Key features and details
- Rabbit polyclonal to Adracalin - C-terminal
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Adracalin antibody - C-terminal
See all Adracalin primary antibodies
DescriptionRabbit polyclonal to Adracalin - C-terminal
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla
- HeLa, 293T, Jurkat, TCMK1 and NIH 3T3 whole cell lysates.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab192911 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 60 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
RelevanceAdracalin (AAAS) is expressed in both neuroendocrine and cerebral structures and may function in the normal development of the peripheral and central nervous system. It localizes to nuclear pore complexes (NPCs), large multiprotein assemblies that are the sole sites of nucleocytoplasmic transport. Defects in AAAS are the cause of achalasia-addisonianism-alacrima syndrome (AAA syndrome); also known as triple-A syndrome or Allgrove syndrome.
Cellular localizationnuclear pore
- AAA antibody
- AAAS antibody
- AAASb antibody
All lanes : Anti-Adracalin antibody - C-terminal (ab192911) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK1 whole cell lysate
Lane 5 : NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 60 kDa
Exposure time: 30 seconds
Lysates prepared using NETN lysis buffer.
Detection of Adracalin by Western Blot of Immunprecipitate.
Lane 1: ab192911 at 1 µg/ml labeling Adracalin in 293T whole cell lysate immunoprecipitated using ab192911 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded).
Lane 2: Control IgG.
Detection: Chemiluminescence with exposure time of 30 seconds.
ab192911 has not yet been referenced specifically in any publications.