Overview

  • Product name
    Anti-AdSS 2 antibody [EPR12331-73]
    See all AdSS 2 primary antibodies
  • Description
    Rabbit monoclonal [EPR12331-73] to AdSS 2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, WBmore details
    Unsuitable for: IHC
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human AdSS 2. The exact sequence is proprietary.
    Database link: P30520

  • Positive control
    • 293T, HT-1376, HepG2 and U87-MG cell lysates; 293 cells; permeabilized 293 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR12331-73
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB 1/1000 - 1/5000. Predicted molecular weight: 50 kDa.
  • Application notes
    Is unsuitable for IHC.
  • Target

    • Function
      Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP.
    • Pathway
      Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.
    • Sequence similarities
      Belongs to the adenylosuccinate synthetase family.
    • Cellular localization
      Cytoplasm. Partially associated with particulate fractions.
    • Information by UniProt
    • Database links
    • Alternative names
      • (Ade(-)H, complement of antibody
      • acidic isozyme antibody
      • ADEH antibody
      • Adenylosuccinate synthetase (Ade(-)H complementing) antibody
      • Adenylosuccinate synthetase acidic isozyme antibody
      • Adenylosuccinate synthetase antibody
      • Adenylosuccinate synthetase isozyme 2 antibody
      • Adenylosuccinate synthetase liver isozyme antibody
      • AdSS 2 antibody
      • ADSS antibody
      • AMPSase 2 antibody
      • IMP--aspartate ligase 2 antibody
      • L-type adenylosuccinate synthetase antibody
      • liver isozyme antibody
      • PURA2_HUMAN antibody
      see all

    Images

    • All lanes : Anti-AdSS 2 antibody [EPR12331-73] (ab174848) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HT-1376 cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : U87-MG cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 50 kDa

    • Immunofluorescence analysis of 293 cells labeling AdSS 2 using ab174848 at a 1/50 dilution. DAPI nuclear staining (blue).

    • Flow cytometric analysis of permeabilized 293 cells labeling AdSS 2 using ab174848 at a 1/10 dilution (red), or a rabbit IgG negative control (green).

    References

    ab174848 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (C2C12 Cells)
    Gel Running Conditions
    Reduced Denaturing (10% SDS PAGE)
    Loading amount
    62000 cells
    Specification
    C2C12 Cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Dr. Chris Tracy

    Verified customer

    Submitted Sep 03 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up