Product nameAnti-Adracalin antibody
See all Adracalin primary antibodies
DescriptionRabbit polyclonal to Adracalin
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Recombinant fragment, corresponding to a region within internal sequence amino acids 172-448 of Human Adracalin (UniProt: Q9NRG9).
- 293T and A431 whole cell lysates; HeLa cells; Human breast carcinoma tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1% BSA, 20% Glycerol, 78% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab152122 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 60 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
RelevanceAdracalin (AAAS) is expressed in both neuroendocrine and cerebral structures and may function in the normal development of the peripheral and central nervous system. It localizes to nuclear pore complexes (NPCs), large multiprotein assemblies that are the sole sites of nucleocytoplasmic transport. Defects in AAAS are the cause of achalasia-addisonianism-alacrima syndrome (AAA syndrome); also known as triple-A syndrome or Allgrove syndrome.
Cellular localizationnuclear pore
- AAA antibody
- AAAS antibody
- AAASb antibody
All lanes : Anti-Adracalin antibody (ab152122) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 60 kDa
7.5% SDS PAGE
Immunofluorescent analysis of methanol-fixed HeLa cells, labeling Adracalin with ab152122 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue, labeling Adracalin with ab152122 at 1/500 dilution.
ab152122 has not yet been referenced specifically in any publications.