Recombinant
RabMAb

Recombinant Anti-AdSS 2 antibody [EPR12331-73] (ab174848)

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Overview

  • Product name

    Anti-AdSS 2 antibody [EPR12331-73]
    See all AdSS 2 primary antibodies

  • Description

    Rabbit monoclonal [EPR12331-73] to AdSS 2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, WBmore details
    Unsuitable for: IHC

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human AdSS 2 aa 1-200. The exact sequence is proprietary.
    Database link: P30520

  • Positive control

    • 293T, HT-1376, HepG2 and U87-MG cell lysates; 293 cells; permeabilized 293 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR12331-73

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/1000 - 1/5000. Predicted molecular weight: 50 kDa.
  • Application notes
    Is unsuitable for IHC.

    • Target

    • Function

      Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP.

    • Pathway

      Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.

    • Sequence similarities

      Belongs to the adenylosuccinate synthetase family.

    • Cellular localization

      Cytoplasm. Partially associated with particulate fractions.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • (Ade(-)H, complement of antibody
      • acidic isozyme antibody
      • ADEH antibody
      • Adenylosuccinate synthetase (Ade(-)H complementing) antibody
      • Adenylosuccinate synthetase acidic isozyme antibody
      • Adenylosuccinate synthetase antibody
      • Adenylosuccinate synthetase isozyme 2 antibody
      • Adenylosuccinate synthetase liver isozyme antibody
      • AdSS 2 antibody
      • ADSS antibody
      • AMPSase 2 antibody
      • IMP--aspartate ligase 2 antibody
      • L-type adenylosuccinate synthetase antibody
      • liver isozyme antibody
      • PURA2_HUMAN antibody
      see all

    Images

    • Western blot - Anti-AdSS 2 antibody [EPR12331-73] (ab174848)
      Western blot - Anti-AdSS 2 antibody [EPR12331-73] (ab174848)
      All lanes : Anti-AdSS 2 antibody [EPR12331-73] (ab174848) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HT-1376 cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : U87-MG cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 50 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-AdSS 2 antibody [EPR12331-73] (ab174848)
      Immunocytochemistry/ Immunofluorescence - Anti-AdSS 2 antibody [EPR12331-73] (ab174848)

      Immunofluorescence analysis of 293 cells labeling AdSS 2 using ab174848 at a 1/50 dilution. DAPI nuclear staining (blue).

    • Flow Cytometry - Anti-AdSS 2 antibody [EPR12331-73] (ab174848)
      Flow Cytometry - Anti-AdSS 2 antibody [EPR12331-73] (ab174848)

      Flow cytometric analysis of permeabilized 293 cells labeling AdSS 2 using ab174848 at a 1/10 dilution (red), or a rabbit IgG negative control (green).

    References

    ab174848 has not yet been referenced specifically in any publications.

    Publishing research using ab174848? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Western blot abreview for Anti-AdSS 2 antibody [EPR12331-73]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (C2C12 Cells)
    Gel Running Conditions
    Reduced Denaturing (10% SDS PAGE)
    Loading amount
    62000 cells
    Specification
    C2C12 Cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Dr. Chris Tracy

    Verified customer

    Submitted Sep 03 2015

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