Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4629] to ADX
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Product nameAnti-ADX antibody [EPR4629]
See all ADX primary antibodies
DescriptionRabbit monoclonal [EPR4629] to ADX
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human ADX aa 50-150. The exact sequence is proprietary.
- Human placenta, A549, Human thyroid, and JAR lysates; Human adrenal gland tissue.
This product was previously labelled as Adrenodoxin
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab108257 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 14 kDa (predicted molecular weight: 19 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended.
FunctionParticipates in the synthesis of thyroid hormones. Transfers electrons from adrenodoxin reductase to the cholesterol side chain cleavage cytochrome P450.
Sequence similaritiesBelongs to the adrenodoxin/putidaredoxin family.
Contains 1 2Fe-2S ferredoxin-type domain.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- Adrenal ferredoxin antibody
- Adrenodoxin antibody
- Adrenodoxin, mitochondrial antibody
All lanes : Anti-ADX antibody [EPR4629] (ab108257) at 1/1000 dilution
Lane 1 : Human placenta lysate
Lane 2 : A549 lysate
Lane 3 : Human thyroid lysate
Lane 4 : JAR lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 19 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?
ab108257 at 1/50 dilution staining ADX in Human adrenal gland by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab108257 has been referenced in 3 publications.
- Zhang J et al. FDXR regulates TP73 tumor suppressor via IRP2 to modulate aging and tumor suppression. J Pathol N/A:N/A (2020). PubMed: 32304229
- Zhang Y et al. Ferredoxin reductase is critical for p53-dependent tumor suppression via iron regulatory protein 2. Genes Dev N/A:N/A (2017). WB . PubMed: 28747430
- Li J et al. De novo synthesis of steroids and oxysterols in adipocytes. J Biol Chem 289:747-64 (2014). WB . PubMed: 24280213