Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6581] to ADO
- Suitable for: WB
- Reacts with: Human
Product nameAnti-ADO antibody [EPR6581]
See all ADO primary antibodies
DescriptionRabbit monoclonal [EPR6581] to ADO
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
corresponding to Human ADO aa 200-300.
Database link: Q96SZ5
- WB: HeLa, HEK-293, and A-673 whole cell lysates.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab134102 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 28-30 kDa (predicted molecular weight: 29 kDa).|
- Information by UniProt
FormADO adds 2 oxygen atoms to free cysteamine to form hypotaurine in the catalytic reaction: 2-aminoethanethiol + O2 = hypotaurine. Mitochondrial protein predicted by Entrez gene.
- 2 aminoethanethiol dioxygenase antibody
- 2-aminoethanethiol dioxygenase antibody
- ADO 2 aminoethanethiol (cysteamine) dioxygenase antibody
All lanes : Anti-ADO antibody [EPR6581] (ab134102) at 1/1000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
Lane 3 : A-673 (Human muscle Ewing's Sarcoma ) whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 28-30 kDa why is the actual band size different from the predicted?
Blocking/Diluting buffer: 5% NFDM/TBST
ab134102 has not yet been referenced specifically in any publications.