Product nameAnti-ADRA1B antibody [EPR10336]
See all ADRA1B primary antibodies
DescriptionRabbit monoclonal [EPR10336] to ADRA1B
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt or ICC/IF
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human ADRA1B aa 150-250. The exact sequence is proprietary.
Database link: P35368
- WB: HeLa, PC-3 and NIH/3T3 cell lysate, human fetal brain lysates, mouse and rat brain lysates, mouse and rat stomach lysates
This product was previously labelled as alpha 1b Adrenergic Receptor
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab169523 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 57 kDa.|
FunctionThis alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine (PE)-stimulated ERK signaling in cardiac myocytes.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA1B sub-subfamily.
Cellular localizationNucleus membrane. Cell membrane. Location at the nuclear membrane facilitates heterooligomerization and regulates ERK-mediated signaling in cardiac myocytes. signaling in cardiac myocytes. Colocalizes with GNAQ, PLCB1 as well as LAP2 at the nuclear membrane of cardiac myocytes.
- Information by UniProt
- ADA1B_HUMAN antibody
- ADRA 1 antibody
- Adra 1b antibody
All lanes : Anti-ADRA1B antibody [EPR10336] (ab169523) at 1/2000 dilution (Purified)
Lane 1 : Human fetal brain lysates
Lane 2 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates
Lane 3 : Mouse brain lysates
Lane 4 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
Lane 5 : Rat brain lysates
Lane 6 : Mouse stomach lysates
Lane 7 : Rat stomach lysates
Lane 8 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa
All lanes : Anti-ADRA1B antibody [EPR10336] (ab169523) at 1/1000 dilution (unpurified)
Lane 1 : Fetal brain lysate
Lane 2 : PC-3 lysate
Lane 3 : HeLa lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 57 kDa
This product has been referenced in:
- Gao X et al. Asymmetrical ligand-induced cross-regulation of chemokine (C-X-C motif) receptor 4 by a1-adrenergic receptors at the heteromeric receptor complex. Sci Rep 8:2730 (2018). Read more (PubMed: 29426850) »
- Tyurin-Kuzmin PA et al. Noradrenaline Sensitivity Is Severely Impaired in Immortalized Adipose-Derived Mesenchymal Stem Cell Line. Int J Mol Sci 19:N/A (2018). Read more (PubMed: 30469522) »