Product nameAnti-ADRM1/ARM-1 antibody
See all ADRM1/ARM-1 primary antibodies
DescriptionRabbit polyclonal to ADRM1/ARM-1
Tested applicationsSuitable for: IP, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Chimpanzee, Rhesus monkey, Orangutan
Synthetic peptide corresponding to Human ADRM1/ARM-1 aa 357-407.
Database link: NP_008933.2
- HeLa and 293T whole cell lysates.
This product was previously labelled as ADRM1
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab91567 was affinity purified using an epitope specific to ADRM1/ARM-1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab91567 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 5-15 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 42 kDa.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionFunctions as a proteasomal ubiquitin receptor. Recruits the deubiquitinating enzyme UCHL5 at the 26S proteasome and promotes its activity.
Sequence similaritiesBelongs to the ADRM1 family.
Contains 1 PH domain.
DomainThe PH domain mediates interactions with PSMD1 and ubiquitin. Preferential binding to the proximal subunit of K48-linked diubiquitin allows UCHL5 access to the distal subunit.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- 110 kDa cell membrane glycoprotein antibody
- adhesion regulating molecule 1 antibody
- Adhesion-regulating molecule 1 antibody
All lanes : Anti-ADRM1/ARM-1 antibody (ab91567) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 42 kDa
Exposure time: 3 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling ADRM1/ARM-1 with ab91567 at 1/1000 (1µg/ml). Detection: DAB.
Detection of ADRM1/ARM-1 by Western Blot of Immunprecipitate.
ab91567, at 1 µg/ml, staining ADRM1/ARM-1 in HeLa whole cell lysate immunoprecipitated using ab91567 at 10 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
This product has been referenced in:
- Huang Y & Ratovitski EA Phosphorylated TP63 induces transcription of RPN13, leading to NOS2 protein degradation. J Biol Chem 285:41422-31 (2010). WB ; Human . Read more (PubMed: 20959455) »
- Huang Y & Ratovitski EA Phospho-?Np63a/Rpn13-dependent regulation of LKB1 degradation modulates autophagy in cancer cells. Aging (Albany NY) 2:959-68 (2010). Read more (PubMed: 21191146) »