Anti-ADRM1/ARM-1 antibody (ab236774)
Key features and details
- Rabbit polyclonal to ADRM1/ARM-1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-ADRM1/ARM-1 antibody
See all ADRM1/ARM-1 primary antibodies -
Description
Rabbit polyclonal to ADRM1/ARM-1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow -
Immunogen
Recombinant full length protein corresponding to Human ADRM1/ARM-1 aa 1 to the C-terminus.
Database link: Q16186 -
Positive control
- WB: Mouse stomach tissue lysate. IHC-P: Human testis tissue. ICC/IF: MCF7 cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
-
Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236774 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/50 - 1/200.
|
|
IHC-P |
1/20 - 1/200.
|
|
WB |
1/1000 - 1/5000. Predicted molecular weight: 42 kDa.
|
Notes |
---|
ICC/IF
1/50 - 1/200. |
IHC-P
1/20 - 1/200. |
WB
1/1000 - 1/5000. Predicted molecular weight: 42 kDa. |
Target
-
Function
Functions as a proteasomal ubiquitin receptor. Recruits the deubiquitinating enzyme UCHL5 at the 26S proteasome and promotes its activity. -
Sequence similarities
Belongs to the ADRM1 family.
Contains 1 PH domain. -
Domain
The PH domain mediates interactions with PSMD1 and ubiquitin. Preferential binding to the proximal subunit of K48-linked diubiquitin allows UCHL5 access to the distal subunit. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 11047 Human
- Entrez Gene: 56436 Mouse
- Entrez Gene: 65138 Rat
- Omim: 610650 Human
- SwissProt: A1L5A6 Cow
- SwissProt: Q16186 Human
- SwissProt: Q9JKV1 Mouse
- SwissProt: Q9JMB5 Rat
see all -
Alternative names
- 110 kDa cell membrane glycoprotein antibody
- adhesion regulating molecule 1 antibody
- Adhesion-regulating molecule 1 antibody
see all
Images
-
Anti-ADRM1/ARM-1 antibody (ab236774) at 1/1000 dilution + Mouse stomach tissue lysate
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 42 kDa -
Paraffin-embedded human testis tissue stained for ADRM1/ARM-1 with ab236774 at 1/100 dilution in immunohistochemical analysis.
-
MCF7 (Human breast adenocarcinoma cell line) cells stained for ADRM1/ARM-1 (Green) using ab236774 at 1/100 dilution in ICC/IF. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab236774 has not yet been referenced specifically in any publications.