Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)

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Western blot - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)
  • Flow Cytometry - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)
  • Western blot - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)
  • Western blot - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)
  • Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11449(B)] to ADRM1/ARM-1
  • Suitable for: WB, IHC-P, IP, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-ADRM1/ARM-1 antibody [EPR11449(B)]
    See all ADRM1/ARM-1 primary antibodies

  • Description

    Rabbit monoclonal [EPR11449(B)] to ADRM1/ARM-1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IP, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide corresponding to Human ADRM1/ARM-1 aa 100-200.

  • Positive control

    • WB: HEK293T, K562, HeLa and Raji cell lysates; Immunoprecipitation pellet from Raji cell lysate. Flow Cyt: Permeabilized K562 cells. IHC-P: Human hepatocellular carcinoma

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     This product was previously labelled as ADRM1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR11449(B)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab157185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 42 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Functions as a proteasomal ubiquitin receptor. Recruits the deubiquitinating enzyme UCHL5 at the 26S proteasome and promotes its activity.

  • Sequence similarities

    Belongs to the ADRM1 family.
    Contains 1 PH domain.

  • Domain

    The PH domain mediates interactions with PSMD1 and ubiquitin. Preferential binding to the proximal subunit of K48-linked diubiquitin allows UCHL5 access to the distal subunit.

  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • 110 kDa cell membrane glycoprotein antibody
      • adhesion regulating molecule 1 antibody
      • Adhesion-regulating molecule 1 antibody
      • ADRM 1 antibody
      • Adrm1 antibody
      • ADRM1_HUMAN antibody
      • ARM 1 antibody
      • ARM-1 antibody
      • ARM1 antibody
      • Gp110 antibody
      • hRpn13 antibody
      • M(r) 110,000 surface antigen antibody
      • Proteasomal ubiquitin receptor ADRM1 antibody
      • proteasome regulatory particle non ATPase 13 antibody
      • Proteasome regulatory particle non-ATPase 13 antibody
      • Regulatory particle non ATPase 13 antibody
      • Rpn13 antibody
      • Rpn13 homolog antibody
      see all

    Images

    • Western blot - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)
      Western blot - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)
      All lanes : Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185) at 1/1000 dilution

      Lane 1 : Wild-type HEK293T cell lysate
      Lane 2 : ADRM1 knockout HEK293T cell lysate
      Lane 3 : K-562 cell lysate
      Lane 4 : Raji cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

      Predicted band size: 42 kDa
      Observed band size: 42 kDa



      Lanes 1-4: Merged signal (red and green). Green - ab157185 observed at 42 kDa. Red - loading control ab8245 observed at 36 kDa.

       ab157185 Anti-ADRM1/ARM-1 antibody [EPR11449(B)] was shown to specifically react with ADRM1/ARM-1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266765 (knockout cell lysate ab257816) was used. Wild-type and ADRM1/ARM-1 knockout samples were subjected to SDS-PAGE. ab157185 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)
      Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue sections labeling ADRM1/ARM-1 with purified ab157185 at 1/50 dilution. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Sections were counterstained with Hematoxylin.
      Antigen retrieval was heat mediated antigen retrieval using citrate buffer, pH 6.0).
    • Flow Cytometry - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)
      Flow Cytometry - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)

      Flow cytometric analysis of permeabilized K562 cells labeling ADRM1/ARM-1 with ab157185 at 1/100 dilution (red), compared to a rabbit IgG negative control (green).

    • Western blot - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)
      Western blot - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)
      All lanes : Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185) at 1/10000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 42 kDa

    • Western blot - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)
      Western blot - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)
      Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185) at 1/10000 dilution + immunoprecipitation pellet from Raji cell lysate

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 42 kDa

    • Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)
      Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)

    References (1)

    Publishing research using ab157185? Please let us know so that we can cite the reference in this datasheet.

    ab157185 has been referenced in 1 publication.

    • Lu X  et al. Structure of the Rpn13-Rpn2 complex provides insights for Rpn13 and Uch37 as anticancer targets. Nat Commun 8:15540 (2017). PubMed: 28598414

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