Question (76800) | ADP/ATP Ratio Assay Kit (Bioluminescent) (ab65313)

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Question

I would like to use ab65313 kit on drug treated cells next week.

I am requested to represent my results per mg protein.

Does the buffer in which i lyes the cells (the nucleotide releasing buffer) allow performing a bradford assay (I use a sigma kit) after the luminescence reading?

Otherwise, would it allow reading hoechst fluorescence after the fact?

Please advise,





Answer

The assay buffer has high concentration of detergent-hence Bradford assay is not recommended. We advise using the BCA assay for protein measurement when using this kit.
This assay is performed on cell lysates, so it would be difficult to label the cells with Hoechst after the assay.

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