Recombinant
RabMAb

Recombinant Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)

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Overview

  • Product name

    Anti-ADRM1/ARM-1 antibody [EPR11449(B)]
    See all ADRM1/ARM-1 primary antibodies

  • Description

    Rabbit monoclonal [EPR11449(B)] to ADRM1/ARM-1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide corresponding to Human ADRM1/ARM-1 aa 100-200.

  • Positive control

    • K562, HeLa and Raji cell lysates; Permeabilized K562 cells; Immunoprecipitation pellet from Raji cell lysate.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     This product was previously labelled as ADRM1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab157185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 42 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Functions as a proteasomal ubiquitin receptor. Recruits the deubiquitinating enzyme UCHL5 at the 26S proteasome and promotes its activity.

  • Sequence similarities

    Belongs to the ADRM1 family.
    Contains 1 PH domain.

  • Domain

    The PH domain mediates interactions with PSMD1 and ubiquitin. Preferential binding to the proximal subunit of K48-linked diubiquitin allows UCHL5 access to the distal subunit.

  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • 110 kDa cell membrane glycoprotein antibody
      • adhesion regulating molecule 1 antibody
      • Adhesion-regulating molecule 1 antibody
      • ADRM 1 antibody
      • Adrm1 antibody
      • ADRM1_HUMAN antibody
      • ARM 1 antibody
      • ARM-1 antibody
      • ARM1 antibody
      • Gp110 antibody
      • hRpn13 antibody
      • M(r) 110,000 surface antigen antibody
      • Proteasomal ubiquitin receptor ADRM1 antibody
      • proteasome regulatory particle non ATPase 13 antibody
      • Proteasome regulatory particle non-ATPase 13 antibody
      • Regulatory particle non ATPase 13 antibody
      • Rpn13 antibody
      • Rpn13 homolog antibody
      see all

    Images

    • Western blot - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)
      Western blot - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)
      All lanes : Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185) at 1/10000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 42 kDa

    • Flow Cytometry - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)
      Flow Cytometry - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)

      Flow cytometric analysis of permeabilized K562 cells labeling ADRM1/ARM-1 with ab157185 at 1/100 dilution (red), compared to a rabbit IgG negative control (green).

    • Western blot - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)
      Western blot - Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)
      Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185) at 1/10000 dilution + immunoprecipitation pellet from Raji cell lysate

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 42 kDa

    References

    This product has been referenced in:

    • Lu X  et al. Structure of the Rpn13-Rpn2 complex provides insights for Rpn13 and Uch37 as anticancer targets. Nat Commun 8:15540 (2017). Read more (PubMed: 28598414) »
    See 1 Publication for this product

    Publishing research using ab157185? Please let us know so that we can cite the reference in this datasheet.

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