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This antibody may be used in developing sandwich ELISA assays in combination with antibodies specific to N terminus of ADPR2 Receptor
Our Abpromise guarantee covers the use of ab77612 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 44 kDa.|
Buffer used was: 10 mM KHPO4, 140 mM NaCl, pH 7.2.
Acetone fixed cells.
|Flow Cyt||Use at an assay dependent concentration.
ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.
Ab77612 staining Adiponectin receptor 2 in Human liver tissue sections by Immunohistochemistry. Heat mediated antigen retrieval was performed using citric acid. Samples were fixed with paraformaldehyde and blocked with 10% normal goat serum for 15 minutes at 25°C. Samples were incubated with primary antibody for 12 hours at 4°C. A HRP conjugated goat anti-rabbit IgG was used as a secondary antibody at 10µg/ml.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"