Anti-Adiponectin Receptor 2 antibody (ab77612)

Overview

  • Product name
    Anti-Adiponectin Receptor 2 antibody
    See all Adiponectin Receptor 2 primary antibodies
  • Description
    Goat polyclonal to Adiponectin Receptor 2
  • Host species
    Goat
  • Specificity
    Recognizes Human, Rat, Mouse and Macaca ADPR2 Receptor. May cross react with ADPR1 Receptor.
  • Tested applications
    Suitable for: WB, IHC-P, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Macaque monkey
    Predicted to work with: Chicken, Cow, Pig, Xenopus laevis
  • Immunogen

    Synthetic peptide:

    HFHGVSNLQEFRFMIGGGC

    , corresponding to C terminal amino acids 362-380 of Human Adiponectin Receptor 2

  • Positive control
    • Human liver and human muscle tissue
  • General notes

    This antibody may be used in developing sandwich ELISA assays in combination with antibodies specific to N terminus of ADPR2 Receptor

Properties

Applications

Our Abpromise guarantee covers the use of ab77612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 44 kDa.
IHC-P 1/250.

Buffer used was: 10 mM KHPO4, 140 mM NaCl, pH 7.2.

ICC 1/400.

Acetone fixed cells.

Flow Cyt Use at an assay dependent concentration.

ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Receptor for globular and full-length adiponectin (APM1), an essential hormone secreted by adipocytes that acts as an antidiabetic. Probably involved in metabolic pathways that regulate lipid metabolism such as fatty acid oxidation. Mediates increased AMPK, PPARA ligand activity, fatty acid oxidation and glucose uptake by adiponectin. Has some intermediate-affinity receptor activity for both globular and full-length adiponectin.
  • Tissue specificity
    Highly expressed in skeletal muscle, liver and placenta. Weakly expressed in brain, heart, colon, spleen, kidney, thymus, small intestine, peripheral blood leukocytes and lung.
  • Sequence similarities
    Belongs to the ADIPOR family.
  • Domain
    The N-terminus is known to be cytoplasmic while the C-terminus is known to be extracellular.
  • Cellular localization
    Membrane. Localized to the cell membrane and intracellular organelles.
  • Information by UniProt
  • Database links
  • Alternative names
    • PAQR2 antibody
    • ACDCR2 antibody
    • Adiponectin receptor protein 2 antibody
    • Adipor2 antibody
    • ADR2_HUMAN antibody
    • Progestin and adipoQ receptor family member II antibody
    see all

Images

  • Anti-Adiponectin Receptor 2 antibody (ab77612) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 44 kDa
    Observed band size: 50 kDa (why is the actual band size different from the predicted?)


    Exposure time: 20 minutes
  • Paraffin section of human liver tissue stained with antibody to human ADPR2 receptor C-terminus ab77612 at 1/250 dilution.
  • Paraffin section of human muscle tissue stained with antibody to human ADPR2 receptor C-terminus ab77612 at 1/250 dilution.
  • Ab77612 staining Adiponectin receptor 2 in Human liver tissue sections by Immunohistochemistry. Heat mediated antigen retrieval was performed using citric acid. Samples were fixed with paraformaldehyde and blocked with 10% normal goat serum for 15 minutes at 25°C. Samples were incubated with primary antibody for 12 hours at 4°C.  A HRP conjugated goat anti-rabbit IgG was used as a secondary antibody at 10µg/ml.

    See Abreview

References

This product has been referenced in:
  • Yang W  et al. Betaine attenuates chronic alcohol-induced fatty liver by broadly regulating hepatic lipid metabolism. Mol Med Rep 16:5225-5234 (2017). Read more (PubMed: 28849079) »
  • Liu J  et al. Osmotin Protects H9c2 Cells from Simulated Ischemia-Reperfusion Injury through AdipoR1/PI3K/AKT Signaling Pathway. Front Physiol 8:611 (2017). Read more (PubMed: 28993734) »

See all 6 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (multiple myeloma)
Gel Running Conditions
Reduced Denaturing (4-12% NuPAGE Bis/Tris precast)
Loading amount
30 µg
Specification
multiple myeloma
Blocking step
Roti block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
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Abcam user community

Verified customer

Submitted Jun 15 2018

Thank you for contacting us and your interest in our products.

As you have seen on the datasheets for the anti-Adiponectin Receptor 2 antibody (ab77612) and anti-Adiponectin Receptor 1 antibody [EPR6626] (ab126611) have both been used in flow ...

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In order to help you to choose the correct secondary antibody, could you please indicate which conjugate you would like to haveand which application you use the primary ab77612 in?

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has ca...

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Thank you for your reply. I am sorry that this product is still not meeting your expectations. I would be happy to offer a replacement or credit. Please let me know which you would prefer. Can you please provide an order number or PO so that I may ...

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Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring...

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Application
Western blot
Sample
Pig Cell lysate - whole cell (porcine skeletal muscle)
Loading amount
50 µg
Specification
porcine skeletal muscle
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
RotiBlock (Carl Roth GmbH & Co. KG, Karlsruhe, Germany) in TBS (Tris buffered saline, 200 mM Tris, 1.37 M NaCl, pH 7.4) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
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Abcam user community

Verified customer

Submitted Aug 22 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Liver)
Specification
Liver
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
Normal goat serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 08 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (mesothelioma cell line (ATCC: MSTO-211H))
Specification
mesothelioma cell line (ATCC: MSTO-211H)
Preparation
Cell harvesting/tissue preparation method: 10% FCS / RPMI 1640
Sample buffer: 2% FCS / PBS
Fixation
non-fixation
Permeabilization
No
Gating Strategy
R1 and R2
Username

Abcam user community

Verified customer

Submitted Feb 22 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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