Overview

  • Product name

    Anti-ADRA1B antibody [EPR10336]
    See all ADRA1B primary antibodies
  • Description

    Rabbit monoclonal [EPR10336] to ADRA1B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ADRA1B aa 150-250. The exact sequence is proprietary.
    Database link: P35368

  • Positive control

    • WB: HeLa, PC-3 and NIH/3T3 cell lysate, human fetal brain lysates, mouse and rat brain lysates, mouse and rat stomach lysates
  • General notes

     This product was previously labelled as alpha 1b Adrenergic Receptor

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR10336
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab169523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function

      This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine (PE)-stimulated ERK signaling in cardiac myocytes.
    • Sequence similarities

      Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA1B sub-subfamily.
    • Cellular localization

      Nucleus membrane. Cell membrane. Location at the nuclear membrane facilitates heterooligomerization and regulates ERK-mediated signaling in cardiac myocytes. signaling in cardiac myocytes. Colocalizes with GNAQ, PLCB1 as well as LAP2 at the nuclear membrane of cardiac myocytes.
    • Information by UniProt
    • Database links

    • Alternative names

      • ADA1B_HUMAN antibody
      • ADRA 1 antibody
      • Adra 1b antibody
      • ADRA1 antibody
      • ADRA1B antibody
      • Adrenergic alpha 1B receptor antibody
      • Adrenergic receptor alpha 1b antibody
      • Adrenoceptor alpha 1B antibody
      • Alpha 1 adrenergic receptor antibody
      • Alpha 1B adrenoceptor antibody
      • Alpha 1B adrenoreceptor antibody
      • Alpha-1B adrenergic receptor antibody
      • Alpha-1B adrenoceptor antibody
      • Alpha-1B adrenoreceptor antibody
      • Alpha1 adrenergic receptor antibody
      • Alpha1B adrenergic receptor antibody
      • Alpha1BAR antibody
      see all

    Images

    • All lanes : Anti-ADRA1B antibody [EPR10336] (ab169523) at 1/2000 dilution (Purified)

      Lane 1 : Human fetal brain lysates
      Lane 2 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates
      Lane 3 : Mouse brain lysates
      Lane 4 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
      Lane 5 : Rat brain lysates
      Lane 6 : Mouse stomach lysates
      Lane 7 : Rat stomach lysates
      Lane 8 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 57 kDa
      Observed band size: 57 kDa

    • All lanes : Anti-ADRA1B antibody [EPR10336] (ab169523) at 1/1000 dilution (unpurified)

      Lane 1 : Fetal brain lysate
      Lane 2 : PC-3 lysate
      Lane 3 : HeLa lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 57 kDa

    References

    This product has been referenced in:

    • Gao X  et al. Asymmetrical ligand-induced cross-regulation of chemokine (C-X-C motif) receptor 4 by a1-adrenergic receptors at the heteromeric receptor complex. Sci Rep 8:2730 (2018). Read more (PubMed: 29426850) »
    • Tyurin-Kuzmin PA  et al. Noradrenaline Sensitivity Is Severely Impaired in Immortalized Adipose-Derived Mesenchymal Stem Cell Line. Int J Mol Sci 19:N/A (2018). Read more (PubMed: 30469522) »
    See all 7 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab169523.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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