RecombinantRabMAb

Recombinant Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free (ab240022)

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Immunocytochemistry/ Immunofluorescence - Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free (ab240022)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free (ab240022)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free (ab240022)
  • Flow Cytometry - Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free (ab240022)
  • OI-RD Scanning - Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free (ab240022)
  • Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free (ab240022)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6626] to ADIPOR1 - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt, ICC/IF, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free
    See all ADIPOR1 primary antibodies

  • Description

    Rabbit monoclonal [EPR6626] to ADIPOR1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Flow Cyt, ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human ADIPOR1 aa 1-100 (intracellular). The exact sequence is proprietary.

  • General notes

    ab240022 is the carrier-free version of ab126611 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab240022 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Adiponectin Receptor 1

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab240022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 43 kDa.

Target

  • Function

    Receptor for globular and full-length adiponectin (APM1), an essential hormone secreted by adipocytes that acts as an antidiabetic. Probably involved in metabolic pathways that regulate lipid metabolism such as fatty acid oxidation. Mediates increased AMPK, PPARA ligand activity, fatty acid oxidation and glucose uptake by adiponectin. Has some high-affinity receptor for globular adiponectin but low-affinity receptor for full-length adiponectin.

  • Tissue specificity

    Widely expressed. Highly expressed in skeletal muscle. Expressed at intermediate level in brain, heart, spleen, kidney, liver, placenta, lung and peripheral blood leukocytes. Weakly expressed in colon, thymus and small intestine.

  • Sequence similarities

    Belongs to the ADIPOR family.

  • Domain

    The N-terminus is known to be cytoplasmic while the C-terminus is known to be extracellular.

  • Cellular localization

    Membrane. Localized to the cell membrane and intracellular organelles.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • ACDCR1 antibody
    • ADIPO R1 antibody
    • Adiponectin receptor protein 1 antibody
    • ADIPOR 1 antibody
    • Adipor1 antibody
    • ADR1_HUMAN antibody
    • CGI 45 antibody
    • CGI 45 protein antibody
    • CGI-45 antibody
    • CGI45 antibody
    • CGI45 protein antibody
    • FLJ25385 antibody
    • FLJ42464 antibody
    • PAQR1 antibody
    • Progestin and adipoQ receptor family member I antibody
    • TESBP1A antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free (ab240022)
    Immunocytochemistry/ Immunofluorescence - Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free (ab240022)

    Immunocytochemistry of HeLa cells fixed in 4% PFA with purified ab126611 at a dilution of 1/400. An Alexa Fluor® goat anti-rabbit antibody was used as the secondary and the sample was counter stained wtih DAPI.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126611).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free (ab240022)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free (ab240022)

    Immunohistochemical staining of paraffin embedded human endometrium with purified ab126611 at a dilution of 1/200. The seconday was a HRP polymer for rabbit IgG amd the counter stain used was hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126611).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free (ab240022)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free (ab240022)

    Immunohistochemical staining of ADIPOR1 in formalin-fixed, paraffin-embedded human kidney tissue by unpurified ab126611, at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126611).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free (ab240022)
    Flow Cytometry - Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free (ab240022)

    Overlay histogram showing HepG2 cells stained with unpurified ab126611 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126611, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) ( 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126611).

  • OI-RD Scanning - Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free (ab240022)
    OI-RD Scanning - Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free (ab240022)

    Equilibrium disassociation constant (KD) with unpurified ab126611.
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126611).

  • Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free (ab240022)
    Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free (ab240022)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab240022? Please let us know so that we can cite the reference in this datasheet.

ab240022 has not yet been referenced specifically in any publications.

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