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I would like to ask you some questions about ADP/ATP Ratio Assay Kit (Bioluminescent) ab65313.
1- Do I need to add totally 150 ul per well in a 96 well plate ?
2- The protocol suggested that we need to add 50 µl Nucleotide Releasing Buffer to 103-104 adherent cell, I'm wondering how I can change the volume of Buffer when I have 1x106 Beas-2B cells.
3- I would also appreciate if you tell me the price and delivery time.
Asked on Feb 04 2013
1. If you are using adherent cells, than yes, you will get a total volume of 150ul.
2. Will have to ask the lab for this, as it not clear from the protocol if more or less buffer is needed. I will get back to you as soon as I receive an update.
3. Please contact our official distributor in Australia, Sapphire, regarding the price and delivery time.
Answered on Feb 04 2013