Recombinant Anti-AE binding protein 1 antibody [EPR7733(2)] (ab168355)
![Western blot - Anti-AE binding protein 1 antibody [EPR7733(2)] (ab168355)](https://www.abcam.com/ps/products/168/ab168355/Images/ab168355-166629-anti-ae-binding-protein-1-antibody-epr77332-western-blot.jpg "Western blot - Anti-AE binding protein 1 antibody [EPR7733(2)] (ab168355)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7733(2)] to AE binding protein 1
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-AE binding protein 1 antibody [EPR7733(2)]
See all AE binding protein 1 primary antibodies -
Description
Rabbit monoclonal [EPR7733(2)] to AE binding protein 1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human AE binding protein 1.
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Positive control
- HepG2 cell lysate.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7733(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab168355 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 131 kDa. |
Target
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Relevance
AEBP1 (adipocyte enhancer binding protein 1) is a transcriptional repressor which binds to the adipocyte enhancer 1 (AE-1) regulatory sequence, located in the proximal promoter region of the adipose P2 (aP2) gene. AEBP1 has carboxypeptidase (CP) activity and the direct binding of DNA to AEBP1 enhances its protease activity. AEBP1 expression is largely restricted to the aorta, particularly to smooth muscle cells. -
Database links
- Entrez Gene: 165 Human
- Omim: 602981 Human
- SwissProt: Q14113 Human
- Unigene: 439463 Human
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Alternative names
- ACLP antibody
- Adipocyte enhancer binding protein 1 antibody
- AEBP 1 antibody
see all
Images
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Western blot - Anti-AE binding protein 1 antibody [EPR7733(2)] (ab168355)Anti-AE binding protein 1 antibody [EPR7733(2)] (ab168355) at 1/1000 dilution + HepG2 cell lysate at 10 µg
Secondary
HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 131 kDa -
![Anti-AE binding protein 1 antibody [EPR7733(2)] (ab168355)](https://www.abcam.com/ps/products/168/ab168355/Images/ab168355-2-benefits-of-recombinant-antibodies.png)
Anti-AE binding protein 1 antibody [EPR7733(2)] (ab168355)
Datasheets and documents
References (1)
ab168355 has been referenced in 1 publication.
- Yorozu A et al. Upregulation of adipocyte enhancer-binding protein 1 in endothelial cells promotes tumor angiogenesis in colorectal cancer. Cancer Sci 111:1631-1644 (2020). PubMed: 32086986
