Application
Western blot
Sample
Caenorhabditis elegans Tissue lysate - whole (Whole worm)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
10 µg
Treatment
10uL Laemmli buffer with B-mercaptoethanol, 70c for 10 mins
Specification
Whole worm
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.002µg/mL · Temperature: 23°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: Tween TBS
Concentration
0.5 µg/ml
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Detection
Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: NA kDa Non-specific: 200, 70, 40 kDa
Positive control
Tubulin
Additional data
Additional Notes
Protein bands are being sent for protein mass spec. to determine which band corresponds to C. elegans ADK
Abcam user community
Verified customer
Submitted May 22 2018