Anti-ADRA1B antibody (ab84405)
Key features and details
- Rabbit polyclonal to ADRA1B
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ADRA1B antibody
See all ADRA1B primary antibodies -
Description
Rabbit polyclonal to ADRA1B -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human ADRA1B (C terminal). (NM_000679; P35368)
(Peptide available asab226906) -
Positive control
- Human fetal brain lysates; human heart tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab84405 is purified by a peptide affinity column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab84405 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 1 µg/ml.
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| WB |
1/200 - 1/1000. Predicted molecular weight: 57 kDa.Can be blocked with Human ADRA1B peptide (ab226906).
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| IHC-P | (1) |
1/100 - 1/500.
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| Notes |
|---|
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ICC/IF
Use a concentration of 1 µg/ml. |
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WB
1/200 - 1/1000. Predicted molecular weight: 57 kDa.Can be blocked with Human ADRA1B peptide (ab226906). |
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IHC-P
1/100 - 1/500. |
Target
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Function
This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine (PE)-stimulated ERK signaling in cardiac myocytes. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA1B sub-subfamily. -
Cellular localization
Nucleus membrane. Cell membrane. Location at the nuclear membrane facilitates heterooligomerization and regulates ERK-mediated signaling in cardiac myocytes. signaling in cardiac myocytes. Colocalizes with GNAQ, PLCB1 as well as LAP2 at the nuclear membrane of cardiac myocytes. - Information by UniProt
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Database links
- Entrez Gene: 147 Human
- Omim: 104220 Human
- SwissProt: P35368 Human
- Unigene: 368632 Human
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Alternative names
- ADA1B_HUMAN antibody
- ADRA 1 antibody
- Adra 1b antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRA1B antibody (ab84405)
ab84405, at a 1/100 dilution, staining ADRA1B in formalin fixed, paraffin embedded human heart tissue by Immunohistochemistry.
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Western blot - Anti-ADRA1B antibody (ab84405)Anti-ADRA1B antibody (ab84405) at 1/500 dilution + human fetal brain lysates
Predicted band size: 57 kDa
Observed band size: 57 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-ADRA1B antibody (ab84405)ICC/IF image of ab84450 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84450, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab84405 has been referenced in 2 publications.
- Mitrano DA et al. a1b-Adrenergic Receptor Localization and Relationship to the D1-Dopamine Receptor in the Rat Nucleus Accumbens. Neuroscience 371:126-137 (2018). PubMed: 29229557
- Mitrano DA et al. D1-dopamine and a1-adrenergic receptors co-localize in dendrites of the rat prefrontal cortex. Neuroscience 258:90-100 (2014). ICC/IF ; Human . PubMed: 24231738