Product nameAnti-ADRM1/ARM-1 antibody [EPR11450(B)]
See all ADRM1/ARM-1 primary antibodies
DescriptionRabbit monoclonal [EPR11450(B)] to ADRM1/ARM-1
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human ADRM1/ARM-1 aa 350-450.
- Raji, HeLa, Raw264.7 and NIH 3T3 cell lysates; Human testis tissue, Human thyroid gland carcinoma tissue; HeLa cells.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product was previously labelled as ADRM1
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab157218 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 42 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/250.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionFunctions as a proteasomal ubiquitin receptor. Recruits the deubiquitinating enzyme UCHL5 at the 26S proteasome and promotes its activity.
Sequence similaritiesBelongs to the ADRM1 family.
Contains 1 PH domain.
DomainThe PH domain mediates interactions with PSMD1 and ubiquitin. Preferential binding to the proximal subunit of K48-linked diubiquitin allows UCHL5 access to the distal subunit.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- 110 kDa cell membrane glycoprotein antibody
- adhesion regulating molecule 1 antibody
- Adhesion-regulating molecule 1 antibody
All lanes : Anti-ADRM1/ARM-1 antibody [EPR11450(B)] (ab157218) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Raw264.7 cell lysate
Lane 4 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 42 kDa
Immunohistochemical analysis of paraffin embedded Human testis tissue labeling ADRM1/ARM-1 with ab157218 at 1/50.
Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue labeling ADRM1/ARM-1 with ab157218 at 1/50.
Flow cytometric analysis of permeabilized Hela cells using ab157218 at 1/10 (red) or a rabbit IgG negative (green).
This product has been referenced in:
- Feng Q et al. Quantitative Proteomic Analysis Reveals That Arctigenin Alleviates Concanavalin A-Induced Hepatitis Through Suppressing Immune System and Regulating Autophagy. Front Immunol 9:1881 (2018). Read more (PubMed: 30177931) »
- Huang ZN et al. Adhesion Regulating Molecule 1 Mediates HAP40 Overexpression-Induced Mitochondrial Defects. Int J Biol Sci 13:1420-1437 (2017). Read more (PubMed: 29209146) »