• Product name

  • Description

    Rabbit polyclonal to ADRA1B
  • Host species

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human ADRA1B (C terminal). (NM_000679; P35368)
    (Peptide available as ab226906)

  • Positive control

    • Human fetal brain lysates; human heart tissue.
  • General notes

     This product was previously labelled as alpha 1b Adrenergic Receptor



  • Form

    Lyophilised:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab84405 is purified by a peptide affinity column.
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab84405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/200 - 1/1000. Predicted molecular weight: 57 kDa.Can be blocked with Human ADRA1B peptide (ab226906).
IHC-P 1/100 - 1/500.


  • Function

    This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine (PE)-stimulated ERK signaling in cardiac myocytes.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA1B sub-subfamily.
  • Cellular localization

    Nucleus membrane. Cell membrane. Location at the nuclear membrane facilitates heterooligomerization and regulates ERK-mediated signaling in cardiac myocytes. signaling in cardiac myocytes. Colocalizes with GNAQ, PLCB1 as well as LAP2 at the nuclear membrane of cardiac myocytes.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADA1B_HUMAN antibody
    • ADRA 1 antibody
    • Adra 1b antibody
    • ADRA1 antibody
    • ADRA1B antibody
    • Adrenergic alpha 1B receptor antibody
    • Adrenergic receptor alpha 1b antibody
    • Adrenoceptor alpha 1B antibody
    • Alpha 1 adrenergic receptor antibody
    • Alpha 1B adrenoceptor antibody
    • Alpha 1B adrenoreceptor antibody
    • Alpha-1B adrenergic receptor antibody
    • Alpha-1B adrenoceptor antibody
    • Alpha-1B adrenoreceptor antibody
    • Alpha1 adrenergic receptor antibody
    • Alpha1B adrenergic receptor antibody
    • Alpha1BAR antibody
    see all


  • ab84405, at a 1/100 dilution, staining ADRA1B in formalin fixed, paraffin embedded human heart tissue by Immunohistochemistry.

  • Anti-ADRA1B antibody (ab84405) at 1/500 dilution + human fetal brain lysates

    Predicted band size: 57 kDa
    Observed band size: 57 kDa

  • ICC/IF image of ab84450 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84450, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:

  • Mitrano DA  et al. D1-dopamine and a1-adrenergic receptors co-localize in dendrites of the rat prefrontal cortex. Neuroscience 258:90-100 (2014). ICC/IF ; Human . Read more (PubMed: 24231738) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
(agent) for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako High pH
Human Tissue sections (Tendon)

Abcam user community

Verified customer

Submitted Aug 18 2014


Thank you for your response. Regrettably, ab12923, ab12935 and ab13298 have been discontinued so currently we do no have stock of them in our catalogue. I have conducted a search for your and found some alternatives products for these targets. - alpha 1d Adrenergic Receptor: ab84402 - alpha 1c Adrenergic Receptor: - alpha 2C Adrenergic Receptor: I would advise you please to take a look at the on-line datahseets to see which one would suit you need (i.e species, application). I hope this helps and if I can assist further, please do not hesitate to contact me.

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