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RecombinantRabMAb

Recombinant Anti-Adipose Triglyceride Lipase antibody [EPR19650] (ab207799)

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Western blot - Anti-Adipose Triglyceride Lipase antibody [EPR19650] (ab207799)
  • Immunocytochemistry/ Immunofluorescence - Anti-Adipose Triglyceride Lipase antibody [EPR19650] (ab207799)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adipose Triglyceride Lipase antibody [EPR19650] (ab207799)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adipose Triglyceride Lipase antibody [EPR19650] (ab207799)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adipose Triglyceride Lipase antibody [EPR19650] (ab207799)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adipose Triglyceride Lipase antibody [EPR19650] (ab207799)
  • Immunoprecipitation - Anti-Adipose Triglyceride Lipase antibody [EPR19650] (ab207799)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adipose Triglyceride Lipase antibody [EPR19650] (ab207799)
  • Western blot - Anti-Adipose Triglyceride Lipase antibody [EPR19650] (ab207799)
  • Anti-Adipose Triglyceride Lipase antibody [EPR19650] (ab207799)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19650] to Adipose Triglyceride Lipase
  • Suitable for: WB, IHC-P, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Adipose Triglyceride Lipase antibody [EPR19650]
    See all Adipose Triglyceride Lipase primary antibodies
  • Description

    Rabbit monoclonal [EPR19650] to Adipose Triglyceride Lipase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Adipose Triglyceride Lipase aa 300 to the C-terminus. The exact sequence is proprietary.
    Database link: Q96AD5

  • Positive control

    • WB: Human adipose tissue lysate; Adult mouse and rat adipose tissue lysates. IHC-P: Human Adipose tissue; Mouse and rat white and brown adipose tissue. ICC/IF: 3T3-L1 cells. IP: 3T3-L1 differentiated for 6 days whole cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19650
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Lipases
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipases
    • Metabolism
    • Types of disease
    • Obesity
    • Metabolism
    • Types of disease
    • Metabolic disorders

Associated products

  • Alternative Versions

    • Anti-Adipose Triglyceride Lipase antibody [EPR19650] - BSA and Azide free (ab240381)
  • Compatible Secondaries

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Adipose Triglyceride Lipase protein (His tag) (ab226233)
  • Related Products

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
    • Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)
    • Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

Applications

Our Abpromise guarantee covers the use of ab207799 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).

Abcam recommends milk blocking for this product.

IHC-P 1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/500.
IP 1/30.

Target

  • Function

    Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. Also has acylglycerol transacylase activity. May act coordinately with LIPE/HLS within the lipolytic cascade. Regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion.
  • Tissue specificity

    Highest expression in adipose tissue. Also detected in heart, skeletal muscle, and portions of the gastrointestinal tract. Detected in normal retina and retinoblastoma cells. Detected in retinal pigment epithelium and, at lower intensity, in the inner segments of photoreceptors and in the ganglion cell layer of the neural retina (at protein level).
  • Pathway

    Glycerolipid metabolism; triacylglycerol degradation.
  • Involvement in disease

    Note=Genetic variations in PNPLA2 may be associated with risk of diabetes mellitus type 2.
    Defects in PNPLA2 are the cause of neutral lipid storage disease with myopathy (NLSDM) [MIM:610717]; also known as neutral lipid storage disease without ichthyosis. NSLDM is a neutral lipid storage disorder (NLSD) with myopathy but without ichthyosis. NLSDs are characterized by the presence of triglyceride-containing cytoplasmic droplets in leukocytes and in other tissues, including bone marrow, skin, and muscle. Individuals with NLSDM did not show obesity, in spite of a defect in triglyceride degradation in fibroblasts and in marked triglyceride storage in liver, muscles, and other visceral cells.
  • Sequence similarities

    Contains 1 patatin domain.
  • Developmental stage

    Induced during differentiation of primary preadipocytes to adipocytes. Expression increased from fetal to adult in retinal pigment epithelium.
  • Cellular localization

    Lipid droplet. Cell membrane.
  • Target information above from: UniProt accession Q96AD5 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 57104 Human
    • Entrez Gene: 66853 Mouse
    • Entrez Gene: 361676 Rat
    • Omim: 609059 Human
    • SwissProt: Q96AD5 Human
    • SwissProt: Q8BJ56 Mouse
    • SwissProt: P0C548 Rat
    • Unigene: 654697 Human
    • Unigene: 29998 Mouse
    • Unigene: 19196 Rat
    see all
  • Alternative names

    • 1110001C14Rik antibody
    • Adipose triglyceride lipase antibody
    • ATGL antibody
    • ATGL DESNUTRIN antibody
    • Calcium independent phospholipase A2 antibody
    • Calcium-independent phospholipase A2 antibody
    • Desnutrin antibody
    • EC 3.1.1.3 antibody
    • FP17548 antibody
    • IPLA2 zeta antibody
    • IPLA2-zeta antibody
    • Mutant patatin like phospholipase domain containing 2 antibody
    • Patatin like phospholipase domain containing 2 antibody
    • PATATIN LIKE PHOSPHOLIPASE DOMAIN CONTAINING PROTEIN 2 antibody
    • Patatin-like phospholipase domain-containing protein 2 antibody
    • PEDF R antibody
    • PHOSPHOLIPASE A2 CALCIUM INDEPENDENT ZETA antibody
    • Pigment epithelium derived factor antibody
    • Pigment epithelium-derived factor antibody
    • plpl antibody
    • plpl2 antibody
    • PLPL2_HUMAN antibody
    • Pnpla2 antibody
    • Transport secretion protein 2 antibody
    • Transport secretion protein 2.2 antibody
    • Transport-secretion protein 2 antibody
    • Triglyceride hydrolase antibody
    • TTS 2.2 antibody
    • TTS2 antibody
    • TTS2.2 antibody
    • ZETA antibody
    see all

Images

  • Western blot - Anti-Adipose Triglyceride Lipase antibody [EPR19650] (ab207799)
    Western blot - Anti-Adipose Triglyceride Lipase antibody [EPR19650] (ab207799)
    Lane 1 : Anti-Adipose Triglyceride Lipase antibody [EPR19650] (ab207799) at 1/1000 dilution (2% Bovine Serum Albumin)
    Lane 2 : Anti-Adipose Triglyceride Lipase antibody [EPR19650] (ab207799) at 1/1000 dilution (3% Milk)

    All lanes : Human adipose normal tissue lysate - total protein (ab28980)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 55 kDa
    Observed band size: 55 kDa


    Exposure time: 8 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin (lane 1) and 3% Milk (lane 2) before being incubated with ab207799 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • Immunocytochemistry/ Immunofluorescence - Anti-Adipose Triglyceride Lipase antibody [EPR19650] (ab207799)
    Immunocytochemistry/ Immunofluorescence - Anti-Adipose Triglyceride Lipase antibody [EPR19650] (ab207799)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized 3T3-L1 (mouse embryonic fibroblast cell line) undifferentiated and differentiated cells labeling Adipose Triglyceride Lipase with ab207799 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing positive staining on 3T3-L1 cells differentiated for 6 days. The level of expression in 3T3/L1 can be induced by differentiation treatment according to the literature (PMID 19297333).

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adipose Triglyceride Lipase antibody [EPR19650] (ab207799)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adipose Triglyceride Lipase antibody [EPR19650] (ab207799)

    Immunohistochemical analysis of paraffin-embedded rat brown adipose tissue labeling Adipose Triglyceride Lipase with ab207799 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on rat brown adipose tissue is observed (PMID: 15550674). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adipose Triglyceride Lipase antibody [EPR19650] (ab207799)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adipose Triglyceride Lipase antibody [EPR19650] (ab207799)

    Immunohistochemical analysis of paraffin-embedded rat white adipose tissue labeling Adipose Triglyceride Lipase with ab207799 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on rat white adipose tissue is observed (PMID: 15550674). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adipose Triglyceride Lipase antibody [EPR19650] (ab207799)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adipose Triglyceride Lipase antibody [EPR19650] (ab207799)

    Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue labeling Adipose Triglyceride Lipase with ab207799 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on mouse brown adipose tissue is observed (PMID: 15550674). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adipose Triglyceride Lipase antibody [EPR19650] (ab207799)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adipose Triglyceride Lipase antibody [EPR19650] (ab207799)

    Immunohistochemical analysis of paraffin-embedded mouse white adipose tissue labeling Adipose Triglyceride Lipase with ab207799 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on mouse white adipose tissue is observed (PMID: 15550674). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-Adipose Triglyceride Lipase antibody [EPR19650] (ab207799)
    Immunoprecipitation - Anti-Adipose Triglyceride Lipase antibody [EPR19650] (ab207799)

    Adipose Triglyceride Lipase was immunoprecipitated from 0.35 mg of 3T3-L1 (mouse embryonic fibroblast cell line) differentiated for 6 days whole cell lysate with ab207799 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab207799 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.


    Lane 1: 3T3-L1 differentiated for 6 days whole cell lysate 10 µg (Input).

    Lane 2: ab207799 IP in 3T3-L1 differentiated for 6 days whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207799 in 3T3-L1 differentiated for 6 days whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 1 second.



    All lanes : Anti-Adipose Triglyceride Lipase antibody [EPR19650] (ab207799) at 1/500 dilution

    All lanes :

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Exposure time: 1 second
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adipose Triglyceride Lipase antibody [EPR19650] (ab207799)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adipose Triglyceride Lipase antibody [EPR19650] (ab207799)

    IHC image of Adipose Triglyceride Lipase staining in a formalin-fixed, paraffin-embedded human adipose tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval (EDTA based pH 9.0 solution, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab207799, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. As a negative control (inset), an identical assay was performed without adding the primary antibody.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Western blot - Anti-Adipose Triglyceride Lipase antibody [EPR19650] (ab207799)
    Western blot - Anti-Adipose Triglyceride Lipase antibody [EPR19650] (ab207799)
    All lanes : Anti-Adipose Triglyceride Lipase antibody [EPR19650] (ab207799) at 1/1000 dilution (3% Milk)

    Lane 1 : Adult Mouse Adipose Tissue Lysate
    Lane 2 : Adult Rat Adipose Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 55 kDa
    Observed band size: 55 kDa


    Exposure time: 5 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab207799 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • Anti-Adipose Triglyceride Lipase antibody [EPR19650] (ab207799)
    Anti-Adipose Triglyceride Lipase antibody [EPR19650] (ab207799)

Protocols

  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (1)

    Publishing research using ab207799? Please let us know so that we can cite the reference in this datasheet.

    ab207799 has been referenced in 1 publication.

    • Lim S  et al. Ginsenoside Rb1 Induces Beta 3 Adrenergic Receptor-Dependent Lipolysis and Thermogenesis in 3T3-L1 Adipocytes and db/db Mice. Front Pharmacol 10:1154 (2019). PubMed: 31680950

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