Anti-ADK antibody - C-terminal (ab226187)
Key features and details
- Rabbit polyclonal to ADK - C-terminal
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ADK antibody - C-terminal
See all ADK primary antibodies -
Description
Rabbit polyclonal to ADK - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Chicken, Cow, Dog, Pig, Chimpanzee, Zebrafish, Orangutan, Xenopus tropicalis
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Immunogen
Synthetic peptide within Human ADK aa 312-362 (C terminal). The exact sequence is proprietary. (NP_006712.2).
Database link: P55263 -
Positive control
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226187 was affinity purified using an epitope specific to ADK immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab226187 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-10 µg/mg of lysate. | |
| WB | 1/2000 - 1/10000. Predicted molecular weight: 40 kDa. |
Target
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Function
ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides. -
Tissue specificity
Widely expressed. Highest level in placenta, liver, muscle and kidney. -
Pathway
Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenosine 3',5'-bisphosphate: step 1/1. -
Sequence similarities
Belongs to the carbohydrate kinase pfkB family. - Information by UniProt
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Database links
- Entrez Gene: 423735 Chicken
- Entrez Gene: 450902 Chimpanzee
- Entrez Gene: 783572 Cow
- Entrez Gene: 479253 Dog
- Entrez Gene: 132 Human
- Entrez Gene: 11534 Mouse
- Entrez Gene: 100460175 Orangutan
- Entrez Gene: 100154626 Pig
see all -
Alternative names
- 2310026J05Rik antibody
- 5033405D03Rik antibody
- Adenosine 5''-phosphotransferase antibody
see all
Images
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Western blot - Anti-ADK antibody - C-terminal (ab226187)All lanes : Anti-ADK antibody - C-terminal (ab226187) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 40 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-ADK antibody - C-terminal (ab226187)ADK was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226187 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226187 at 1 µg/ml.
Lane 1: ab226187 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab226187 has not yet been referenced specifically in any publications.
