Recombinant Anti-AdSS 2 antibody [EPR12331-73] (ab174848)
![Western blot - Anti-AdSS 2 antibody [EPR12331-73] (ab174848)](https://www.abcam.com/ps/products/174/ab174848/Images/ab174848-200928-anti-adss-2-antibody-epr12331-73-western-blot.jpg "Western blot - Anti-AdSS 2 antibody [EPR12331-73] (ab174848)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12331-73] to AdSS 2
- Suitable for: ICC/IF, Flow Cyt, WB
- Reacts with: Human
Overview
-
Product name
Anti-AdSS 2 antibody [EPR12331-73]
See all AdSS 2 primary antibodies -
Description
Rabbit monoclonal [EPR12331-73] to AdSS 2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, Flow Cyt, WBmore details
Unsuitable for: IHC -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
-
Immunogen
Recombinant fragment within Human AdSS 2 aa 1-200. The exact sequence is proprietary.
Database link: P30520 -
Positive control
- 293T, HT-1376, HepG2 and U87-MG cell lysates; 293 cells; permeabilized 293 cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12331-73 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab174848 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/50 - 1/100.
|
|
| Flow Cyt |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB | (1) |
1/1000 - 1/5000. Predicted molecular weight: 50 kDa.
|
| Notes |
|---|
|
ICC/IF
1/50 - 1/100. |
|
Flow Cyt
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/5000. Predicted molecular weight: 50 kDa. |
Target
-
Function
Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP. -
Pathway
Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. -
Sequence similarities
Belongs to the adenylosuccinate synthetase family. -
Cellular localization
Cytoplasm. Partially associated with particulate fractions. - Information by UniProt
-
Database links
- Entrez Gene: 159 Human
- Entrez Gene: 11566 Mouse
- Entrez Gene: 289276 Rat
- Omim: 103060 Human
- SwissProt: P30520 Human
- SwissProt: P46664 Mouse
- Unigene: 498313 Human
- Unigene: 338021 Mouse
see all -
Alternative names
- (Ade(-)H, complement of antibody
- acidic isozyme antibody
- ADEH antibody
see all
Images
-
Western blot - Anti-AdSS 2 antibody [EPR12331-73] (ab174848)All lanes : Anti-AdSS 2 antibody [EPR12331-73] (ab174848) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HT-1376 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : U87-MG cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 50 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-AdSS 2 antibody [EPR12331-73] (ab174848)](https://www.abcam.com/ps/products/174/ab174848/Images/ab174848-200927-anti-adss-2-antibody-epr12331-73-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-AdSS 2 antibody [EPR12331-73] (ab174848)Immunofluorescence analysis of 293 cells labeling AdSS 2 using ab174848 at a 1/50 dilution. DAPI nuclear staining (blue).
-
![Flow Cytometry - Anti-AdSS 2 antibody [EPR12331-73] (ab174848)](https://www.abcam.com/ps/products/174/ab174848/Images/ab174848-200926-anti-adss-2-antibody-epr12331-73-flow-cytometry.jpg)
Flow Cytometry - Anti-AdSS 2 antibody [EPR12331-73] (ab174848)Flow cytometric analysis of permeabilized 293 cells labeling AdSS 2 using ab174848 at a 1/10 dilution (red), or a rabbit IgG negative control (green).
-
![Anti-AdSS 2 antibody [EPR12331-73] (ab174848)](https://www.abcam.com/ps/products/174/ab174848/Images/ab174848-4-benefits-of-recombinant-antibodies.png)
Anti-AdSS 2 antibody [EPR12331-73] (ab174848)
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab174848 has not yet been referenced specifically in any publications.