This fast track antibody is not yet fully characterized. It is subject to these
terms and conditions
- Datasheet
- Specific References (2)
- Protocols
Overview
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Product nameAnti-AE2 antibody
See all AE2 primary antibodies -
DescriptionRabbit polyclonal to AE2
This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »
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Host speciesRabbit
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Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Orangutan
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Immunogen
Synthetic peptide derived from within residues 200 - 300 of Human AE2.
(Peptide available as ab42686.)
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Positive control
- This antibody gave a positive signal when tested on MCF7 cells in IF
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| IHC-FoFr | Use at an assay dependent concentration. PubMed: 21705333 |
Target
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FunctionPlasma membrane anion exchange protein of wide distribution.
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Sequence similaritiesBelongs to the anion exchanger (TC 2.A.31) family.
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Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 404084 Cow
- Entrez Gene: 791243 Horse
- Entrez Gene: 6522 Human
- Entrez Gene: 20535 Mouse
- Entrez Gene: 100172030 Orangutan
- Entrez Gene: 100009159 Rabbit
- Entrez Gene: 24780 Rat
- Omim: 109280 Human
see all -
Alternative names
- AE 2 antibody
- AE2 anion exchanger antibody
- Anion exchange protein 2 antibody
see all
Images
This Fast-Track antibody is not yet fully characterised. These images represent
inconclusive preliminary data.
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Immunocytochemistry/ Immunofluorescence - Anti-AE2 antibody (ab42687)ICC/IF image of ab42687 stained MCF7 cells. The cells were 100% methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab42687, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek293 cells fixed in 100% methanol at 5ug/ml. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
References
This product has been referenced in:
- Yin K et al. SLC26A Gene Family Participate in pH Regulation during Enamel Maturation. PLoS One 10:e0144703 (2015). IHC-P ; Rat . Read more (PubMed: 26671068) »
- Kao L et al. Severe Neurologic Impairment in Mice with Targeted Disruption of the Electrogenic Sodium Bicarbonate Cotransporter NBCe2 (Slc4a5 Gene). J Biol Chem 286:32563-74 (2011). IHC-FoFr ; Mouse . Read more (PubMed: 21705333) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"