Key features and details
- Rabbit polyclonal to Adipose Triglyceride Lipase (phospho S406)
- Suitable for: ELISA, WB
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-Adipose Triglyceride Lipase (phospho S406) antibody
See all Adipose Triglyceride Lipase primary antibodies
DescriptionRabbit polyclonal to Adipose Triglyceride Lipase (phospho S406)
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Dog, Pig, Chinese hamster
- This antibody gave a positive signal in Mouse Brown Adipose Tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab135093 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 55 kDa.Can be blocked with Mouse Adipose Triglyceride Lipase (phospho S406) peptide (ab174058).|
FunctionCatalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. Also has acylglycerol transacylase activity. May act coordinately with LIPE/HLS within the lipolytic cascade. Regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion.
Tissue specificityHighest expression in adipose tissue. Also detected in heart, skeletal muscle, and portions of the gastrointestinal tract. Detected in normal retina and retinoblastoma cells. Detected in retinal pigment epithelium and, at lower intensity, in the inner segments of photoreceptors and in the ganglion cell layer of the neural retina (at protein level).
PathwayGlycerolipid metabolism; triacylglycerol degradation.
Involvement in diseaseNote=Genetic variations in PNPLA2 may be associated with risk of diabetes mellitus type 2.
Defects in PNPLA2 are the cause of neutral lipid storage disease with myopathy (NLSDM) [MIM:610717]; also known as neutral lipid storage disease without ichthyosis. NSLDM is a neutral lipid storage disorder (NLSD) with myopathy but without ichthyosis. NLSDs are characterized by the presence of triglyceride-containing cytoplasmic droplets in leukocytes and in other tissues, including bone marrow, skin, and muscle. Individuals with NLSDM did not show obesity, in spite of a defect in triglyceride degradation in fibroblasts and in marked triglyceride storage in liver, muscles, and other visceral cells.
Sequence similaritiesContains 1 patatin domain.
Developmental stageInduced during differentiation of primary preadipocytes to adipocytes. Expression increased from fetal to adult in retinal pigment epithelium.
Cellular localizationLipid droplet. Cell membrane.
- Information by UniProt
- 1110001C14Rik antibody
- Adipose triglyceride lipase antibody
- ATGL antibody
All lanes : Anti-Adipose Triglyceride Lipase (phospho S406) antibody (ab135093) at 1 µg/ml
Lane 1 : Mouse Brown Adipose Tissue (BAT)
Lane 2 : Mouse Brown Adipose Tissue (BAT) with Mouse Patatin-like phospholipase domain-containing protein 2 Synth (402 - 412) (Phosphorylation S 406) at 1 µg/ml
Lane 3 : Mouse Brown Adipose Tissue (BAT) with Mouse Patatin-like phospholipase domain-containing protein 2 Synth (402 - 413) (No Modifications) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Additional bands at: 35 kDa (possible non-specific binding), 85 kDa (possible non-specific binding)
Exposure time: 20 minutes
ab135093 was tested using an Indirect ELISA approach. The wells were coated with peptide (1µg/ml at 100µl/well) overnight at 4°C, followed by a 5% BSA blocking step for 1 hour at room temperature. The primary Ab was then added at a dilution range of 1- 0.00025µg/ml (100µl/well) for 1hr at room temperature. A HRP-conjugated anti-rabbit IgG (heavy and light chain) was used as a secondary antibody at 1:20,000 dilution for 1hr at room temperature.
ab135093 has been referenced in 10 publications.
- Yin C et al. PID1 alters the antilipolytic action of insulin and increases lipolysis via inhibition of AKT/PKA pathway activation. PLoS One 14:e0214606 (2019). PubMed: 30990811
- Zhou J et al. A Liver-Specific Thyromimetic, VK2809, Decreases Hepatosteatosis in Glycogen Storage Disease Type Ia. Thyroid 29:1158-1167 (2019). PubMed: 31337282
- Cao Q et al. Carotid baroreceptor stimulation in obese rats affects white and brown adipose tissues differently in metabolic protection. J Lipid Res 60:1212-1224 (2019). PubMed: 31126973
- Zhou J et al. Thyroid Hormone Status Regulates Skeletal Muscle Response to Chronic Motor Nerve Stimulation. Front Physiol 10:1363 (2019). PubMed: 31736784
- Bertholdt L et al. Impact of training state on fasting-induced regulation of adipose tissue metabolism in humans. J Appl Physiol (1985) 124:729-740 (2018). PubMed: 29191981
- Snook LA et al. Prior Endurance Training Enhances Beta-Adrenergic Signaling in Epidydimal Adipose from Mice Fed a High-Fat Diet. Obesity (Silver Spring) 25:1699-1706 (2017). PubMed: 28857453
- Iannucci LF et al. Metabolomic analysis shows differential hepatic effects of T2 and T3 in rats after short-term feeding with high fat diet. Sci Rep 7:2023 (2017). PubMed: 28515456
- Liu G et al. ColXV promotes adipocyte differentiation via inhibiting DNA methylation and cAMP/PKA pathway in mice. Oncotarget 8:60135-60148 (2017). PubMed: 28947959
- Chouchani ET et al. Mitochondrial ROS regulate thermogenic energy expenditure and sulfenylation of UCP1. Nature 532:112-6 (2016). PubMed: 27027295
- Kim SJ et al. AMPK Phosphorylates Desnutrin/ATGL and Hormone-Sensitive Lipase To Regulate Lipolysis and Fatty Acid Oxidation within Adipose Tissue. Mol Cell Biol 36:1961-76 (2016). PubMed: 27185873