Overview

  • Product name
    Anti-ADRM1 antibody [EPR11450(B)]
    See all ADRM1 primary antibodies
  • Description
    Rabbit monoclonal [EPR11450(B)] to ADRM1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to a region within Human ADRM1 (UniProt ID: Q16186).

  • Positive control
    • Raji, HeLa, Raw264.7 and NIH 3T3 cell lysates; Human testis tissue, Human thyroid gland carcinoma tissue; HeLa cells.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR11450(B)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab157218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Functions as a proteasomal ubiquitin receptor. Recruits the deubiquitinating enzyme UCHL5 at the 26S proteasome and promotes its activity.
    • Sequence similarities
      Belongs to the ADRM1 family.
      Contains 1 PH domain.
    • Domain
      The PH domain mediates interactions with PSMD1 and ubiquitin. Preferential binding to the proximal subunit of K48-linked diubiquitin allows UCHL5 access to the distal subunit.
    • Cellular localization
      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • 110 kDa cell membrane glycoprotein antibody
      • adhesion regulating molecule 1 antibody
      • Adhesion-regulating molecule 1 antibody
      • ADRM 1 antibody
      • Adrm1 antibody
      • ADRM1_HUMAN antibody
      • ARM 1 antibody
      • ARM-1 antibody
      • ARM1 antibody
      • Gp110 antibody
      • hRpn13 antibody
      • M(r) 110,000 surface antigen antibody
      • Proteasomal ubiquitin receptor ADRM1 antibody
      • proteasome regulatory particle non ATPase 13 antibody
      • Proteasome regulatory particle non-ATPase 13 antibody
      • Regulatory particle non ATPase 13 antibody
      • Rpn13 antibody
      • Rpn13 homolog antibody
      see all

    Images

    • All lanes : Anti-ADRM1 antibody [EPR11450(B)] (ab157218) at 1/1000 dilution

      Lane 1 : Raji cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Raw264.7 cell lysate
      Lane 4 : NIH 3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 42 kDa

    • Immunohistochemical analysis of paraffin embedded Human testis tissue labeling ADRM1 with ab157218 at 1/50.
    • Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue labeling ADRM1 with ab157218 at 1/50.
    • Flow cytometric analysis of permeabilized Hela cells using ab157218 at 1/10 (red) or a rabbit IgG negative (green).

    References

    This product has been referenced in:
    • Huang ZN  et al. Adhesion Regulating Molecule 1 Mediates HAP40 Overexpression-Induced Mitochondrial Defects. Int J Biol Sci 13:1420-1437 (2017). Read more (PubMed: 29209146) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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