Key features and details
- Rabbit polyclonal to Adipose Triglyceride Lipase
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Adipose Triglyceride Lipase antibody
See all Adipose Triglyceride Lipase primary antibodies
DescriptionRabbit polyclonal to Adipose Triglyceride Lipase
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Recombinant fragment corresponding to Human Adipose Triglyceride Lipase aa 372-463. Numbering is from isoform 1; the sequence also occus in isoform 2.
Database link: Q96AD5
- A549 cells.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab220738 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionCatalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. Also has acylglycerol transacylase activity. May act coordinately with LIPE/HLS within the lipolytic cascade. Regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion.
Tissue specificityHighest expression in adipose tissue. Also detected in heart, skeletal muscle, and portions of the gastrointestinal tract. Detected in normal retina and retinoblastoma cells. Detected in retinal pigment epithelium and, at lower intensity, in the inner segments of photoreceptors and in the ganglion cell layer of the neural retina (at protein level).
PathwayGlycerolipid metabolism; triacylglycerol degradation.
Involvement in diseaseNote=Genetic variations in PNPLA2 may be associated with risk of diabetes mellitus type 2.
Defects in PNPLA2 are the cause of neutral lipid storage disease with myopathy (NLSDM) [MIM:610717]; also known as neutral lipid storage disease without ichthyosis. NSLDM is a neutral lipid storage disorder (NLSD) with myopathy but without ichthyosis. NLSDs are characterized by the presence of triglyceride-containing cytoplasmic droplets in leukocytes and in other tissues, including bone marrow, skin, and muscle. Individuals with NLSDM did not show obesity, in spite of a defect in triglyceride degradation in fibroblasts and in marked triglyceride storage in liver, muscles, and other visceral cells.
Sequence similaritiesContains 1 patatin domain.
Developmental stageInduced during differentiation of primary preadipocytes to adipocytes. Expression increased from fetal to adult in retinal pigment epithelium.
Cellular localizationLipid droplet. Cell membrane.
- Information by UniProt
- 1110001C14Rik antibody
- Adipose triglyceride lipase antibody
- ATGL antibody
ab220738 has not yet been referenced specifically in any publications.