Overview

  • Product name

    Anti-ADRA1B antibody [EPR10336] (HRP)
    See all ADRA1B primary antibodies
  • Description

    Rabbit monoclonal [EPR10336] to ADRA1B (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human ADRA1B. The exact sequence is proprietary.
    Database link: P35368

  • Positive control

    • WB: PC3 and HeLa whole cell lysates; Human Fetal brain tissue lysate. IHC-P: normal rat brain tissue sections
  • General notes

     This product was previously labelled as alpha 1b Adrenergic Receptor

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Proclin
    Constituents: 30% Glycerol, 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR10336
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab202936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/5000. Detects a band of approximately 53 kDa (predicted molecular weight: 57 kDa).

Target

  • Function

    This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine (PE)-stimulated ERK signaling in cardiac myocytes.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA1B sub-subfamily.
  • Cellular localization

    Nucleus membrane. Cell membrane. Location at the nuclear membrane facilitates heterooligomerization and regulates ERK-mediated signaling in cardiac myocytes. signaling in cardiac myocytes. Colocalizes with GNAQ, PLCB1 as well as LAP2 at the nuclear membrane of cardiac myocytes.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADA1B_HUMAN antibody
    • ADRA 1 antibody
    • Adra 1b antibody
    • ADRA1 antibody
    • ADRA1B antibody
    • Adrenergic alpha 1B receptor antibody
    • Adrenergic receptor alpha 1b antibody
    • Adrenoceptor alpha 1B antibody
    • Alpha 1 adrenergic receptor antibody
    • Alpha 1B adrenoceptor antibody
    • Alpha 1B adrenoreceptor antibody
    • Alpha-1B adrenergic receptor antibody
    • Alpha-1B adrenoceptor antibody
    • Alpha-1B adrenoreceptor antibody
    • Alpha1 adrenergic receptor antibody
    • Alpha1B adrenergic receptor antibody
    • Alpha1BAR antibody
    see all

Images

  • IHC image of ADRA1B staining in a section of formalin-fixed paraffin-embedded normal rat brain, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab202936, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-ADRA1B antibody [EPR10336] (HRP) (ab202936) at 1/5000 dilution

    Lane 1 : Brain (Human) Tissue Lysate - fetal normal tissue
    Lane 2 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 57 kDa
    Observed band size: 53 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab202936 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

     

References

ab202936 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab202936.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up