Key features and details
- Rabbit polyclonal to ADRA1B
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ADRA1B antibody
See all ADRA1B primary antibodies
DescriptionRabbit polyclonal to ADRA1B
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human ADRA1B (C terminal). (NM_000679; P35368)
(Peptide available as
- Human fetal brain lysates; human heart tissue.
This product was previously labelled as alpha 1b Adrenergic Receptor
FormLyophilized:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab84405 is purified by a peptide affinity column.
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab84405 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||1/200 - 1/1000. Predicted molecular weight: 57 kDa.Can be blocked with Human ADRA1B peptide (ab226906).|
|IHC-P||1/100 - 1/500.|
FunctionThis alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine (PE)-stimulated ERK signaling in cardiac myocytes.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA1B sub-subfamily.
Cellular localizationNucleus membrane. Cell membrane. Location at the nuclear membrane facilitates heterooligomerization and regulates ERK-mediated signaling in cardiac myocytes. signaling in cardiac myocytes. Colocalizes with GNAQ, PLCB1 as well as LAP2 at the nuclear membrane of cardiac myocytes.
- Information by UniProt
- ADA1B_HUMAN antibody
- ADRA 1 antibody
- Adra 1b antibody
ab84405, at a 1/100 dilution, staining ADRA1B in formalin fixed, paraffin embedded human heart tissue by Immunohistochemistry.
Anti-ADRA1B antibody (ab84405) at 1/500 dilution + human fetal brain lysates
Predicted band size: 57 kDa
Observed band size: 57 kDa
ICC/IF image of ab84450 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84450, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab84405 has been referenced in 1 publication.
- Mitrano DA et al. D1-dopamine and a1-adrenergic receptors co-localize in dendrites of the rat prefrontal cortex. Neuroscience 258:90-100 (2014). ICC/IF ; Human . PubMed: 24231738