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  1. Link

    adra1b-antibody-ab84405.pdf

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Neuroscience Neurotransmission Receptors / Channels GPCR Adrenergic Receptors
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Anti-ADRA1B antibody (ab84405)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (2)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRA1B antibody (ab84405)
  • Western blot - Anti-ADRA1B antibody (ab84405)
  • Immunocytochemistry/ Immunofluorescence - Anti-ADRA1B antibody (ab84405)

Key features and details

  • Rabbit polyclonal to ADRA1B
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-ADRA1B antibody
    See all ADRA1B primary antibodies
  • Description

    Rabbit polyclonal to ADRA1B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human ADRA1B (C terminal). (NM_000679; P35368)
    (Peptide available as ab226906)

  • Positive control

    • Human fetal brain lysates; human heart tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Lyophilized:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab84405 is purified by a peptide affinity column.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • Adrenergic Receptors
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Human ADRA1B peptide (ab226906)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab84405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use a concentration of 1 µg/ml.
WB
1/200 - 1/1000. Predicted molecular weight: 57 kDa.Can be blocked with Human ADRA1B peptide (ab226906).
IHC-P (1)
1/100 - 1/500.
Notes
ICC/IF
Use a concentration of 1 µg/ml.
WB
1/200 - 1/1000. Predicted molecular weight: 57 kDa.Can be blocked with Human ADRA1B peptide (ab226906).
IHC-P
1/100 - 1/500.

Target

  • Function

    This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine (PE)-stimulated ERK signaling in cardiac myocytes.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA1B sub-subfamily.
  • Cellular localization

    Nucleus membrane. Cell membrane. Location at the nuclear membrane facilitates heterooligomerization and regulates ERK-mediated signaling in cardiac myocytes. signaling in cardiac myocytes. Colocalizes with GNAQ, PLCB1 as well as LAP2 at the nuclear membrane of cardiac myocytes.
  • Target information above from: UniProt accession P35368 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 147 Human
    • Omim: 104220 Human
    • SwissProt: P35368 Human
    • Unigene: 368632 Human
    • Alternative names

      • ADA1B_HUMAN antibody
      • ADRA 1 antibody
      • Adra 1b antibody
      • ADRA1 antibody
      • ADRA1B antibody
      • Adrenergic alpha 1B receptor antibody
      • Adrenergic receptor alpha 1b antibody
      • Adrenoceptor alpha 1B antibody
      • Alpha 1 adrenergic receptor antibody
      • Alpha 1B adrenoceptor antibody
      • Alpha 1B adrenoreceptor antibody
      • Alpha-1B adrenergic receptor antibody
      • Alpha-1B adrenoceptor antibody
      • Alpha-1B adrenoreceptor antibody
      • Alpha1 adrenergic receptor antibody
      • Alpha1B adrenergic receptor antibody
      • Alpha1BAR antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRA1B antibody (ab84405)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRA1B antibody (ab84405)

      ab84405, at a 1/100 dilution, staining ADRA1B in formalin fixed, paraffin embedded human heart tissue by Immunohistochemistry.

    • Western blot - Anti-ADRA1B antibody (ab84405)
      Western blot - Anti-ADRA1B antibody (ab84405)
      Anti-ADRA1B antibody (ab84405) at 1/500 dilution + human fetal brain lysates

      Predicted band size: 57 kDa
      Observed band size: 57 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-ADRA1B antibody (ab84405)
      Immunocytochemistry/ Immunofluorescence - Anti-ADRA1B antibody (ab84405)
      ICC/IF image of ab84450 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84450, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab84405? Please let us know so that we can cite the reference in this datasheet.

    ab84405 has been referenced in 2 publications.

    • Mitrano DA  et al. a1b-Adrenergic Receptor Localization and Relationship to the D1-Dopamine Receptor in the Rat Nucleus Accumbens. Neuroscience 371:126-137 (2018). PubMed: 29229557
    • Mitrano DA  et al. D1-dopamine and a1-adrenergic receptors co-localize in dendrites of the rat prefrontal cortex. Neuroscience 258:90-100 (2014). ICC/IF ; Human . PubMed: 24231738

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-alpha 1b Adrenergic Receptor antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    (agent) for 5 minute(s) · Concentration: 100% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako High pH
    Sample
    Human Tissue sections (Tendon)
    Specification
    Tendon
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 18 2014

    Question

    Can you  tell me which of the following Ab that I have ordered are discontinued? or renamed? and if there is other comparable Ab in your current product line? 1) Alpha 1 a/c https://www.abcam.com/alpha-1c-Adrenergic-Receptor-antibody-ab12935.html 2) Alpha 1 b https://www.abcam.com/alpha-1b-Adrenergic-Receptor-antibody-ab84405.html 3) Alpha 1 d https://www.abcam.com/alpha-1d-Adrenergic-Receptor-antibody-ab12923.html 4) Alpha 2 c https://www.abcam.com/alpha-2C-Adrenergic-Receptor-antibody-ab13298.html Thanks.

    Read More

    Abcam community

    Verified customer

    Asked on Aug 24 2011

    Answer

    Thank you for your response. Regrettably, ab12923, ab12935 and ab13298 have been discontinued so currently we do no have stock of them in our catalogue. I have conducted a search for your and found some alternatives products for these targets. - alpha 1d Adrenergic Receptor: ab84402 - alpha 1c Adrenergic Receptor: - alpha 2C Adrenergic Receptor: I would advise you please to take a look at the on-line datahseets to see which one would suit you need (i.e species, application). I hope this helps and if I can assist further, please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    Answered on Aug 24 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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