Product nameAnti-ADX antibody [EPR4630]
See all ADX primary antibodies
DescriptionRabbit monoclonal [EPR4630] to ADX
Tested applicationsSuitable for: IP, WB, ICC/IFmore details
Unsuitable for: Flow Cyt or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human ADX aa 100-200 (internal sequence). The exact sequence is proprietary.
- Human placenta, Human thyroid and A549 lysates; A549 cells
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109312 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/10 - 1/100.|
|WB||1/1000 - 1/10000. Detects a band of approximately 14 kDa (predicted molecular weight: 19 kDa).|
|ICC/IF||1/50 - 1/100.|
FunctionParticipates in the synthesis of thyroid hormones. Transfers electrons from adrenodoxin reductase to the cholesterol side chain cleavage cytochrome P450.
Sequence similaritiesBelongs to the adrenodoxin/putidaredoxin family.
Contains 1 2Fe-2S ferredoxin-type domain.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- Adrenal ferredoxin antibody
- Adrenodoxin antibody
- Adrenodoxin, mitochondrial antibody
All lanes : Anti-ADX antibody [EPR4630] (ab109312) at 1/1000 dilution
Lane 1 : Human placenta lysate
Lane 2 : Human thyroid lysate
Lane 3 : A549 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 19 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?
Immunofluorescent analysis of ADX in A549 cells using ab109312 at 1/50 dilution.
ab109312 has not yet been referenced specifically in any publications.