Product nameAnti-AdSS 2 antibody [EPR12331-52]
See all AdSS 2 primary antibodies
DescriptionRabbit monoclonal [EPR12331-52] to AdSS 2
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC or IHC
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Recombinant fragment corresponding to Human AdSS 2.
Database link: P30520
- 293T, HT1376 and HepG2 cell lysates.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab174842 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 50 kDa.|
|IP||1/10 - 1/100.|
FunctionPlays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP.
PathwayPurine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.
Sequence similaritiesBelongs to the adenylosuccinate synthetase family.
Cellular localizationCytoplasm. Partially associated with particulate fractions.
- Information by UniProt
- (Ade(-)H, complement of antibody
- acidic isozyme antibody
- ADEH antibody
All lanes : Anti-AdSS 2 antibody [EPR12331-52] (ab174842) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HT1376 cell lysate
Lane 3 : HepG2 cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 50 kDa
Western blot analysis on immunoprecipitation pellet from 293T cell lysate (lane 1) or 1X PBS (lane 2) (negative control), labeling AdSS 2 immunoprecipitated using ab174842 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
ab174842 has not yet been referenced specifically in any publications.