Overview

  • Product name
    Anti-Adrenomedullin / ADM antibody [HTA171/E8]
    See all Adrenomedullin / ADM primary antibodies
  • Description
    Mouse monoclonal [HTA171/E8] to Adrenomedullin / ADM
  • Host species
    Mouse
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Adrenomedullin/ ADM.

  • General notes

    Previously labelled as Adrenomedullin. 

Properties

Applications

Our Abpromise guarantee covers the use of ab18092 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.

ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50. PubMed: 18445660
IHC-Fr Use at an assay dependent concentration.

Target

  • Function
    AM and PAMP are potent hypotensive and vasodilatator agents. Numerous actions have been reported most related to the physiologic control of fluid and electrolyte homeostasis. In the kidney, am is diuretic and natriuretic, and both am and pamp inhibit aldosterone secretion by direct adrenal actions. In pituitary gland, both peptides at physiologically relevant doses inhibit basal ACTH secretion. Both peptides appear to act in brain and pituitary gland to facilitate the loss of plasma volume, actions which complement their hypotensive effects in blood vessels.
  • Tissue specificity
    Highest levels found in pheochromocytoma and adrenal medulla. Also found in lung, ventricle and kidney tissues.
  • Sequence similarities
    Belongs to the adrenomedullin family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adm antibody
    • ADM precursor antibody
    • ADML_HUMAN antibody
    • Adrenomedullin antibody
    • AM antibody
    • PAMP antibody
    • Preproadrenomedullin antibody
    • Proadrenomedullin N-20 terminal peptide antibody
    • Proadrenomedullin N20 terminal peptide antibody
    • ProAM N terminal 20 peptide antibody
    • ProAM N-terminal 20 peptide antibody
    • ProAM N20 antibody
    • ProAM-N20 antibody
    • ProAMN20 antibody
    see all

Images

  • Overlay histogram showing PC12 cells stained with ab18092 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18092, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgM (mu chain) (ab97007) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References

This product has been referenced in:
  • Kraus DM  et al. Cigarette Smoke-Induced Placental Adrenomedullin Expression and Trophoblast Cell Invasion. Reprod Sci N/A:N/A (2013). Read more (PubMed: 23653390) »
  • Petalidis LP  et al. Improved grading and survival prediction of human astrocytic brain tumors by artificial neural network analysis of gene expression microarray data. Mol Cancer Ther : (2008). IHC-P ; Human . Read more (PubMed: 18445660) »
See all 3 Publications for this product

Customer reviews and Q&As

Question

I have recentny purchasesd this antibody but I am difficulty detecting any protein in my samples by a western. I have also purchased chemically synthesised adrenomedullin from Bachem and am unable to detect a band for this with a western either. Here is my protocol. The samples were boiled for 5 minutes before loading onto a SDS-18% polyacrylamide gel. The gel was overlaid with water saturated butanol and after the gel had polymerised a 5% stacking gel was added to the top. 5?l of Rainbow markers (Amersham) were used in every gel. The voltage was then applied at 100V and left to run for 2hr or until the dye front had reached the bottom of the gel. Proteins were transferred by semi-dry Western blotting onto Hybond-ECL membranes (Amersham). The transfer was undertaken for 30 min at 10 Volts. The membrane was blocked with 5% w/v dried milk in TBS Tween 20 (0.1%) for 1 hour. The blot was incubated with the antibody at a concentrations of 1:1000 up to 1:250 diluted in 5% w/v dried milk in TBS Tween overnight at 4?C. The nitrocellulose was then washed three times with TBS Tween (0.1% v/v) . The secondary antibody, goat anti mouse (Dako) was then added at a concentration of 1:2,000 diluted in 5% dried milk in TBS Tween 20 and incubated for 45 min at RT. Chemiluminescent detection was carried out using ECL reagent (Amersham) Are they any changes you can recommend to my protocol? I have tried both tris-glycine and tris-tricine gels and it didn't seem to make any difference. Otherwise would I be able to get my money back? Thanks for your help

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Answer

Thank you for contacting us for technical support with ab18092. This antibody has not been tested in western blotting, only in immunohistochemistry, and your protocol looks very good so this suggest to me that the antibody may only recognise the native form of the protein in IHC. I would recommend looking at trying non denaturing conditions if you are not using those already (I was unable to work out if you used denatured conditions or not from your protocol). As this antibody has not been tested in western blotting we do not offer a guarantee that it will work in this application, but I hope the above suggestion will help you. Please do not hesitate to contact me again if I can be of further assistance,

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