RecombinantRabMAb

Recombinant Anti-Adiponectin antibody [EPR3218] (ab133347)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3218] to Adiponectin
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Adiponectin antibody [EPR3218]
    See all Adiponectin primary antibodies

  • Description

    Rabbit monoclonal [EPR3218] to Adiponectin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to residues in Human Adiponectin (UniProt Q15848).

  • Positive control

    • WB: Human fat, placenta and plasma lysates. HUH7, Human adipose and plasma lysate. Mouse white adipose and plasma lysate. Rat white adipose and plasma lysate. Rat-1 whole cell lysate

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab133347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.

    • Target

    • Function

      Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.

    • Tissue specificity

      Synthesized exclusively by adipocytes and secreted into plasma.

    • Involvement in disease

      Defects in ADIPOQ are the cause of adiponectin deficiency (ADPND) [MIM:612556]. ADPND results in very low concentrations of plasma adiponectin.
      Genetic variations in ADIPOQ are associated with non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.

    • Sequence similarities

      Contains 1 C1q domain.
      Contains 1 collagen-like domain.

    • Domain

      The C1q domain is commonly called the globular domain.

    • Post-translational
      modifications

      Hydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting.
      HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes.
      O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation.

    • Cellular localization

      Secreted.
    • Information by UniProt

    • Database links

      • Alternative names

        • 30 kDa adipocyte complement related protein antibody
        • 30 kDa adipocyte complement-related protein antibody
        • ACDC antibody
        • Acrp 30 antibody
        • ACRP30 antibody
        • ADIPO_HUMAN antibody
        • Adipocyte antibody
        • Adipocyte C1q and collagen domain containing protein antibody
        • Adipocyte complement related 30 kDa protein antibody
        • Adipocyte complement related protein of 30 kDa antibody
        • Adipocyte complement-related 30 kDa protein antibody
        • adipocyte-specific secretory protein antibody
        • Adiponectin antibody
        • Adiponectin precursor antibody
        • adiponectin, C1Q and collagen domain containing antibody
        • Adipoq antibody
        • Adipose most abundant gene transcript 1 antibody
        • Adipose most abundant gene transcript 1 protein antibody
        • Adipose specific collagen like factor antibody
        • ADIPQTL1 antibody
        • ADPN antibody
        • APM 1 antibody
        • apM-1 antibody
        • APM1 antibody
        • C1q and collagen domain-containing protein antibody
        • GBP 28 antibody
        • GBP28 antibody
        • Gelatin binding protein antibody
        • Gelatin binding protein 28 antibody
        • gelatin-binding protein 28 antibody
        • Gelatin-binding protein antibody
        • OTTHUMP00000210047 antibody
        see all

      Images

      • Western blot - Anti-Adiponectin antibody [EPR3218] (ab133347)
        Western blot - Anti-Adiponectin antibody [EPR3218] (ab133347)
        All lanes : Anti-Adiponectin antibody [EPR3218] (ab133347) at 1/1000 dilution (Purified)

        Lane 1 : Mouse white adipose lysate
        Lane 2 : Mouse plasma lysate
        Lane 3 : Rat-1 (Rat embryonic fibroblast) whole cell lysate
        Lane 4 : Rat white adipose lysate
        Lane 5 : Rat plasma lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

        Predicted band size: 26 kDa
        Observed band size: 30 kDa
        why is the actual band size different from the predicted?



        The expression profile and molecular weight observed are consistent with what have been described in the literatures (PMID: 15855314, 23967179).

      • Western blot - Anti-Adiponectin antibody [EPR3218] (ab133347)
        Western blot - Anti-Adiponectin antibody [EPR3218] (ab133347)
        All lanes : Anti-Adiponectin antibody [EPR3218] (ab133347) at 1/1000 dilution (Purified)

        Lane 1 : Huh7 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
        Lane 2 : Human adipose lysate
        Lane 3 : Human plasma lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

        Predicted band size: 26 kDa
        Observed band size: 30 kDa why is the actual band size different from the predicted?



        The expression profile and molecular weight observed are consistent with what have been described in the literatures (PMID: 15855314, 23967179).

      • Western blot - Anti-Adiponectin antibody [EPR3218] (ab133347)
        Western blot - Anti-Adiponectin antibody [EPR3218] (ab133347)
        All lanes : Anti-Adiponectin antibody [EPR3218] (ab133347) at 1/1000 dilution

        Lane 1 : Human fat lysate
        Lane 2 : Human placenta lysate
        Lane 3 : Human plasma lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 26 kDa

      • Western blot - Anti-Adiponectin antibody [EPR3218] (ab133347)
        Western blot - Anti-Adiponectin antibody [EPR3218] (ab133347)
        All lanes : Anti-Adiponectin antibody [EPR3218] (ab133347)

        Lane 1 : Human plasma lysate treated with Beta-ME and heat
        Lane 2 : Human plasma lysate treated with heat only
        Lane 3 : Human plasma lysate treated with Beta-ME only
        Lane 4 : Human plasma lysate untreated

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 26 kDa

      References (1)

      Publishing research using ab133347? Please let us know so that we can cite the reference in this datasheet.

      ab133347 has been referenced in 1 publication.

      • Peng YJ  et al. Adiponectin and adiponectin receptor 1 overexpression enhance inflammatory bowel disease. J Biomed Sci 25:24 (2018). PubMed: 29540173

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