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Signal Transduction Signaling Pathway G Protein Signaling GPCR
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RecombinantRabMAb

Recombinant Anti-ADRA2B antibody [EPR9623] (ab151727)

  • Datasheet
Submit a review Submit a question References (8)

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Western blot - Anti-ADRA2B antibody [EPR9623] (ab151727)
  • Flow Cytometry - Anti-ADRA2B antibody [EPR9623] (ab151727)
  • Anti-ADRA2B antibody [EPR9623] (ab151727)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9623] to ADRA2B
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-ADRA2B antibody [EPR9623]
    See all ADRA2B primary antibodies
  • Description

    Rabbit monoclonal [EPR9623] to ADRA2B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ADRA2B aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P18089
    (Peptide available as ab177733)

  • Positive control

    • Human fetal brain, MCF7, HepG2 and 293T cell lysates; 293T cells.
  • General notes

     

     

     This product was previously labelled as alpha 2b Adrenergic Receptor

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR9623
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Signal Transduction
    • Growth Factors/Hormones
    • Adrenalin

Associated products

  • Alternative Versions

    • Anti-ADRA2B antibody [EPR9623] - BSA and Azide free (ab249044)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

Our Abpromise guarantee covers the use of ab151727 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 50 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Relevance

      Alpha-2-adrenergic receptors are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. Alpha 2B adrenergic receptor subtype was observed to associate with eIF-2B, a guanine nucleotide exchange protein that functions in regulation of translation. A polymorphic variant of the alpha2B subtype, which lacks 3 glutamic acids from a glutamic acid repeat element, was identified to have decreased G protein-coupled receptor kinase-mediated phosphorylation and desensitization; this polymorphic form is also associated with reduced basal metabolic rate in obese subjects and may therefore contribute to the pathogenesis of obesity. Alpha 2B adrenergic receptor gene contains no introns in either its coding or untranslated sequences.
    • Cellular localization

      Cell Membrane
    • Database links

      • Entrez Gene: 151 Human
      • Entrez Gene: 11552 Mouse
      • Entrez Gene: 24174 Rat
      • Omim: 104260 Human
      • SwissProt: P18089 Human
      • SwissProt: P30545 Mouse
      • SwissProt: P19328 Rat
      • Unigene: 247686 Human
      • Unigene: 347390 Mouse
      • Unigene: 10296 Rat
      see all
    • Alternative names

      • ADRA2B antibody
      • ADRA2RL1 antibody
      • ADRARL1 antibody
      • Alpha 2 adrenergic receptor subtype C2 antibody
      • Alpha 2B adrenoceptor antibody
      • Alpha 2B adrenoreceptor antibody
      • alpha 2BAR antibody
      • ALPHA2BAR antibody
      see all

    Images

    • Western blot - Anti-ADRA2B antibody [EPR9623] (ab151727)
      Western blot - Anti-ADRA2B antibody [EPR9623] (ab151727)
      All lanes : Anti-ADRA2B antibody [EPR9623] (ab151727) at 1/10000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 50 kDa

    • Flow Cytometry - Anti-ADRA2B antibody [EPR9623] (ab151727)
      Flow Cytometry - Anti-ADRA2B antibody [EPR9623] (ab151727)

      Flow cytometric analysis of 293T cells labeling ADRA2B with ab151727 at 1/10 dilution (red) compared to a nonspecific control antibody (green).

    • Anti-ADRA2B antibody [EPR9623] (ab151727)
      Anti-ADRA2B antibody [EPR9623] (ab151727)

    Protocols

    • Western blot protocols
    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (8)

    Publishing research using ab151727? Please let us know so that we can cite the reference in this datasheet.

    ab151727 has been referenced in 8 publications.

    • Maltsev AV  et al. a2-Adrenoceptor signaling in cardiomyocytes of spontaneously hypertensive rats starts to impair already at early age. Biochem Biophys Res Commun 512:908-913 (2019). PubMed: 30929926
    • Tyurin-Kuzmin PA  et al. Flow cytometry analysis of adrenoceptors expression in human adipose-derived mesenchymal stem/stromal cells. Sci Data 5:180196 (2018). PubMed: 30277480
    • Wilhelmson AS  et al. Testosterone is an endogenous regulator of BAFF and splenic B cell number. Nat Commun 9:2067 (2018). PubMed: 29802242
    • Tyurin-Kuzmin PA  et al. Noradrenaline Sensitivity Is Severely Impaired in Immortalized Adipose-Derived Mesenchymal Stem Cell Line. Int J Mol Sci 19:N/A (2018). PubMed: 30469522
    • Weng X  et al. Reduced mitochondrial response sensitivity is involved in the anti-apoptotic effect of dexmedetomidine pretreatment in cardiomyocytes. Int J Mol Med 41:2328-2338 (2018). PubMed: 29328437
    • Tyurin-Kuzmin PA  et al. Activation of ß-adrenergic receptors is required for elevated a1A-adrenoreceptors expression and signaling in mesenchymal stromal cells. Sci Rep 6:32835 (2016). Flow Cyt . PubMed: 27596381
    • Xiao L  et al. Renal Denervation Prevents Immune Cell Activation and Renal Inflammation in Angiotensin II-Induced Hypertension. Circ Res 117:547-57 (2015). PubMed: 26156232
    • Maltsev AV  et al. Alpha-2 adrenoceptors and imidazoline receptors in cardiomyocytes mediate counterbalancing effect of agmatine on NO synthesis and intracellular calcium handling. J Mol Cell Cardiol 68:66-74 (2014). PubMed: 24412533

    Customer reviews and Q&As

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