Key features and details
- Rabbit polyclonal to ADK - C-terminal
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-ADK antibody - C-terminal
See all ADK primary antibodies
DescriptionRabbit polyclonal to ADK - C-terminal
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Chicken, Cow, Dog, Pig, Chimpanzee, Zebrafish, Orangutan, Xenopus tropicalis
Synthetic peptide within Human ADK aa 312-362 (C terminal). The exact sequence is proprietary. (NP_006712.2).
Database link: P55263
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226187 was affinity purified using an epitope specific to ADK immobilized on solid support.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab226187 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 40 kDa.|
FunctionATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides.
Tissue specificityWidely expressed. Highest level in placenta, liver, muscle and kidney.
PathwayPurine metabolism; AMP biosynthesis via salvage pathway; AMP from adenosine 3',5'-bisphosphate: step 1/1.
Sequence similaritiesBelongs to the carbohydrate kinase pfkB family.
- Information by UniProt
- 2310026J05Rik antibody
- 5033405D03Rik antibody
- Adenosine 5''-phosphotransferase antibody
All lanes : Anti-ADK antibody - C-terminal (ab226187) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 40 kDa
Exposure time: 3 minutes
ADK was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226187 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226187 at 1 µg/ml.
Lane 1: ab226187 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226187 has not yet been referenced specifically in any publications.