Overview

  • Product name

  • Description

    Rabbit polyclonal to ADIPOR1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow, Pig, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to ADIPOR1 aa 350 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab59184, ab74394)

  • Positive control

    • ICC/IF: HepG2 cells treated with sodium 4-phenylbutyrate. HepG2 cells treated with valproic acid, sodium salt. HeLa and HepG2 cells. WB: Rat, mouse and human skeletal muscle tissue lysate.
  • General notes

     This product was previously labelled as Adiponectin Receptor 1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab70362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).

Target

  • Function

    Receptor for globular and full-length adiponectin (APM1), an essential hormone secreted by adipocytes that acts as an antidiabetic. Probably involved in metabolic pathways that regulate lipid metabolism such as fatty acid oxidation. Mediates increased AMPK, PPARA ligand activity, fatty acid oxidation and glucose uptake by adiponectin. Has some high-affinity receptor for globular adiponectin but low-affinity receptor for full-length adiponectin.
  • Tissue specificity

    Widely expressed. Highly expressed in skeletal muscle. Expressed at intermediate level in brain, heart, spleen, kidney, liver, placenta, lung and peripheral blood leukocytes. Weakly expressed in colon, thymus and small intestine.
  • Sequence similarities

    Belongs to the ADIPOR family.
  • Domain

    The N-terminus is known to be cytoplasmic while the C-terminus is known to be extracellular.
  • Cellular localization

    Membrane. Localized to the cell membrane and intracellular organelles.
  • Information by UniProt
  • Database links

  • Alternative names

    • ACDCR1 antibody
    • ADIPO R1 antibody
    • Adiponectin receptor protein 1 antibody
    • ADIPOR 1 antibody
    • Adipor1 antibody
    • ADR1_HUMAN antibody
    • CGI 45 antibody
    • CGI 45 protein antibody
    • CGI-45 antibody
    • CGI45 antibody
    • CGI45 protein antibody
    • FLJ25385 antibody
    • FLJ42464 antibody
    • PAQR1 antibody
    • Progestin and adipoQ receptor family member I antibody
    • TESBP1A antibody
    see all

Images

  • ab70362 staining ADIPOR1 in HepG2 cells treated with sodium 4-phenylbutyrate (ab141253), by ICC/IF. Increase of aADIPOR1 expression correlates with increased concentration of sodium 4-phenylbutyrate, as described in literature.
    The cells were incubated at 37°C for 6 hours in media containing different concentrations of ab141253 (sodium 4-phenylbutyrate) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab70362 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

  • All lanes : Anti-ADIPOR1 antibody (ab70362) at 1 µg/ml

    Lane 1 : Skeletal Muscle (Rat) Tissue Lysate
    Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 3 : Skeletal Muscle (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 43 kDa
    Observed band size: 43 kDa


    Exposure time: 90 seconds
  • ab70362 staining ADIPOR1 in HepG2 cells treated with valproic acid, sodium salt (ab120745), by ICC/IF. Increased cytoplasmatic staining of ADIPOR1 correlates with increased concentration of valproic acid, as described in literature.
    The cells were incubated at 37øC for 24h in media containing different concentrations of ab120745 (valproic acid) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab70362 (5 μg/ml) was performed overnight at 4øC in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

  • ICC/IF image of ab70362 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70362, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 5µg/ml, and in 4% PFA fixed (10 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.
  • ICC/IF image of ab70362 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70362, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Guo R  et al. Adiponectin and its receptors are involved in hypertensive vascular injury. Mol Med Rep 17:209-215 (2018). Read more (PubMed: 29115432) »
  • Sarmento-Cabral A  et al. Adipokines and Their Receptors Are Widely Expressed and Distinctly Regulated by the Metabolic Environment in the Prostate of Male Mice: Direct Role Under Normal and Tumoral Conditions. Endocrinology 158:3540-3552 (2017). Read more (PubMed: 28938461) »
See all 7 Publications for this product

Customer reviews and Q&As

1-10 of 13 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mammary gland of nursing mouse)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH6
Permeabilization
No
Specification
Mammary gland of nursing mouse
Blocking step
5% BSA 3% horse serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde

Flavia Ghiraldini

Verified customer

Submitted Oct 23 2018

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293)
Permeabilization
Yes - 0.1% Triton X100
Specification
HEK293
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Sep 30 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 5 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
4% paraformaldehyde and 0.1% glutaraldehyde

Abcam user community

Verified customer

Submitted Jun 03 2016

Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE)
Loading amount
30 µg
Specification
Liver
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 23 2014

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Adipose tissue)
Specification
Adipose tissue
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Formaldehyde

Ms. Gina Youcef

Verified customer

Submitted May 28 2014

Answer

I am sorry to read that you were still not able to get a good result. Was this the case for the both of the receptor antibodies? I see my colleague is discussing the adiponectin antibody with you in a separate e-mail exchange.

For an AdipoR2 antibody, I suggest ab113538:

Click here (or use the following: https://www.abcam.com/index.html?datasheet=113538).

Our guarantee is in effect for 6 months following the date of purchase. If you have any questions, or would like a suggestion for other antibodies, please contact me.

Read More

Question
Answer

Thank you for sending your protocol and blot images for this antibody and for ab77612, Anti-Adiponectin Receptor 2.

I think your protocol is correct. Have you tried staining blots of these samples with any other antibodies using this protocol? My concern is about the quality of the samples. Were they harvested with protease inhibitors? If not, there is a possibility that they are degraded. However, you should still see a more prominent signal at the expected molecular weights.

Another consideration is how the samples are prepared before loading into the gel. Membrane receptors have a tendency to aggregate when boiled, and consequently do not enter the gel, so many researchers will heat samples to no hotter than 60-70C. This will denature the sample without causing the membrane proteins to aggregate.

If you are confident that your sample preparation is not the issue , then it may be best to replace the antibodies. Can you please tell me when you ordered them, and the order number?

Read More

Answer

Thank you for contacting Abcam regarding our Anti-Adiponectin Receptor 1 antibodies.


Regarding ab126611, this is a rabbit monoclonal antibody and [EPR6626] represents the clone number.


In terms of the differences between these two antibodies, it is true that ab126611 is a monoclonal and ab70362 is a polyclonal antibody, however some other differences also exist.


ab70362 is validated for ICC/IF and WB in Mouse, Human, and Rat samples. On the other hand, ab126611 is validated for WB, IHC-P, Flow cytometry, and ICC/IF for Mouse, Rat, and Human samples. The purity and buffer compositions are also different, so please keep this in mind when selecting which antibody is most suitable for your experiments.


I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

Read More
Application
Western blot
Sample
Pig Cell lysate - whole cell (porcine skeletal muscle cells)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
50 µg
Specification
porcine skeletal muscle cells
Blocking step
RotiBlock (Carl Roth GmbH & Co. KG, Karlsruhe, Germany) in TBS (Tris buffered saline, 200 mM Tris, 1.37 M NaCl, pH 7.4) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Aug 22 2011

1-10 of 13 Abreviews or Q&A

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