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Anti-AE2 antibody (ab42687)

This fast track antibody is not yet fully characterized. It is subject to these terms and conditions.
  • Datasheet
Reviews (2) Submit a question References (5)

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Immunocytochemistry/ Immunofluorescence - Anti-AE2 antibody (ab42687)

    Key features and details

    • Rabbit polyclonal to AE2
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Isotype: IgG

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    Overview

    • Product name

      Anti-AE2 antibody
      See all AE2 primary antibodies
    • Description

      Rabbit polyclonal to AE2

      This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

    • Host species

      Rabbit
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Orangutan
    • Immunogen

      Synthetic peptide corresponding to Human AE2 aa 200-300.
      (Peptide available as ab42686)

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituent: PBS

      Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cardiovascular
      • Blood
      • Other
      • Signal Transduction
      • Metabolism
      • Plasma Membrane
      • Channels
      • Cardiovascular
      • Heart
      • Hypertrophy
      • Other
      • Metabolism
      • Types of disease
      • Cancer

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

    Application Abreviews Notes
    ICC/IF Use at an assay dependent concentration.

    Target

    • Function

      Plasma membrane anion exchange protein of wide distribution.
    • Sequence similarities

      Belongs to the anion exchanger (TC 2.A.31) family.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P04920 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 404084 Cow
      • Entrez Gene: 791243 Horse
      • Entrez Gene: 6522 Human
      • Entrez Gene: 20535 Mouse
      • Entrez Gene: 100172030 Orangutan
      • Entrez Gene: 100009159 Rabbit
      • Entrez Gene: 24780 Rat
      • Omim: 109280 Human
      • SwissProt: Q6SJP2 Horse
      • SwissProt: P04920 Human
      • SwissProt: P13808 Mouse
      • SwissProt: Q5RD44 Orangutan
      • SwissProt: P48746 Rabbit
      • SwissProt: P23347 Rat
      • Unigene: 647069 Human
      • Unigene: 4580 Mouse
      • Unigene: 9860 Rat
      see all
    • Alternative names

      • AE 2 antibody
      • AE2 anion exchanger antibody
      • Anion exchange protein 2 antibody
      • Anion exchanger 2 antibody
      • B3A2_HUMAN antibody
      • BAND 3-LIKE PROTEIN antibody
      • BND3L antibody
      • EPB3L1 antibody
      • erythrocyte band 3 antibody
      • Erythrocyte membrane protein band 3 like 1 antibody
      • HKB3 antibody
      • MPB3L antibody
      • NBND3 antibody
      • Non-erythroid band 3-like protein antibody
      • Slc4a2 antibody
      • Solute carrier family 4 member 2 antibody
      see all

    Images

    This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

    • Immunocytochemistry/ Immunofluorescence - Anti-AE2 antibody (ab42687)
      Immunocytochemistry/ Immunofluorescence - Anti-AE2 antibody (ab42687)
      ICC/IF image of ab42687 stained MCF7 cells. The cells were 100% methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab42687, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek293 cells fixed in 100% methanol at 5ug/ml. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (5)

    Publishing research using ab42687? Please let us know so that we can cite the reference in this datasheet.

    ab42687 has been referenced in 5 publications.

    • Hwang S  et al. Carbonic anhydrase 12 mutation modulates membrane stability and volume regulation of aquaporin 5. J Enzyme Inhib Med Chem 34:179-188 (2019). PubMed: 30451023
    • Hwang S  et al. Drug Repurposing as an Antitumor Agent: Disulfiram-Mediated Carbonic Anhydrase 12 and Anion Exchanger 2 Modulation to Inhibit Cancer Cell Migration. Molecules 24:N/A (2019). PubMed: 31546841
    • Yin K  et al. SLC26A Gene Family Participate in pH Regulation during Enamel Maturation. PLoS One 10:e0144703 (2015). IHC-P ; Rat . PubMed: 26671068
    • Huang J  et al. Basolateral chloride loading by the anion exchanger type 2: role in fluid secretion by the human airway epithelial cell line Calu-3. J Physiol 590:5299-316 (2012). PubMed: 22802585
    • Kao L  et al. Severe Neurologic Impairment in Mice with Targeted Disruption of the Electrogenic Sodium Bicarbonate Cotransporter NBCe2 (Slc4a5 Gene). J Biol Chem 286:32563-74 (2011). IHC-FoFr ; Mouse . PubMed: 21705333

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-AE2 antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Lung, Liver)
    Loading amount
    25 µg
    Specification
    Lung, Liver
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 15 2009

    Western blot abreview for Anti-AE2 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa, U2OS, NIH-3T3, MEF)
    Loading amount
    20 µg
    Specification
    HeLa, U2OS, NIH-3T3, MEF
    Gel Running Conditions
    Reduced Denaturing (7.5% SDS-PAGE)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Mr. Antibody Solutions

    Verified customer

    Submitted Jan 27 2009

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