Overview

  • Product name

    ADP Assay Kit (Fluorometric)
    See all ADP kits
  • Detection method

    Fluorescent
  • Sample type

    Cell Lysate, Tissue Lysate
  • Assay type

    Quantitative
  • Sensitivity

    0.05 µM
  • Species reactivity

    Reacts with: Mammals, Other species
  • Product overview

    ADP Assay Kit (Fluorometric) (ab211087) provides a sensitive fluorescence method to detect ADP in cell and tissue lysates. This assay is particularly suitable for detecting ADP in samples that contain reducing substances, which may interfere with oxidase-based assays. In this assay, ADP, in the presence of ADP Enzyme Mix is converted to an intermediate, which reacts with the fluorescent probe to generate a strong stable signal at Ex/Em = 535/587 nm. This kit can detect as low as 10 pmol or 0.05 µM of ADP in samples.

  • Notes

    Adenosine diphosphate (ADP) is an organic molecule that plays a critical role in cellular metabolism and energy transfer reactions. ADP is a product of ATP dephosphorylation and it can be rephosphorylated to ATP. These dephosphorylations and rephosphorylations occur via various phosphorylases and kinases. ADP is stored in platelets and can be released to interact with variety of purinergic receptors. ADP levels regulate several enzymes involved in intermediary metabolism. ADP conversion to ATP primarily occurs within the mitochondrion and chloroplast although several such processes also occur in the cytoplasm.

  • Platform

    Microplate reader

Properties

Images

  • Typical ADP standard calibration curve.

  • Measurement of ADP in Jurkat cell lysates (increasing protein concentration 1–10 µg).

  • Measurement of ADP in Jurkat and HeLa cell lysate (2 µg).

Protocols

References

ab211087 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab211087.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up