Overview

  • Product name
    Anti-ADRM1 antibody [EPR11449(B)]
    See all ADRM1 primary antibodies
  • Description
    Rabbit monoclonal [EPR11449(B)] to ADRM1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human ADRM1 (UniProt Q16186).

  • Positive control
    • K562, HeLa and Raji cell lysates; Permeabilized K562 cells; Immunoprecipitation pellet from Raji cell lysate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab157185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 42 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Functions as a proteasomal ubiquitin receptor. Recruits the deubiquitinating enzyme UCHL5 at the 26S proteasome and promotes its activity.
  • Sequence similarities
    Belongs to the ADRM1 family.
    Contains 1 PH domain.
  • Domain
    The PH domain mediates interactions with PSMD1 and ubiquitin. Preferential binding to the proximal subunit of K48-linked diubiquitin allows UCHL5 access to the distal subunit.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 110 kDa cell membrane glycoprotein antibody
    • adhesion regulating molecule 1 antibody
    • Adhesion-regulating molecule 1 antibody
    • ADRM 1 antibody
    • Adrm1 antibody
    • ADRM1_HUMAN antibody
    • ARM 1 antibody
    • ARM-1 antibody
    • ARM1 antibody
    • Gp110 antibody
    • hRpn13 antibody
    • M(r) 110,000 surface antigen antibody
    • Proteasomal ubiquitin receptor ADRM1 antibody
    • proteasome regulatory particle non ATPase 13 antibody
    • Proteasome regulatory particle non-ATPase 13 antibody
    • Regulatory particle non ATPase 13 antibody
    • Rpn13 antibody
    • Rpn13 homolog antibody
    see all

Images

  • All lanes : Anti-ADRM1 antibody [EPR11449(B)] (ab157185) at 1/10000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Raji cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 42 kDa

  • Flow cytometric analysis of permeabilized K562 cells labeling ADRM1 with ab157185 at 1/100 dilution (red), compared to a rabbit IgG negative control (green).
  • Anti-ADRM1 antibody [EPR11449(B)] (ab157185) at 1/10000 dilution + immunoprecipitation pellet from Raji cell lysate

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 42 kDa

References

This product has been referenced in:
  • Lu X  et al. Structure of the Rpn13-Rpn2 complex provides insights for Rpn13 and Uch37 as anticancer targets. Nat Commun 8:15540 (2017). Read more (PubMed: 28598414) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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