Overview

  • Product name

  • Description

    Rabbit polyclonal to AE2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Orangutan
  • Immunogen

    Synthetic peptide within Human AE2 aa 1-50. The exact sequence is proprietary. (NP_001186621.1).
    Database link: P04920

  • Positive control

    • WB: HeLa and HEK-293T whole cell lysates. IP: HEK-293T whole cell lysate. IHC-P: Human ovarian carcinoma tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab226829 was affinity purified using an epitope specific to AE2 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab226829 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 137 kDa.
IP Use at 2-10 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Plasma membrane anion exchange protein of wide distribution.
  • Sequence similarities

    Belongs to the anion exchanger (TC 2.A.31) family.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AE 2 antibody
    • AE2 anion exchanger antibody
    • Anion exchange protein 2 antibody
    • Anion exchanger 2 antibody
    • B3A2_HUMAN antibody
    • BAND 3-LIKE PROTEIN antibody
    • BND3L antibody
    • EPB3L1 antibody
    • erythrocyte band 3 antibody
    • Erythrocyte membrane protein band 3 like 1 antibody
    • HKB3 antibody
    • MPB3L antibody
    • NBND3 antibody
    • Non-erythroid band 3-like protein antibody
    • Slc4a2 antibody
    • Solute carrier family 4 member 2 antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for AE2 using ab226829 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining. 

  • All lanes : Anti-AE2 antibody (ab226829) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 137 kDa


    Exposure time: 30 seconds


    Lysates were prepared using RIPA lysis buffer.

  • AE2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using RIPA lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226829 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226829 at 1 µg/ml.

    Lane 1: ab226829 IP in HEK-293T whole cell lysate.

    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection: Chemiluminescence with exposure time of 30 seconds.

References

ab226829 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab226829.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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