Product nameAnti-AdSS 2 antibody [EPR12331-73]
See all AdSS 2 primary antibodies
DescriptionRabbit monoclonal [EPR12331-73] to AdSS 2
Tested applicationsSuitable for: ICC/IF, Flow Cyt, WBmore details
Unsuitable for: IHC
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human AdSS 2. The exact sequence is proprietary.
Database link: P30520
- 293T, HT-1376, HepG2 and U87-MG cell lysates; 293 cells; permeabilized 293 cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab174848 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/1000 - 1/5000. Predicted molecular weight: 50 kDa.|
FunctionPlays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP.
PathwayPurine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.
Sequence similaritiesBelongs to the adenylosuccinate synthetase family.
Cellular localizationCytoplasm. Partially associated with particulate fractions.
- Information by UniProt
- (Ade(-)H, complement of antibody
- acidic isozyme antibody
- ADEH antibody
All lanes : Anti-AdSS 2 antibody [EPR12331-73] (ab174848) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HT-1376 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : U87-MG cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 50 kDa
Immunofluorescence analysis of 293 cells labeling AdSS 2 using ab174848 at a 1/50 dilution. DAPI nuclear staining (blue).
Flow cytometric analysis of permeabilized 293 cells labeling AdSS 2 using ab174848 at a 1/10 dilution (red), or a rabbit IgG negative control (green).
ab174848 has not yet been referenced specifically in any publications.