Question (76021) | ADP/ATP Ratio Assay Kit (Bioluminescent) (ab65313)

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Question

The protocol for this kit states to read the sample in a luminometer after about 2 minutes. Is it possible to wait longer (eg. 10 minutes), or will the signal fade quickly?

Answer

The signal should be fairly stable as long as the sample is protected from light. But we suggest measuring after 2 mins to make sure that no light is quenched. Waiting for 10 mins is not advisable since some light can be quenched during this time. For the specific samples in question, if there is no significant light by 2 mins, then most likely more sample should be used in the well.

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