Recombinant Anti-ADX antibody [EPR4630] (ab109312)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-ADX antibody [EPR4630]
See all ADX primary antibodies -
Description
Rabbit monoclonal [EPR4630] to ADX -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, ICC/IFmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human ADX aa 100-200 (internal sequence). The exact sequence is proprietary.
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Positive control
- Human placenta, Human thyroid and A549 lysates; A549 cells
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General notes
This product was previously labelled as Adrenodoxin
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4630 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab109312 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | 1/10 - 1/100. | |
| WB | 1/1000 - 1/10000. Detects a band of approximately 14 kDa (predicted molecular weight: 19 kDa). | |
| ICC/IF | 1/50 - 1/100. |
Target
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Function
Participates in the synthesis of thyroid hormones. Transfers electrons from adrenodoxin reductase to the cholesterol side chain cleavage cytochrome P450. -
Sequence similarities
Belongs to the adrenodoxin/putidaredoxin family.
Contains 1 2Fe-2S ferredoxin-type domain. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 2230 Human
- Entrez Gene: 14148 Mouse
- Entrez Gene: 29189 Rat
- Omim: 103260 Human
- SwissProt: P10109 Human
- SwissProt: P46656 Mouse
- SwissProt: P24483 Rat
- Unigene: 744 Human
see all -
Alternative names
- Adrenal ferredoxin antibody
- Adrenodoxin antibody
- Adrenodoxin, mitochondrial antibody
see all
Images
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![Western blot - Anti-ADX antibody [EPR4630] (ab109312)](https://www.abcam.com/ps/products/109/ab109312/Images/ab109312-122079-anti-adrenodoxin-antibody-epr4630-western-blot.jpg)
Western blot - Anti-ADX antibody [EPR4630] (ab109312)All lanes : Anti-ADX antibody [EPR4630] (ab109312) at 1/1000 dilution
Lane 1 : Human placenta lysate
Lane 2 : Human thyroid lysate
Lane 3 : A549 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 19 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted? -
![Immunocytochemistry/ Immunofluorescence - Anti-ADX antibody [EPR4630] (ab109312)](https://www.abcam.com/ps/products/109/ab109312/Images/ab109312-122078-anti-adrenodoxin-antibody-epr4630-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ADX antibody [EPR4630] (ab109312)Immunofluorescent analysis of ADX in A549 cells using ab109312 at 1/50 dilution.
Protocols
Datasheets and documents
References
ab109312 has not yet been referenced specifically in any publications.
