Recombinant Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)] (ab109251)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3444(2)] to Adipose Triglyceride Lipase
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)]
See all Adipose Triglyceride Lipase primary antibodies -
Description
Rabbit monoclonal [EPR3444(2)] to Adipose Triglyceride Lipase -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: 3T3-L1, A431 and A673 cell lysates, human heart, skeletal muscle and adipose tissue lysates. Mouse and rat heart tissue lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3444(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109251 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/1000 - 1/10000.
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Notes |
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WB
1/1000 - 1/10000. |
Target
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Function
Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. Also has acylglycerol transacylase activity. May act coordinately with LIPE/HLS within the lipolytic cascade. Regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion. -
Tissue specificity
Highest expression in adipose tissue. Also detected in heart, skeletal muscle, and portions of the gastrointestinal tract. Detected in normal retina and retinoblastoma cells. Detected in retinal pigment epithelium and, at lower intensity, in the inner segments of photoreceptors and in the ganglion cell layer of the neural retina (at protein level). -
Pathway
Glycerolipid metabolism; triacylglycerol degradation. -
Involvement in disease
Note=Genetic variations in PNPLA2 may be associated with risk of diabetes mellitus type 2.
Defects in PNPLA2 are the cause of neutral lipid storage disease with myopathy (NLSDM) [MIM:610717]; also known as neutral lipid storage disease without ichthyosis. NSLDM is a neutral lipid storage disorder (NLSD) with myopathy but without ichthyosis. NLSDs are characterized by the presence of triglyceride-containing cytoplasmic droplets in leukocytes and in other tissues, including bone marrow, skin, and muscle. Individuals with NLSDM did not show obesity, in spite of a defect in triglyceride degradation in fibroblasts and in marked triglyceride storage in liver, muscles, and other visceral cells. -
Sequence similarities
Contains 1 patatin domain. -
Developmental stage
Induced during differentiation of primary preadipocytes to adipocytes. Expression increased from fetal to adult in retinal pigment epithelium. -
Cellular localization
Lipid droplet. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 57104 Human
- Entrez Gene: 66853 Mouse
- Entrez Gene: 361676 Rat
- Omim: 609059 Human
- SwissProt: Q96AD5 Human
- SwissProt: Q8BJ56 Mouse
- SwissProt: P0C548 Rat
- Unigene: 654697 Human
see all -
Alternative names
- 1110001C14Rik antibody
- Adipose triglyceride lipase antibody
- ATGL antibody
see all
Images
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All lanes : Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)] (ab109251) at 1/5000 dilution (purified)
Lane 1 : Mouse heart tissue lysate
Lane 2 : Rat heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG (specific to the non-reduced form of IgG) at 1/1000 dilution
Observed band size: 54 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM /TBST.
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All lanes : Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)] (ab109251) at 1/1000 dilution (purified)
Lane 1 : Human heart tissue lysate
Lane 2 : A431 whole cell lysate
Lane 3 : A673 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG (specific to the non-reduced form of IgG) at 1/1000 dilution
Observed band size: 54 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM /TBST.
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All lanes : Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)] (ab109251) at 1/1000 dilution (unpurified)
Lane 1 : 3T3-L1 lysate
Lane 2 : A431 lysate
Lane 3 : A673 lysate
Lane 4 : Human heart tissue lysate
Lane 5 : Human skeletal muscle tissue lysate
Lane 6 : Human adipose tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Observed band size: 54 kDa why is the actual band size different from the predicted? -
All lanes : Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)] (ab109251) at 1/1000 dilution
Lane 1 : GST tagged Recombinant Human Adipose Triglyceride Lipase (PNPLA2) protein (Full length, 82 KDa)
Lane 2 : GST tagged Recombinant Human KLF4 protein (Full length, 81 KDa)
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 82 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM/TBST
Gel type: 4-20% gradient gel (SDS-PAGE)
Datasheets and documents
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SDS download
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Datasheet download
References (42)
ab109251 has been referenced in 42 publications.
- Chen Y et al. PP1A prevents ROS-induced pyroptosis by inhibiting MAPK/caspase-3 in mouse adipose tissue. FEBS J 289:3839-3853 (2022). PubMed: 35080339
- Chen L et al. Remodeling of dermal adipose tissue alleviates cutaneous toxicity induced by anti-EGFR therapy. Elife 11:N/A (2022). PubMed: 35324426
- Luo P et al. S-Allylmercaptocysteine improves alcoholic liver disease partly through a direct modulation of insulin receptor signaling. Acta Pharm Sin B 11:668-679 (2021). PubMed: 33777674
- Mendham AE et al. Exercise training improves mitochondrial respiration and is associated with an altered intramuscular phospholipid signature in women with obesity. Diabetologia N/A:N/A (2021). PubMed: 33770195
- Li C et al. Mutation of the novel acetylation site at K414R of BECN1 is involved in adipocyte differentiation and lipolysis. J Cell Mol Med 25:6855-6863 (2021). PubMed: 34085745