Recombinant Anti-ADX antibody [EPR4630] (ab109312)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4630] to ADX
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-ADX antibody [EPR4630]
See all ADX primary antibodies -
Description
Rabbit monoclonal [EPR4630] to ADX -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- Human placenta, Human thyroid and A549 lysates; A549 cells
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4630 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109312 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 14 kDa (predicted molecular weight: 19 kDa).
|
|
ICC/IF |
1/50 - 1/100.
|
Notes |
---|
WB
1/1000 - 1/10000. Detects a band of approximately 14 kDa (predicted molecular weight: 19 kDa). |
ICC/IF
1/50 - 1/100. |
Target
-
Function
Participates in the synthesis of thyroid hormones. Transfers electrons from adrenodoxin reductase to the cholesterol side chain cleavage cytochrome P450. -
Sequence similarities
Belongs to the adrenodoxin/putidaredoxin family.
Contains 1 2Fe-2S ferredoxin-type domain. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
-
Database links
- Entrez Gene: 2230 Human
- Entrez Gene: 14148 Mouse
- Entrez Gene: 29189 Rat
- Omim: 103260 Human
- SwissProt: P10109 Human
- SwissProt: P46656 Mouse
- SwissProt: P24483 Rat
- Unigene: 744 Human
see all -
Alternative names
- Adrenal ferredoxin antibody
- Adrenodoxin antibody
- Adrenodoxin, mitochondrial antibody
see all
Images
-
All lanes : Anti-ADX antibody [EPR4630] (ab109312) at 1/1000 dilution
Lane 1 : Human placenta lysate
Lane 2 : Human thyroid lysate
Lane 3 : A549 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 19 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted? -
Immunofluorescent analysis of ADX in A549 cells using ab109312 at 1/50 dilution.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab109312 has not yet been referenced specifically in any publications.