Overview

  • Product name

  • Description

    Rabbit polyclonal to ADK
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human ADK (internal sequence). The exact sequence is proprietary.
    Database link: P55263

  • Positive control

    • WB: Mouse and rat liver lysates; A431 and HepG2 whole cell lysate (ab7900). IHC-P: Mouse liver and kidney tisue. ICC/IF: A431 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab227087 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 41 kDa.
ICC/IF 1/100 - 1/1000.

Target

Images

  • Anti-ADK antibody (ab227087) at 1/1000 dilution + Mouse liver lysate at 50 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 41 kDa



    10% SDS-PAGE gel.

  • Anti-ADK antibody (ab227087) at 1/1000 dilution + Rat liver lysate at 50 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 41 kDa



    10% SDS-PAGE gel.

  • All lanes : Anti-ADK antibody (ab227087) at 1/1000 dilution

    Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 41 kDa



    10% SDS-PAGE gel.

  • Paraffin-embedded mouse liver tissue stained for ADK with ab227087 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded mouse kidney tissue stained for ADK with ab227087 at 1/500 dilution in immunohistochemical analysis.

  • Paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for ADK (green) using ab227087 at 1/200 dilution in ICC/IF.

    Lower panel costained with Hoechst 33342.

References

ab227087 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab227087.
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