Overview

  • Product name

    Anti-ADK antibody - C-terminal
    See all ADK primary antibodies
  • Description

    Rabbit polyclonal to ADK - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Chicken, Cow, Dog, Pig, Chimpanzee, Zebrafish, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide within Human ADK aa 312-362 (C terminal). The exact sequence is proprietary. (NP_006712.2).
    Database link: P55263

  • Positive control

    • WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab226187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 40 kDa.

Target

Images

  • All lanes : Anti-ADK antibody - C-terminal (ab226187) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 40 kDa


    Exposure time: 3 minutes
  • ADK was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226187 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226187 at 1 µg/ml.

    Lane 1: ab226187 IP in HEK-293T whole cell lysate.

    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection:  Chemiluminescence with exposure time of 30 seconds.

References

ab226187 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Caenorhabditis elegans Tissue lysate - whole (Whole worm)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
10 µg
Treatment
10uL Laemmli buffer with B-mercaptoethanol, 70c for 10 mins
Specification
Whole worm
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.002µg/mL · Temperature: 23°C

Abcam user community

Verified customer

Submitted May 22 2018

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