Overview

  • Product name

  • Description

    Goat polyclonal to ADRA1B
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig, Syrian hamster
  • Immunogen

    Recombinant fragment corresponding to Human ADRA1B aa 252-264 (internal sequence) (Cysteine residue). NP_000670.1.
    Sequence:

    C-TLRIHSKNFHEDT


    Database link: P35368

  • Positive control

    • WB: Human cerebellum lysate.
  • General notes

     This product was previously labelled as alpha 1b Adrenergic Receptor

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab223236 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab223236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 57 kDa).

Target

  • Function

    This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine (PE)-stimulated ERK signaling in cardiac myocytes.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA1B sub-subfamily.
  • Cellular localization

    Nucleus membrane. Cell membrane. Location at the nuclear membrane facilitates heterooligomerization and regulates ERK-mediated signaling in cardiac myocytes. signaling in cardiac myocytes. Colocalizes with GNAQ, PLCB1 as well as LAP2 at the nuclear membrane of cardiac myocytes.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADA1B_HUMAN antibody
    • ADRA 1 antibody
    • Adra 1b antibody
    • ADRA1 antibody
    • ADRA1B antibody
    • Adrenergic alpha 1B receptor antibody
    • Adrenergic receptor alpha 1b antibody
    • Adrenoceptor alpha 1B antibody
    • Alpha 1 adrenergic receptor antibody
    • Alpha 1B adrenoceptor antibody
    • Alpha 1B adrenoreceptor antibody
    • Alpha-1B adrenergic receptor antibody
    • Alpha-1B adrenoceptor antibody
    • Alpha-1B adrenoreceptor antibody
    • Alpha1 adrenergic receptor antibody
    • Alpha1B adrenergic receptor antibody
    • Alpha1BAR antibody
    see all

Images

  • Anti-ADRA1B antibody (ab223236) at 0.3 µg/ml + Human cerebellum lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 57 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

References

ab223236 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab223236.
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