Key features and details
- Rabbit polyclonal to AE2
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-AE2 antibody
See all AE2 primary antibodies
DescriptionRabbit polyclonal to AE2
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Orangutan
Synthetic peptide within Human AE2 aa 1-50. The exact sequence is proprietary. (NP_001186621.1).
Database link: P04920
- WB: HeLa and HEK-293T whole cell lysates. IP: HEK-293T whole cell lysate. IHC-P: Human ovarian carcinoma tissue.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226829 was affinity purified using an epitope specific to AE2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab226829 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 137 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionPlasma membrane anion exchange protein of wide distribution.
Sequence similaritiesBelongs to the anion exchanger (TC 2.A.31) family.
- Information by UniProt
- AE 2 antibody
- AE2 anion exchanger antibody
- Anion exchange protein 2 antibody
Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for AE2 using ab226829 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining.
All lanes : Anti-AE2 antibody (ab226829) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 137 kDa
Exposure time: 30 seconds
Lysates were prepared using RIPA lysis buffer.
AE2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using RIPA lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226829 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226829 at 1 µg/ml.
Lane 1: ab226829 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226829 has not yet been referenced specifically in any publications.