Recombinant Anti-ADRA1B antibody [EPR10336] (ab169523)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRA1B antibody [EPR10336] (ab169523)](https://www.abcam.com/ps/products/169/ab169523/Images/ab169523-9-anti-adra1b-antibody-epr10336-immunohistochemistry-human-kidney.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRA1B antibody [EPR10336] (ab169523)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10336] to ADRA1B
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-ADRA1B antibody [EPR10336]
See all ADRA1B primary antibodies -
Description
Rabbit monoclonal [EPR10336] to ADRA1B -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human ADRA1B aa 150-250. The exact sequence is proprietary.
Database link: P35368 -
Positive control
- WB: HeLa, PC-3 and NIH/3T3 cell lysate, human fetal brain lysates, mouse and rat brain lysates, mouse and rat stomach lysates; IHC-P: Rat and mouse stomach tissue. Human ovarian carcinoma, breast carcinoma, and kidney tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10336 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab169523 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
|
| Notes |
|---|
|
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 57 kDa. |
Target
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Function
This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine (PE)-stimulated ERK signaling in cardiac myocytes. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA1B sub-subfamily. -
Cellular localization
Nucleus membrane. Cell membrane. Location at the nuclear membrane facilitates heterooligomerization and regulates ERK-mediated signaling in cardiac myocytes. signaling in cardiac myocytes. Colocalizes with GNAQ, PLCB1 as well as LAP2 at the nuclear membrane of cardiac myocytes. - Information by UniProt
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Database links
- Entrez Gene: 147 Human
- Entrez Gene: 11548 Mouse
- Entrez Gene: 24173 Rat
- Omim: 104220 Human
- SwissProt: P35368 Human
- SwissProt: P97717 Mouse
- SwissProt: P15823 Rat
- Unigene: 368632 Human
see all -
Alternative names
- ADA1B_HUMAN antibody
- ADRA 1 antibody
- Adra 1b antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRA1B antibody [EPR10336] (ab169523)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ADRA1B with ab169523 at 1/500 (1.242 µg/mL) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining predominantly on collecting duct of human kidney. The section was incubated with ab169523 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 minutes.
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![Western blot - Anti-ADRA1B antibody [EPR10336] (ab169523)](https://www.abcam.com/ps/products/169/ab169523/Images/ab169523-344780-Anti-adra1b-antibody-EPR10336-westernblot-human-mouse.jpg)
Western blot - Anti-ADRA1B antibody [EPR10336] (ab169523)All lanes : Anti-ADRA1B antibody [EPR10336] (ab169523) at 1/2000 dilution (Purified)
Lane 1 : Human fetal brain lysates
Lane 2 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates
Lane 3 : Mouse brain lysates
Lane 4 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
Lane 5 : Rat brain lysates
Lane 6 : Mouse stomach lysates
Lane 7 : Rat stomach lysates
Lane 8 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRA1B antibody [EPR10336] (ab169523)](https://www.abcam.com/ps/products/169/ab169523/Images/ab169523-8-anti-adra1b-antibody-epr10336-immunohistochemistry-human-ovarian-carcinoma.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRA1B antibody [EPR10336] (ab169523)Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling ADRA1B with ab169523 at 1/500 (1.242 µg/mL) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human ovarian carcinoma. The section was incubated with ab169523 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 minutes.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRA1B antibody [EPR10336] (ab169523)](https://www.abcam.com/ps/products/169/ab169523/Images/ab169523-11-anti-adra1b-antibody-epr10336-immunohistochemistry-rat-stomach.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRA1B antibody [EPR10336] (ab169523)Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling ADRA1B with ab169523 at 1/500 (1.242 µg/mL) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on rat stomach. The section was incubated with ab169523 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 minutes.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRA1B antibody [EPR10336] (ab169523)](https://www.abcam.com/ps/products/169/ab169523/Images/ab169523-10-anti-adra1b-antibody-epr10336-immunohistochemistry-mouse-stomach.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRA1B antibody [EPR10336] (ab169523)Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling ADRA1B with ab169523 at 1/500 (1.242 µg/mL) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse stomach. The section was incubated with ab169523 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 minutes.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRA1B antibody [EPR10336] (ab169523)](https://www.abcam.com/ps/products/169/ab169523/Images/ab169523-7-anti-adra1b-antibody-epr10336-immunohistochemistry-human-breast-carcinoma.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRA1B antibody [EPR10336] (ab169523)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling ADRA1B with ab169523 at 1/500 (1.242 µg/mL) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human breast carcinoma. The section was incubated with ab169523 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 minutes.
-
![Western blot - Anti-ADRA1B antibody [EPR10336] (ab169523)](https://www.abcam.com/ps/products/169/ab169523/Images/ab169523-168122-anti-alpha-1b-adrenergic-receptor-antibody-epr10336-western-blot.jpg)
Western blot - Anti-ADRA1B antibody [EPR10336] (ab169523)All lanes : Anti-ADRA1B antibody [EPR10336] (ab169523) at 1/1000 dilution (unpurified)
Lane 1 : Fetal brain lysate
Lane 2 : PC-3 lysate
Lane 3 : HeLa lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 57 kDa -
![Anti-ADRA1B antibody [EPR10336] (ab169523)](https://www.abcam.com/ps/products/169/ab169523/Images/ab169523-5-benefits-of-recombinant-antibodies.png)
Anti-ADRA1B antibody [EPR10336] (ab169523)
Protocols
Datasheets and documents
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Datasheet download
References (16)
ab169523 has been referenced in 16 publications.
- Gao R et al. Macrophage-derived netrin-1 drives adrenergic nerve-associated lung fibrosis. J Clin Invest 131:N/A (2021). PubMed: 33393489
- He W et al. Alterations in the phosphodiesterase type 5 pathway and oxidative stress correlate with erectile function in spontaneously hypertensive rats. J Cell Mol Med 24:14280-14292 (2020). PubMed: 33118708
- Li Z et al. First small-molecule PROTACs for G protein-coupled receptors: inducing a1A-adrenergic receptor degradation. Acta Pharm Sin B 10:1669-1679 (2020). PubMed: 33088687
- Lee JY et al. Expression of bladder a1-adrenoceptor subtype after relief of partial bladder outlet obstruction in a rat model. Investig Clin Urol 61:297-303 (2020). PubMed: 32377606
- Florent R et al. Bim, Puma and Noxa upregulation by Naftopidil sensitizes ovarian cancer to the BH3-mimetic ABT-737 and the MEK inhibitor Trametinib. Cell Death Dis 11:380 (2020). PubMed: 32424251
