Product nameAnti-ADX antibody [EPR4629]
See all ADX primary antibodies
DescriptionRabbit monoclonal [EPR4629] to ADX
Tested applicationsSuitable for: WB, IP, IHC-P, ICCmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human ADX aa 50-150. The exact sequence is proprietary.
- Human placenta, A549, Human thyroid, and JAR lysates; Human adrenal gland tissue.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab108257 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 14 kDa (predicted molecular weight: 19 kDa).|
|IP||1/10 - 1/100.|
|IHC-P||1/50 - 1/100. Antigen retrieval is recommended.|
|ICC||1/50 - 1/100.|
FunctionParticipates in the synthesis of thyroid hormones. Transfers electrons from adrenodoxin reductase to the cholesterol side chain cleavage cytochrome P450.
Sequence similaritiesBelongs to the adrenodoxin/putidaredoxin family.
Contains 1 2Fe-2S ferredoxin-type domain.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- Adrenal ferredoxin antibody
- Adrenodoxin antibody
- Adrenodoxin, mitochondrial antibody
All lanes : Anti-ADX antibody [EPR4629] (ab108257) at 1/1000 dilution
Lane 1 : Human placenta lysate
Lane 2 : A549 lysate
Lane 3 : Human thyroid lysate
Lane 4 : JAR lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 19 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?
ab108257 at 1/50 dilution staining ADX in Human adrenal gland by Immunohistochemistry, Paraffin-embedded tissue.
This product has been referenced in:
- Zhang Y et al. Ferredoxin reductase is critical for p53-dependent tumor suppression via iron regulatory protein 2. Genes Dev N/A:N/A (2017). WB . Read more (PubMed: 28747430) »
- Li J et al. De novo synthesis of steroids and oxysterols in adipocytes. J Biol Chem 289:747-64 (2014). WB . Read more (PubMed: 24280213) »