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Rabbit polyclonal to ADRM1
Predicted to work with:
Chimpanzee, Rhesus monkey, Orangutan
Synthetic peptide, corresponding to a region within amino acids 357-407 of Human ADRM1 (NP_008933.2).
HeLa and 293T whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate, pH 7-8
Concentration information loading...
Immunogen affinity purified
ab91567 was affinity purified using an epitope specific to ADRM1 immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 5-15 µg/mg of lysate.
1/2000 - 1/10000. Predicted molecular weight: 42 kDa.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Functions as a proteasomal ubiquitin receptor. Recruits the deubiquitinating enzyme UCHL5 at the 26S proteasome and promotes its activity.
Belongs to the ADRM1 family.
Contains 1 PH domain.
The PH domain mediates interactions with PSMD1 and ubiquitin. Preferential binding to the proximal subunit of K48-linked diubiquitin allows UCHL5 access to the distal subunit.
Information by UniProt
110 kDa cell membrane glycoprotein antibody
adhesion regulating molecule 1 antibody
Adhesion-regulating molecule 1 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRM1 antibody (ab91567)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling ADRM1 with ab91567 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - Anti-ADRM1 antibody (ab91567)
All lanes : Anti-ADRM1 antibody (ab91567) at 0.1 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Developed using the ECL technique. Predicted band size: 42 kDa Exposure time: 3 minutes
Immunoprecipitation - Anti-ADRM1 antibody (ab91567)
Detection of ADRM1 by Western Blot of Immunprecipitate.
ab91567, at 1 µg/ml, staining ADRM1 in HeLa whole cell lysate immunoprecipitated using ab91567 at 10 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
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