Overview

  • Product name

    Anti-ADK antibody [EPR23166-143]
    See all ADK primary antibodies
  • Description

    Rabbit monoclonal [EPR23166-143] to ADK
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, Flow Cytmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human ADK aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P55263

  • Positive control

    • WB: HeLa, HepG2, Wild-type HAP1, Hepa1-6, PC-3, C6, RAW264.7, PC-12 and NIH/3T3. Mouse and rat liver lysates. IHC-P: Human tonsil, human liver, mouse cerebrum and rat kidney tissues. FC: PC-3 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Applications

Our Abpromise guarantee covers the use of ab243636 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000.
WB 1/1000. Predicted molecular weight: 41 kDa.
Flow Cyt 1/600.
  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function

      ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides.
    • Tissue specificity

      Widely expressed. Highest level in placenta, liver, muscle and kidney.
    • Pathway

      Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenosine 3',5'-bisphosphate: step 1/1.
    • Sequence similarities

      Belongs to the carbohydrate kinase pfkB family.
    • Information by UniProt
    • Database links

    • Alternative names

      • 2310026J05Rik antibody
      • 5033405D03Rik antibody
      • Adenosine 5''-phosphotransferase antibody
      • Adenosine kinase antibody
      • adk antibody
      • ADK_HUMAN antibody
      • AI255373 antibody
      • AI987814 antibody
      • AK antibody
      • MGC6593 antibody
      • OTTHUMP00000019864 antibody
      • OTTHUMP00000019865 antibody
      see all

    Images

    • All lanes : Anti-ADK antibody [EPR23166-143] (ab243636) at 1/1000 dilution

      Lane 1 : C6 (rat glial tumor glial cell), whole cell lysate
      Lane 2 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
      Lane 3 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
      Lane 4 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
      Lane 5 : Mouse liver lysate
      Lane 6 : Rat liver lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 41 kDa
      Observed band size: 41 kDa



      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      The lower band (38 kDa) in liver lysates represents ADK-short isoform.

      The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 29038540, 30944910).

      Exposure time: Lanes 1-4: 10 seconds; Lanes 5-6: 26 seconds.

    • All lanes : Anti-ADK antibody [EPR23166-143] (ab243636) at 1/1000 dilution

      Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg
      Lane 2 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg
      Lane 3 : Wild-type HAP1 whole cell lysate at 40 µg
      Lane 4 : ADK knockout HAP1 whole cell lysate at 40 µg
      Lane 5 : Hepa1-6 (mouse hepatoma epithelial cell), whole cell lysate at 20 µg
      Lane 6 : PC-3 (human prostate adenocarcinoma epithelial cell), whole cell lysate at 20 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 41 kDa



      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 29038540, 30944910).

    • Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized PC-3 (Human prostate adenocarcinoma epithelial cell) cells labeling ADK with ab243636 at 1\500 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.

    • Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling ADK with ab243636 at 1/1000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear and cytoplasmic staining on rat kidney is observed. The section was incubated with ab243636 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Secondary antibody only control: Ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

    • Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling ADK with ab243636 at 1/1000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear and cytoplasmic staining on mouse cerebrum is observed. The section was incubated with ab243636 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Secondary antibody only control: Ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

    • Immunohistochemical analysis of paraffin-embedded human liver tissue labeling ADK with ab243636 at 1/1000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear and cytoplasmic staining on human liver is observed. The section was incubated with ab243636 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Secondary antibody only control: Ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

    • Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling ADK with ab243636 at 1/1000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear and weak cytoplasmic staining on human tonsil is observed. The section was incubated with ab243636 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Secondary antibody only control: Ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

    References

    ab243636 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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