Product nameAnti-ADRA2B antibody [EPR9623]
See all ADRA2B primary antibodies
DescriptionRabbit monoclonal [EPR9623] to ADRA2B
Tested applicationsSuitable for: WB, Flow Cyt, IPmore details
Unsuitable for: ICC/IF or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
- Human fetal brain, MCF7, HepG2 and 293T cell lysates; 293T cells.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab151727 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 50 kDa.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IP||1/10 - 1/100.|
RelevanceAlpha-2-adrenergic receptors are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. Alpha 2B adrenergic receptor subtype was observed to associate with eIF-2B, a guanine nucleotide exchange protein that functions in regulation of translation. A polymorphic variant of the alpha2B subtype, which lacks 3 glutamic acids from a glutamic acid repeat element, was identified to have decreased G protein-coupled receptor kinase-mediated phosphorylation and desensitization; this polymorphic form is also associated with reduced basal metabolic rate in obese subjects and may therefore contribute to the pathogenesis of obesity. Alpha 2B adrenergic receptor gene contains no introns in either its coding or untranslated sequences.
Cellular localizationCell Membrane
- ADRA2B antibody
- ADRA2RL1 antibody
- ADRARL1 antibody
All lanes : Anti-ADRA2B antibody [EPR9623] (ab151727) at 1/10000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : MCF7 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 50 kDa
Flow cytometric analysis of 293T cells labeling ADRA2B with ab151727 at 1/10 dilution (red) compared to a nonspecific control antibody (green).
This product has been referenced in:
- Maltsev AV et al. a2-Adrenoceptor signaling in cardiomyocytes of spontaneously hypertensive rats starts to impair already at early age. Biochem Biophys Res Commun 512:908-913 (2019). Read more (PubMed: 30929926) »
- Tyurin-Kuzmin PA et al. Flow cytometry analysis of adrenoceptors expression in human adipose-derived mesenchymal stem/stromal cells. Sci Data 5:180196 (2018). Read more (PubMed: 30277480) »