Product nameAnti-ADK antibody - C-terminal
See all ADK primary antibodies
DescriptionRabbit polyclonal to ADK - C-terminal
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Chicken, Cow, Dog, Pig, Chimpanzee, Zebrafish, Orangutan, Xenopus tropicalis
Synthetic peptide within Human ADK aa 312-362 (C terminal). The exact sequence is proprietary. (NP_006712.2).
Database link: P55263
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226187 was affinity purified using an epitope specific to ADK immobilized on solid support.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab226187 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 40 kDa.|
FunctionATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides.
Tissue specificityWidely expressed. Highest level in placenta, liver, muscle and kidney.
PathwayPurine metabolism; AMP biosynthesis via salvage pathway; AMP from adenosine 3',5'-bisphosphate: step 1/1.
Sequence similaritiesBelongs to the carbohydrate kinase pfkB family.
- Information by UniProt
- 2310026J05Rik antibody
- 5033405D03Rik antibody
- Adenosine 5''-phosphotransferase antibody
All lanes : Anti-ADK antibody - C-terminal (ab226187) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 40 kDa
Exposure time: 3 minutes
ADK was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226187 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226187 at 1 µg/ml.
Lane 1: ab226187 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
ab226187 has not yet been referenced specifically in any publications.