      All lanes : Anti-Adiponectin antibody [EPR3218] (ab133347) at 1/1000 dilution (Purified)

      Lane 1 : Mouse white adipose lysate
      Lane 2 : Mouse plasma lysate
      Lane 3 : Rat-1 (Rat embryonic fibroblast) whole cell lysate
      Lane 4 : Rat white adipose lysate
      Lane 5 : Rat plasma lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 26 kDa
      Observed band size: 30 kDa why is the actual band size different from the predicted?



      The expression profile and molecular weight observed are consistent with what have been described in the literatures (PMID: 15855314, 23967179).

    • Western blot - Anti-Adiponectin antibody [EPR3218] (ab133347)
      Western blot - Anti-Adiponectin antibody [EPR3218] (ab133347)
      All lanes : Anti-Adiponectin antibody [EPR3218] (ab133347) at 1/1000 dilution (Purified)

      Lane 1 : Huh7 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
      Lane 2 : Human adipose lysate
      Lane 3 : Human plasma lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 26 kDa
      Observed band size: 30 kDa why is the actual band size different from the predicted?



      The expression profile and molecular weight observed are consistent with what have been described in the literatures (PMID: 15855314, 23967179).

    • Western blot - Anti-Adiponectin antibody [EPR3218] (ab133347)
      Western blot - Anti-Adiponectin antibody [EPR3218] (ab133347)
      All lanes : Anti-Adiponectin antibody [EPR3218] (ab133347) at 1/1000 dilution

      Lane 1 : Human fat lysate
      Lane 2 : Human placenta lysate
      Lane 3 : Human plasma lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 26 kDa

    • Western blot - Anti-Adiponectin antibody [EPR3218] (ab133347)
      Western blot - Anti-Adiponectin antibody [EPR3218] (ab133347)
      All lanes : Anti-Adiponectin antibody [EPR3218] (ab133347)

      Lane 1 : Human plasma lysate treated with Beta-ME and heat
      Lane 2 : Human plasma lysate treated with heat only
      Lane 3 : Human plasma lysate treated with Beta-ME only
      Lane 4 : Human plasma lysate untreated

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 26 kDa

    • Anti-Adiponectin antibody [EPR3218] (ab133347)
      Anti-Adiponectin antibody [EPR3218] (ab133347)

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (1)

    Publishing research using ab133347? Please let us know so that we can cite the reference in this datasheet.

    ab133347 has been referenced in 1 publication.

    • Peng YJ  et al. Adiponectin and adiponectin receptor 1 overexpression enhance inflammatory bowel disease. J Biomed Sci 25:24 (2018). PubMed: 29540173

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