Key features and details
- Rabbit polyclonal to AE2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-AE2 antibody
See all AE2 primary antibodies
DescriptionRabbit polyclonal to AE2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Sheep, Horse, Cow, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
- HeLa, Jurkat and 293T cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab191189 was affinity purified using an epitope specific to AE2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab191189 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 137 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionPlasma membrane anion exchange protein of wide distribution.
Sequence similaritiesBelongs to the anion exchanger (TC 2.A.31) family.
- Information by UniProt
- AE 2 antibody
- AE2 anion exchanger antibody
- Anion exchange protein 2 antibody
All lanes : Anti-AE2 antibody (ab191189) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 137 kDa
Exposure time: 3 minutes
Detection of AE2 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab191189 at 6 µg/reaction for IP and 1µg/ml for WB detection (Lane 1). Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
ab191189 has not yet been referenced specifically in any publications.