
Recombinant Anti-ADIPOR1 antibody [EPR6626] (Alexa Fluor® 488) (ab200634)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-ADIPOR1 antibody [EPR6626] (Alexa Fluor® 488)
See all ADIPOR1 primary antibodies -
Description
Rabbit monoclonal [EPR6626] to ADIPOR1 (Alexa Fluor® 488) -
Host species
Rabbit -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human ADIPOR1 aa 1-100 (intracellular). The exact sequence is proprietary.
Database link: Q96A54 -
Positive control
- ICC/IF: HeLa cells
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General notes
This product was previously labelled as Adiponectin Receptor 1
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Dissociation constant (KD)
KD = 3.12 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol, 1% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6626 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab200634 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | 1/100. |
Target
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Function
Receptor for globular and full-length adiponectin (APM1), an essential hormone secreted by adipocytes that acts as an antidiabetic. Probably involved in metabolic pathways that regulate lipid metabolism such as fatty acid oxidation. Mediates increased AMPK, PPARA ligand activity, fatty acid oxidation and glucose uptake by adiponectin. Has some high-affinity receptor for globular adiponectin but low-affinity receptor for full-length adiponectin. -
Tissue specificity
Widely expressed. Highly expressed in skeletal muscle. Expressed at intermediate level in brain, heart, spleen, kidney, liver, placenta, lung and peripheral blood leukocytes. Weakly expressed in colon, thymus and small intestine. -
Sequence similarities
Belongs to the ADIPOR family. -
Domain
The N-terminus is known to be cytoplasmic while the C-terminus is known to be extracellular. -
Cellular localization
Membrane. Localized to the cell membrane and intracellular organelles. - Information by UniProt
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Database links
- Entrez Gene: 51094 Human
- Entrez Gene: 72674 Mouse
- Entrez Gene: 289036 Rat
- Omim: 607945 Human
- SwissProt: Q96A54 Human
- SwissProt: Q91VH1 Mouse
- Unigene: 5298 Human
- Unigene: 259976 Mouse
see all -
Alternative names
- ACDCR1 antibody
- ADIPO R1 antibody
- Adiponectin receptor protein 1 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ADIPOR1 antibody [EPR6626] (Alexa Fluor® 488) (ab200634)
ab200634 staining ADIPOR1 in HeLa cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab200634 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 2µg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Datasheets and documents
References
ab200634 has not yet been referenced specifically in any publications.