Recombinant Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free (ab240022)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free
See all ADIPOR1 primary antibodies -
Description
Rabbit monoclonal [EPR6626] to ADIPOR1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, Flow Cyt, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human ADIPOR1 aa 1-100 (intracellular). The exact sequence is proprietary.
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General notes
ab240022 is the carrier-free version of ab126611 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab240022 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as Adiponectin Receptor 1
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Dissociation constant (KD)
KD = 3.12 x 10 -11 M Learn more about KD -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6626 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Immunohistochemistry kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab240022 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| Flow Cyt | Use at an assay dependent concentration. ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| ICC/IF | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 43 kDa. |
Target
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Function
Receptor for globular and full-length adiponectin (APM1), an essential hormone secreted by adipocytes that acts as an antidiabetic. Probably involved in metabolic pathways that regulate lipid metabolism such as fatty acid oxidation. Mediates increased AMPK, PPARA ligand activity, fatty acid oxidation and glucose uptake by adiponectin. Has some high-affinity receptor for globular adiponectin but low-affinity receptor for full-length adiponectin. -
Tissue specificity
Widely expressed. Highly expressed in skeletal muscle. Expressed at intermediate level in brain, heart, spleen, kidney, liver, placenta, lung and peripheral blood leukocytes. Weakly expressed in colon, thymus and small intestine. -
Sequence similarities
Belongs to the ADIPOR family. -
Domain
The N-terminus is known to be cytoplasmic while the C-terminus is known to be extracellular. -
Cellular localization
Membrane. Localized to the cell membrane and intracellular organelles. - Information by UniProt
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Database links
- Entrez Gene: 51094 Human
- Entrez Gene: 72674 Mouse
- Entrez Gene: 289036 Rat
- Omim: 607945 Human
- SwissProt: Q96A54 Human
- SwissProt: Q91VH1 Mouse
- Unigene: 5298 Human
- Unigene: 259976 Mouse
see all -
Alternative names
- ACDCR1 antibody
- ADIPO R1 antibody
- Adiponectin receptor protein 1 antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free (ab240022)](https://www.abcam.com/ps/products/240/ab240022/Images/ab240022-5-ab126611236817126611ICC.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free (ab240022)Immunocytochemistry of HeLa cells fixed in 4% PFA with purified ab126611 at a dilution of 1/400. An Alexa Fluor® goat anti-rabbit antibody was used as the secondary and the sample was counter stained wtih DAPI.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126611).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free (ab240022)](https://www.abcam.com/ps/products/240/ab240022/Images/ab240022-4-ab126611236816126611IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free (ab240022)Immunohistochemical staining of paraffin embedded human endometrium with purified ab126611 at a dilution of 1/200. The seconday was a HRP polymer for rabbit IgG amd the counter stain used was hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126611).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free (ab240022)](https://www.abcam.com/ps/products/240/ab240022/Images/ab240022-3-AdiponectinReceptor1Primaryantibodiesab1266111.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free (ab240022)Immunohistochemical staining of ADIPOR1 in formalin-fixed, paraffin-embedded human kidney tissue by unpurified ab126611, at 1/50 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126611).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Flow Cytometry - Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free (ab240022)](https://www.abcam.com/ps/products/240/ab240022/Images/ab240022-2-AdiponectinReceptor1Primaryantibodiesab1266113.jpg)
Flow Cytometry - Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free (ab240022)Overlay histogram showing HepG2 cells stained with unpurified ab126611 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126611, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) ( 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126611).
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![Other - Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free (ab240022)](https://www.abcam.com/ps/products/240/ab240022/Images/ab240022-1-AdiponectinReceptor1Primaryantibodiesab1266114.jpg)
Other - Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free (ab240022)Equilibrium disassociation constant (KD) with unpurified ab126611.
Learn more about KD
Click here to learn more about KDThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126611).
Datasheets and documents
References
ab240022 has not yet been referenced specifically in any publications.
